Journal: Theranostics

Article Title: Specific in vivo detection of V2R-positive metastatic ccRCC using a toxin-based PET radioligand

doi: 10.7150/thno.126311

Figure Lengend Snippet: In vivo visualization of V2R + tumors in mccRCC representative mice models. A: Representative composite images taken at 4 h after the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). Images shown are the result of a fusion between the Computed Tomography (CT) image (grey scale) and the PET image (false colors). B: Summary table of the main PET imaging indicators obtained after 4 hours of the i.v. injection of 20 nmol/kg for the [ 18 F]F-MQ232 in CHO-304 xenografted NMRI-Foxn1 nu/nu mice (6.8 ± 1.1 MBq, 274 ± 43 MBq/kg), CHO-3013 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 150 ± 47 MBq/kg), Caki-1 xenografted NMRI-Foxn1 nu/nu mice (3.8 ± 1.2 MBq, 152 ± 47 MBq/kg) and Renca grafted BALB/cJ mice (2.5 ± 0.9 MBq, 99 ± 37 MBq/kg). C: Flow cytometry results obtained on freshly dissociated Caki-1 and Renca cells stained using 100 nM Cy5-MQ232 (total signal, red curve) or 100 nM Cy5-MQ232 in presence of 30 µM of MQ232 (non-specific signal, blue curve). Light gathered at 680 nm, 30,000 events per acquisition, two acquisitions per independent experiment, n = 3). D: Quantification results of AVPR2 -directed RT-qPCR performed on Caki-1 and Renca cells and tumors resulting from their implantation. Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney. E: Quantification results of AVPR2 -directed RT-qPCR performed on 8 ccRCC and mccRCC biopsies (P1 to P8). Three samples came from low grade primary tumors (grade ≤ 2) (P1 to P3, in green), two from high grade primary tumors (grade 3 or 4) (P4 and P5, in blue), and three from distant metastases (P6 to P8, in purple). Results are shown as fold changes between the expression in the investigated samples and the expression in the positive reference, healthy human total kidney.

Article Snippet: Caki-1 cells (human ccRCC, HTB-46, ATCC, USA) were cultured in McCoy's 5A supplemented with 10% FBS +1% penicillin/streptomycin.

Techniques: In Vivo, Injection, Computed Tomography, Imaging, Flow Cytometry, Staining, Quantitative RT-PCR, Expressing

Journal: Oncology Reports

Article Title: GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling

doi: 10.3892/or.2023.8486

Figure Lengend Snippet: GBP2 regulates PD-L1 expression at mRNA and protein levels. (A) GBP2 expression in HK-2, Caki-1, Caki-2, 786-O, and ACHN was detected using western blotting. (B) PD-L1 and GBP2 mRNA expression were detected by RT-qPCR with shNS and shGBP2#1 in Caki-1 and 786-O cell lines. (C) PD-L1 and GBP2 mRNA expression were detected by RT-qPCR with vector and GBP2 OE in Caki-1 and 786-O. (D) PD-L1 and GBP2 protein expression were detected by western blotting with shNS and shGBP2#1 in Caki-1 and 786-O cell lines. (E) PD-L1 and GBP2 protein levels were detected by western blotting with vector and GBP2 OE in Caki-1 and 786-O cell lines. Data are expressed as the mean ± SD of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. GBP 2, guanylate-binding protein 2; PD-L1, programmed death-ligand 1; RT-qPCR, reverse transcription-quantitative PCR; OE, overexpression; sh-, short hairpin.

Article Snippet: A human proximal tubular epithelial cell line (HK2), along with human Caki-2, and Caki-1 cell lines were purchased from iCell Bioscience, Inc.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Binding Assay, Real-time Polymerase Chain Reaction, Over Expression

Journal: Oxidative Medicine and Cellular Longevity

Article Title: A Decade of Research on Coffee as an Anticarcinogenic Beverage

doi: 10.1155/2021/4420479

Figure Lengend Snippet: In vitro studies in cell lines. Effects of different types of coffee on a broad spectrum of human cancer cell lines.

Article Snippet: Makino 2021 [ ] , ACHN Caki-1 , Kidney , Arabica or Robusta Coffee Japan , Roasted , Uninformed , Antiproliferative, antimigratory, apoptosis.

Techniques: In Vitro, Antioxidant Activity Assay, Activity Assay