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Image Search Results
Journal: Journal of Translational Medicine
Article Title: CCL5 promotes the epithelial-mesenchymal transition of circulating tumor cells in renal cancer
doi: 10.1186/s12967-024-05297-2
Figure Lengend Snippet: The effect of CCL5 on the phenotype and molecular aspects of CAKI-2 cells. A Transwell assays evaluating the effect of CCL5 on the migration and invasion capabilities of CAKI-2 cells. B Above: Quantitative analysis of migrated cell numbers in CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. Below: Quantitative analysis of invasive cell numbers in CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. C Colony formation assays assessing the impact of CCL5 on the cloning efficiency of CAKI-2 cells. D Quantitative analysis of colony numbers in CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. E Cell proliferation assays for CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. F Flow cytometry analysis of the effect of CCL5 on the cell cycle of CAKI-2 cells. G Quantitative analysis of cell cycle phase distribution changes in CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. H Flow cytometry analysis of CCL5's effect on apoptosis in CAKI-2 cells. I Quantitative analysis of apoptosis ratios in CAKI-2-CCL5-Con and CAKI-2-CCL5-shRNA groups. J RT-qPCR analysis of the effect of CCL5 on EMT-related markers in CAKI-2 cells. K Western blot analysis of the effect of CCL5 on the expression of EMT-related markers and smad2/3 molecules in CAKI-2 cells. L Immunofluorescence assay to detect the impact of CCL5 on E-cadherin expression in CAKI-2 cells. Experiments involving quantitative analysis were repeated at least three times, with statistical analysis conducted using the t-test
Article Snippet: The renal cancer cell lines, 786-O and
Techniques: Migration, shRNA, Clone Assay, Flow Cytometry, Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence