caiii Search Results


car3 rabbit proteintech 15197 1 ap  (Proteintech)
93
Proteintech car3 rabbit proteintech 15197 1 ap
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Car3 Rabbit Proteintech 15197 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcmv ca3  (OriGene)
92
OriGene pcmv ca3
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Pcmv Ca3, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ca3/carbonic anhydrase iii mouse monoclonal antibody (anti-caiii ab)  (Funakoshi ltd)
90
Funakoshi ltd anti-ca3/carbonic anhydrase iii mouse monoclonal antibody (anti-caiii ab)
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Anti Ca3/Carbonic Anhydrase Iii Mouse Monoclonal Antibody (Anti Caiii Ab), supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral vectors targeting caiii sequence (shcaiii) and the short hairpin rna  (Ribobio co)
90
Ribobio co lentiviral vectors targeting caiii sequence (shcaiii) and the short hairpin rna
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Lentiviral Vectors Targeting Caiii Sequence (Shcaiii) And The Short Hairpin Rna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody mouse monoclonal anti-caiii  (Abnova)
90
Abnova antibody mouse monoclonal anti-caiii
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Antibody Mouse Monoclonal Anti Caiii, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sh-caiii  (Shanghai GenePharma)
90
Shanghai GenePharma sh-caiii
Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, <t>CAR3,</t> or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.
Sh Caiii, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, CAR3, or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 3. Distribution of mouse bladder Pdgfra/PDGFRA þ fibroblasts. A: expression of Pdgfra in the bladder wall of PdgfraH2B-EGFP reporter mice as revealed by confocal microscopy. Tissue was counterstained with phalloidin to reveal the actin cytoskeleton (white) and DAPI (blue) to reveal cell nuclei. Actin was associated with the cortical cytoskeleton of most cells but strongly labeled the actin filaments that abound in the cytoplasm of smooth muscle cells. As expected for a nucleus-localized reporter, the H2B-enhanced green fluorescent protein (EGFP) signal overlapped completely with the DAPI sig- nal (see insets for example of the DAPI signal alone and EGFP signal alone). Representative confocal micrographs from two mice are shown. B: bladder tissue from PdgfraH2B-EGFP reporter mice was counterstained with antibodies to PDGFRA, CAR3, or CD34. Representative confocal micrographs from two mice are shown. Examples of H2B-EGFP þ nuclei that were also positive for PDGFRA, CAR3, or CD34 are marked with yellow arrows. In the top images, a small number of cells were found that were PDGFRAþ but H2B-EGFP– (nuclei marked with orange arrows) or were H2B-EGFPþ but PDGFRA– (nuclei labeled with cyan arrows). C: PDGFRAþ cells (green) were found in the following regions: the suburothelial region (SU); in the subja- cent outer lamina propria (OLP), which forms the core of the rougae (Ru) and extends below suburothelial cells in the unfolded regions; within the peri- mysial connective tissue in the intermuscular region (IM); and in the serosa (Se), where a single layer of cells was sandwiched between the smooth muscle and mesothelial cell (MC) layer. The tissue was counterstained with DAPI (blue) and phalloidin (white; bottom images). The boxed regions in the top left images are magnified in the adjacent images. Representative confocal micrographs from four experiments (each with 2–3 mice) are shown.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Expressing, Confocal Microscopy, Labeling

Figure 6. PDGFRAþ cells express Col1a2. A: expression of Col1a2-driven mG (mem- brane-bound enhanced green fluorescent protein) in tamoxifen-treated experimental Col1a2Cre-ERT þ /–;RosamT/mG þ /– or control Col1a2Cre-ERT–/–;RosamT/mG þ /– mice. B: coexpression of mG in the PDGFRAþ, CAR3 þ, or CD34þ cells of the indicated regions of the bladder wall [suburothelial (SU), outer lamina propria (OLP), intermus- cular (IM), and serosal (Se)]. Examples of cells with visible nuclei coexpressing these markers are marked with yellow arrows. Coexpression of MYH11 and mG was also assessed in the bottom right images. Examples of smooth muscle cells expressing mG are indicated by white arrowheads. C: use of fluorescent in situ hybridization with immunodetection (FISHID) to assess coexpression of PDGFRA, Pdgfra, and Col1a2 in the indicated regions of the bladder wall. Examples of cells (i.e., nuclei) that expressed all three markers are labeled with white arrowheads; cells that were PDGFRAþ;Pdgfraþ are marked with green arrowheads. The image marked “Control” was performed in the absence of anti- PDGFRA antibody and using the three-plex negative control probe against Bacillus sub- tilis dapB. All images are confocal micro- graphs and representative of images taken from two separate experiments each using two mice. BV, blood vessels; MC, mesothe- lial cells; Ut, urothelium.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 6. PDGFRAþ cells express Col1a2. A: expression of Col1a2-driven mG (mem- brane-bound enhanced green fluorescent protein) in tamoxifen-treated experimental Col1a2Cre-ERT þ /–;RosamT/mG þ /– or control Col1a2Cre-ERT–/–;RosamT/mG þ /– mice. B: coexpression of mG in the PDGFRAþ, CAR3 þ, or CD34þ cells of the indicated regions of the bladder wall [suburothelial (SU), outer lamina propria (OLP), intermus- cular (IM), and serosal (Se)]. Examples of cells with visible nuclei coexpressing these markers are marked with yellow arrows. Coexpression of MYH11 and mG was also assessed in the bottom right images. Examples of smooth muscle cells expressing mG are indicated by white arrowheads. C: use of fluorescent in situ hybridization with immunodetection (FISHID) to assess coexpression of PDGFRA, Pdgfra, and Col1a2 in the indicated regions of the bladder wall. Examples of cells (i.e., nuclei) that expressed all three markers are labeled with white arrowheads; cells that were PDGFRAþ;Pdgfraþ are marked with green arrowheads. The image marked “Control” was performed in the absence of anti- PDGFRA antibody and using the three-plex negative control probe against Bacillus sub- tilis dapB. All images are confocal micro- graphs and representative of images taken from two separate experiments each using two mice. BV, blood vessels; MC, mesothe- lial cells; Ut, urothelium.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Expressing, Control, In Situ Hybridization, Immunodetection, Labeling, Negative Control

Figure 9. PDGFRAþ suburothelial fibroblasts express ACTA2, CAR3, and TNC. A: ACTA2 was expressed by smooth muscle cells (SMC) in the muscularis externa and blood vessels (BV) within the lamina propria. A weaker, but reproducible, ACTA2 signal was also observed in suburo- thelial fibroblasts, subjacent to the urothelium (Ut). The dashed boxed region in the top right image is magnified in the bottom images. Nuclei of PDGFRA þ;ACTA2 þ cells are indicated by yellow arrows. B: expression of TNC and CAR3 by PDGFRAþ suburothelial fibroblasts. The dashed boxed region in the right image is reproduced in the left images. Cells with visible nuclei expressing all three markers are indicated by white arrows. Note that PDGFRA is a mem- brane protein, CAR3 is a cytoplasmic protein, and TNC is a secreted protein that is deposited in the space between ad- jacent fibroblasts. All images are confocal micrographs and representative of images taken from three-five separate experiments each with one-two mice.

Journal: American journal of physiology. Renal physiology

Article Title: Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA + interstitial cells are fibroblasts.

doi: 10.1152/ajprenal.00135.2022

Figure Lengend Snippet: Figure 9. PDGFRAþ suburothelial fibroblasts express ACTA2, CAR3, and TNC. A: ACTA2 was expressed by smooth muscle cells (SMC) in the muscularis externa and blood vessels (BV) within the lamina propria. A weaker, but reproducible, ACTA2 signal was also observed in suburo- thelial fibroblasts, subjacent to the urothelium (Ut). The dashed boxed region in the top right image is magnified in the bottom images. Nuclei of PDGFRA þ;ACTA2 þ cells are indicated by yellow arrows. B: expression of TNC and CAR3 by PDGFRAþ suburothelial fibroblasts. The dashed boxed region in the right image is reproduced in the left images. Cells with visible nuclei expressing all three markers are indicated by white arrows. Note that PDGFRA is a mem- brane protein, CAR3 is a cytoplasmic protein, and TNC is a secreted protein that is deposited in the space between ad- jacent fibroblasts. All images are confocal micrographs and representative of images taken from three-five separate experiments each with one-two mice.

Article Snippet: Primary antibodies used in the present study Antigen Host Species Source Catalog Number Dilution of stock ACTA2 Rabbit Proteintech 14395-1-AP 1:100 CAR3 Rabbit Proteintech 15197-1-AP 1:100 (1:100 for FISHID) CD34 Sheep R&D Systems AF6518 1:100 (1:50 for FISHID) CD34 Rat (clone MEC14.7) Invitrogen (ThermoFisher) MA1-22646 1:200 CSPG4 Rabbit Millipore Sigma AB5320 1:100 GFP Rabbit Abcam Ab6556 1:75 for EM KIT (CD117) Rat (clone ACK2) Stemcell 60034.1 1:100 KRT20 Rabbit Abcam ab53120 1:100 KRT5 Chicken BioLegend 905901 1:400 LY6A Rat BioLegend 108101 1:100 MYH11 Rabbit Proteintech 21404-1-AP 1:100 (1:100 for FISHID) PDGFRA Goat R&D Systems AF1062 1:100 (1:50 for FISHID) Preferred antibody used in most experiments PECAM1 (CD31) Rat (clone 390) Millipore Sigma CBL1337-I 1:100 PI16 Goat R&D Systems AF4929-SP 1:100 PI16 Rabbit US Biological 141796 1:100 PTPRC (CD45) Rat (clone IBL-3/16) Bio-Rad MCA1388 1:100 TNC Rat (clone no. 578) R&D Systems MAB2138 1:400 UCHL1 (PGP9.5) Rabbit Genetex GTX109637 1:100 VIM Rat BioLegend 699301 1:100 FISHID, fluorescent in situ hybridization with immunodetection.

Techniques: Expressing