Journal: Nature Communications

Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis

doi: 10.1038/s41467-026-69899-8

Figure Lengend Snippet: a Ribosome binding assay assessing eIF6 association with ribosome pools. Cytoplasmic lysates were fractionated into free (F1) and ribosome-associated (F2) portions via 30% sucrose cushion ultracentrifugation. Immunoblots of eIF6, RPL7, RPS15 and β-tubulin are shown. Representative of two biologically independent experiments were performed with similar results. Created in BioRender. Shen, S. (2026) https://BioRender.com/5p1ea8h . b Volcano plot of Pearson correlations between eIF6 expression and gene essentiality across ~1100 solid cancer lines from DepMap. Representative correlations for CD104 are shown for all solid tumours and SCLC lines. Two-sided p -values were computed. c Direct interaction network of eIF6 from BioGRID. d RT-qPCR of CD104 in indicated lines ( n = 3 biological replicates). Statistical significance: two-sided unpaired Wilcoxon rank-sum test. e Co-IP with anti-FAK showing interactions between CD104, eIF6, ERK1/2, and FAK in non-NE states. IgG2a as Co-IP control; β-tubulin and IgG heavy chain for input and IP loading controls. The samples derive from the same experiment, but different gels for FAK, CD104, IgG and β-tubulin, another for p-ERK, ERK and eIF6 were processed in parallel. Similar results were observed in n = 3 biologically independent experiments. PLA using anti-FAK and anti-eIF6 ( f ) or anti-CD104 ( g ) antibodies. Red fluorescent signals indicate complexes; scale bars, 10 μm. Protocol validated in three biologically independent experiments. Cell segmentation and spot quantification by CellProfiler. Created in BioRender. Shen, S. (2026) https://BioRender.com/5p1ea8h . Single-cell quantification of eIF6-FAK ( h ) and eIF6-CD104 ( i ) PLA after eIF6 knockdown. Each dot represents a single cell from one representative experiment; two additional biologically independent experiments showed similar trends. h : 490 (H69), 197 (H69M sh_NTC), 185 (H69M sh_eIF6); i : 472 (H69), 291 (H69M sh_NTC), 210 (H69M sh_eIF6). Two-sided unpaired Wilcoxon rank-sum test. j Co-IP of CD104-FAK in H69M cells with eIF6 knockdown. β-tubulin and IgG heavy chain as input/IP loading controls. The samples derive from the same experiment, but different gels for FAK, CD104, IgG, another for p-ERK, ERK, eIF6 and actin were processed in parallel. Similar results were observed in n = 3 biologically independent experiments.

Article Snippet: Briefly, slides were blocked with blocking solution for 30 minutes at RT and incubated with rabbit anti-eIF6 (Proteintech #10291-1-AP, 1:100) and mouse anti-FAK (Santa Cruz #sc1688 1:100) or rabbit anti-eIF6 and mouse anti-CD104 (R&D systems, #MAB4060, 1:100 from 500 μg ml -1 stock) at 4 °C overnight.

Techniques: Binding Assay, Western Blot, Expressing, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Control, Single Cell, Knockdown

Journal: Scientific Reports

Article Title: The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach

doi: 10.1038/srep09167

Figure Lengend Snippet: Information of primary antibody used in this study

Article Snippet: Glypican-4 (MAFB4044) , Creative Biomart (CAB-244MH) , Fix and Perm (Medium A) 15 minutes, room temp. , 10 μg/mL.

Techniques: Concentration Assay

Journal: Current opinion in HIV and AIDS

Article Title: The Promise and Pitfalls of Long Acting Injectable Agents for HIV Prevention

doi: 10.1097/COH.0000000000000219

Figure Lengend Snippet: Comparison of agents in advanced clinical development.

Article Snippet: Cabotegravir Cabotegravir (CAB, formerly GSK 1265744, ViiV Healthcare) is a novel investigational strand-transfer integrase inhibitor.

Techniques: Comparison, Reverse Transcription, Clinical Proteomics, Infection