Journal: RSC Advances

Article Title: An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor †

doi: 10.1039/d4ra02051k

Figure Lengend Snippet: Selectivity studies using a competitive method between the target molecule and the interfering species. The interfering species studied were CEA, CA 125, and urea.

Article Snippet: Potassium hexacyano trihydrate (K 4 Fe(CN) 6 ·3H 2 O), potassium hexacyano dihydrate (K 3 Fe(CN) 6 ), sodium phosphate dibasic dihydrate (Na 2 HPO 4 ·2H 2 O) and sodium dihydrogen phosphate dihydrate (NaH 2 PO 4 ·2H 2 O) were purchased from Riedel de Haën; fetal bovine serum (FBS) was purchased from Alfa Aesar; sulphuric acid (H 2 SO 4 ) was obtained from BDH; urea was from Fagron; proteinase K and (3-acrylamidopropyl)trimethylammonium chloride (solution 75 wt% in H 2 O) (AMPTMA) was acquired from Sigma-Aldrich; carcinoembryonic antigen (CEA) was obtained from EastCostBio; Cancer Antigen 125 (CA-125) was from Hytest; CA 15-3 from host human (reference MBS536585) was purchased from MyBioSource.

Techniques:

Journal: The Analyst

Article Title: Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

doi: 10.1039/c3an00668a

Figure Lengend Snippet: Fig. 1 Schematic of the sandwich immunoassay for CA125 (mAb-capture antibody; Ab–AP – alkaline phosphatase labeled detection antibody; antigen-CA125).

Article Snippet: Mouse anti-mouse CA125 IgG1, mouse anti-mouse CA125 detection antibody IgG1, human CA125 antigen and alkaline phosphatase kit were purchased from Genway Biotech (San Deigo, CA).

Techniques: Labeling

Journal: The Analyst

Article Title: Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

doi: 10.1039/c3an00668a

Figure Lengend Snippet: Fig. 2 Optimization of (A) capture antibody (1, 2, 10, and 20 mg mL1) using 1000 U mL1 CA125 antigen and 2 mg mL1 Ab–AP; and (B) Ab–AP (1, 5, 25, 50 and 100 mg mL1) with 1000 U mL1 using 2 mg mL1 capture antibody.

Article Snippet: Mouse anti-mouse CA125 IgG1, mouse anti-mouse CA125 detection antibody IgG1, human CA125 antigen and alkaline phosphatase kit were purchased from Genway Biotech (San Deigo, CA).

Techniques:

Journal: The Analyst

Article Title: Dual detection of cancer biomarker CA125 using absorbance and electrochemical methods

doi: 10.1039/c3an00668a

Figure Lengend Snippet: Fig. 5 Standard calibration curve using the CA125 antigen with 2 mg mL1 mAb CA125, 5 mg mL1 Ab–AP. (A) Absorbance based detection with 50 mL of 4 M PNPP in 0.1 M Tris, pH 9.0 after 30 min of incubation; (B) NEA-based detection using 50 mL of 4 M PAPP in 0.1 M Tris, pH 9.0 after 30 min of incubation.

Article Snippet: Mouse anti-mouse CA125 IgG1, mouse anti-mouse CA125 detection antibody IgG1, human CA125 antigen and alkaline phosphatase kit were purchased from Genway Biotech (San Deigo, CA).

Techniques: Incubation

Journal: Biosensors

Article Title: Highly Stable InSe-FET Biosensor for Ultra-Sensitive Detection of Breast Cancer Biomarker CA125.

doi: 10.3390/bios13020193

Figure Lengend Snippet: Figure 1. (a) Schematic of the InSe-FET biosensor for biomarker CA125 detection. (b) High-resolution transmission electron microscopy of InSe. (c) Thickness of typical InSe films used in FET determined by AFM. (d,e) Raman spectra and photoluminescence spectra of transferred InSe flakes.

Article Snippet: Capture antibodies, recombinant proteins, and detection antibodies, CA125 and CA199, were purchased from Fitzgerald (America).

Techniques: Biomarker Discovery, Transmission Assay, Electron Microscopy

Journal: Biosensors

Article Title: Highly Stable InSe-FET Biosensor for Ultra-Sensitive Detection of Breast Cancer Biomarker CA125.

doi: 10.3390/bios13020193

Figure Lengend Snippet: Figure 5. (a) Schematic diagram of InSe-FET biosensor for biomarker detection. (b) Transfer charac- teristic responses to target CA125 antigen in different concentrations. (c) Quantitative relationship between threshold voltage shift and antigen CA125 concentration. (d) Transfer characteristics re- sponses to the non-target antigen CA199, and (e) threshold voltage shift corresponding to different concentrations of antigen CA199. (f) Comparison of the results of antigen CA125 detection by InSe- FET biosensor and Cobas Roche electroluminescence assay on serum samples from breast cancer patients and healthy individuals. P1~3 are serum samples from three breast cancer patients, and H1~3 are serum samples from three healthy people. (g) Correlation of the results of the proposed InSe-FET biosensor with those of the Cobas e601 electroluminescence device.

Article Snippet: Capture antibodies, recombinant proteins, and detection antibodies, CA125 and CA199, were purchased from Fitzgerald (America).

Techniques: Biomarker Discovery, Concentration Assay, Comparison

Journal: Indian Journal of Ophthalmology

Article Title: Modulation of mucin secretion using combined polyethylene glycol–propylene glycol topical formulation in a hyperosmotic stress-based explant model

doi: 10.4103/ijo.ijo_2855_22

Figure Lengend Snippet: Figure 1: Effect of PEG–PG on NFAT5, MUC5AC, and MUC16 gene expression in the presence or absence of hyperosmotic stress in ex vivo CR culture. Bar graph indicates the mean mRNA expression levels of NFAT5 (a), MUC5AC (b), and MUC16 (c) normalized to the expression of β‑actin (housekeeping gene) in CRs, following exposure to hyperosmotic stress (+200 mOsm) with or without PEG–PG for 48 h. The treatment categories include control (without hyperosmotic stress and PEG–PG), CR under hyperosmotic stress (+200 mOsm), and CR under hyperosmotic stress in the presence of PEG–PG (1% and 2%) (+200 mOsm + PEG–PG). The bar graph indicates mean ± SD from three individual CRs with two technical replicates. *P < 0.05, **P < 0.01, Mann–Whitney U test. CR = corneoscleral rim, PEG–PG = polyethylene glycol–propylene glycol, SD = standard deviation

Article Snippet: Explant culture supernatants with or without treatment (n = 5) were collected and used to estimate MUC5AC (E‐EL‐H2279; Elabscience, Houston, TX, USA) and MUC16 (E‐EL‐H0636, Elabscience) according to the manufacturers’ protocols.

Techniques: Gene Expression, Ex Vivo, Expressing, Control, MANN-WHITNEY, Standard Deviation

Journal: Indian Journal of Ophthalmology

Article Title: Modulation of mucin secretion using combined polyethylene glycol–propylene glycol topical formulation in a hyperosmotic stress-based explant model

doi: 10.4103/ijo.ijo_2855_22

Figure Lengend Snippet: Figure 2: Status of secreted MUC5AC and MUC16 proteins in the CR culture following hyperosmotic stress in the presence of PEG–PG. Bar graph indicates average fold change for the secreted MUC5AC (a) and MUC16 (b) proteins normalized to their respective control. The treatment categories include control (without hyperosmotic stress and PEG–PG), CR under hyperosmotic stress (+200 mOsm), and CR under hyperosmotic stress in the presence of PEG–PG (1% and 2%) (+200 mOsm + PEG–PG). The bar graph indicates mean ± SD from supernatants collected from five individual CR explant cultures. CR = corneoscleral rim, PEG–PG = polyethylene glycol–propylene glycol, SD = standard deviation

Article Snippet: Explant culture supernatants with or without treatment (n = 5) were collected and used to estimate MUC5AC (E‐EL‐H2279; Elabscience, Houston, TX, USA) and MUC16 (E‐EL‐H0636, Elabscience) according to the manufacturers’ protocols.

Techniques: Control, Standard Deviation