Journal: OncoTargets and therapy

Article Title: Gemcitabine and carboplatin demonstrate synergistic cytotoxicity in cervical cancer cells by inhibiting DNA synthesis and increasing cell apoptosis

doi: 10.2147/OTT.S54217

Figure Lengend Snippet: Carboplatin reduced cell viability and induced DNA damage in cervical cancer cells. ( A ) SiHa and CaSki cells were treated with the indicated concentrations of carboplatin for 72 hours, and cell viability was measured by Cell Counting Kit-8 viability assay. ( B ) Carboplatin induced DNA damage in SiHa cells. SiHa cells were exposed to 20, 40, and 80 μmol/L carboplatin for 12 hours, respectively. Immunofluorescence analysis was used to detect nuclear γ-H2AX foci formation with anti-H2AX antibody (red, fluorescein isothiocyanate). Nuclei were counterstained with DAPI (blue). ( C ) γ-H2AX-positive cells were counted under a fluorescent microscope. We calculated more than 1,000 cells for each well. Quantitative data are represented the mean ± standard deviation of three individual experiments. Abbreviation: DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: SiHa and CaSki human cervical cancer cell lines (American Type Culture Collection, Manassas, VA, USA) were maintained in RPMI-1640 medium (Gibco, Carlsbad CA, USA) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, and 100 U/mL penicillin-streptomycin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Cell Counting, Viability Assay, Immunofluorescence, Microscopy, Standard Deviation

Journal: OncoTargets and therapy

Article Title: Gemcitabine and carboplatin demonstrate synergistic cytotoxicity in cervical cancer cells by inhibiting DNA synthesis and increasing cell apoptosis

doi: 10.2147/OTT.S54217

Figure Lengend Snippet: Synergistic cytotoxicity of gemcitabine combined with carboplatin in cervical cancer cell lines. ( A ) SiHa and CaSki cells were seeded into 96-well plates and treated with gemcitabine or/and carboplatin at the indicated concentrations. After 72 hours, cell viability was measured using the Cell Counting Kit-8 viability assay. The data shown represent the mean ± standard deviation (n=3). ( B ) The synergistic effect of gemcitabine combined with carboplatin was quantitatively analyzed with a Cl and expressed as log 10 (CI) versus fractional effect Where calculable, 95% confidence intervals are shown. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GEM, gemcitabine; CBDCA, carboplatin; CI, combination index; SD, standard deviation.

Article Snippet: SiHa and CaSki human cervical cancer cell lines (American Type Culture Collection, Manassas, VA, USA) were maintained in RPMI-1640 medium (Gibco, Carlsbad CA, USA) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, and 100 U/mL penicillin-streptomycin at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Cell Counting, Viability Assay, Standard Deviation