Journal: eLife

Article Title: Evaluating the transcriptional regulators of arterial gene expression via a catalogue of characterized arterial enhancers

doi: 10.7554/eLife.102440

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , ChIP DNA Clean & Concentrator kit , Zymo Research , D5205 , .

Techniques: Recombinant, Control, Multiplex Assay, TA Cloning, Software

Journal: Journal of molecular and cellular cardiology

Article Title: Mitochondrial proteome disruption in the diabetic heart through targeted epigenetic regulation at the mitochondrial heat shock protein 70 (mtHsp70) nuclear locus.

doi: 10.1016/j.yjmcc.2018.04.016

Figure Lengend Snippet: Fig. 7. MiRNA targets and CpG island methylation for HSPA9 and Hspa9. (A) ND (left, n = 6) versus T2DM (right, n = 6) miRNA expression levels predicted to target HSPA9 in human patient right atrial tissue (black bars) and control (left, n = 6) versus db/db (right, n = 6) miRNA expression levels predicted to target Hspa9 in mice whole heart (white bars). (B) CpG island methylation in the promotor region of Hspa9 in control (black, n = 6) versus db/db (grey, n = 6) mice. (C) CpG island methylation in the promotor region of HSPA9 in non-diabetic (black, n = 6) versus type 2 diabetic (grey, n = 6) human patient samples. For both human and mouse CpG methylation, 10 clones were selected from each group. (D) An overview of the process for measuring and analyzing CpG island methylation at the HSPA9 promoter region, revealing limited methylation in both the control and diabetic cohort. Values are expressed as means ± SEM; *P ≤.05 for control vs. T2DM. MiRNA analyses were normalized to U6. HSPA9 = Heat Shock Protein Family A (Hsp70) Member 9, ND = non-diabetic, T2DM = type 2 diabetes mellitus.

Article Snippet: RNA was converted to cDNA using a First-strand cDNA Synthesis kit for miRNA (Origene, Rockville, MD, Catalog #: HP100042), per manufacturer's instructions.

Techniques: Methylation, Expressing, Control, CpG Methylation Assay, Clone Assay

Journal: eLife

Article Title: MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior

doi: 10.7554/eLife.75233

Figure Lengend Snippet:

Article Snippet: Commercial assay, kit , ClarityWestern blot ECL , Bio-Rad#170–5061 , , .

Techniques: Sequencing, Control, CRISPR, Biomarker Discovery, Reverse Transcription, Expressing, Recombinant, Electroporation, Plasmid Preparation, Blocking Assay, Transmission Assay, RNA Extraction, Lysis, Transfection, Cloning, cDNA Synthesis, Software, Microscopy, Imaging, Western Blot, Quantitation Assay

Journal: eLife

Article Title: Nuclear receptor NR4A is required for patterning at the ends of the planarian anterior-posterior axis

doi: 10.7554/eLife.42015

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , Nextera XT DNA Library Preparation Kit , Illumina , FC-131–1002 , Used for single-cell RNA sequencing DNA library construction.

Techniques: Clone Assay, Staining, RNA Sequencing, DNA Library Preparation, In Situ, TUNEL Assay, Labeling, Software, Quantitative Proteomics, Sequencing

Journal: eLife

Article Title: Binary outcomes of enhancer activity underlie stable random monoallelic expression

doi: 10.7554/eLife.74204

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , NEBNext Ultra II DNA Library Prep Kit for Illumina , New England Biolabs , Cat # E7645S , .

Techniques: Generated, Microinjection, Electroporation, CRISPR, Purification, cDNA Synthesis, Cell Isolation, Multiplex Assay, TA Cloning, Subcloning, Staining, Flow Cytometry, Recombinant, Cell Culture, Software

Journal: Cell reports

Article Title: Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

doi: 10.1016/j.celrep.2022.110553

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: QIAmo Fast DNA Stool kit , Qiagen , 51604.

Techniques: Control, Plasmid Preparation, Recombinant, Cell Isolation, cDNA Synthesis, SYBR Green Assay, Staining, Enzyme-linked Immunosorbent Assay, Cloning, Library Quantification, Software