c8 d1a Search Results


mouse astrocyte cell line c8 d1a crl 2541  (ATCC)
96
ATCC mouse astrocyte cell line c8 d1a crl 2541
Mouse Astrocyte Cell Line C8 D1a Crl 2541, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse astrocyte cell line c8 d1a crl 2541/product/ATCC
Average 96 stars, based on 1 article reviews
mouse astrocyte cell line c8 d1a crl 2541 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
c8-d1a cell line  (Procell Inc)
90
Procell Inc c8-d1a cell line
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
C8 D1a Cell Line, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c8-d1a cell line/product/Procell Inc
Average 90 stars, based on 1 article reviews
c8-d1a cell line - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
astrocyte cell line c8-d1a cl-0506  (Keygen Biotech)
90
Keygen Biotech astrocyte cell line c8-d1a cl-0506
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
Astrocyte Cell Line C8 D1a Cl 0506, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/astrocyte cell line c8-d1a cl-0506/product/Keygen Biotech
Average 90 stars, based on 1 article reviews
astrocyte cell line c8-d1a cl-0506 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
c8-d1a astrocyte cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures c8-d1a astrocyte cell line
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
C8 D1a Astrocyte Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c8-d1a astrocyte cell line/product/European Collection of Authenticated Cell Cultures
Average 90 stars, based on 1 article reviews
c8-d1a astrocyte cell line - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
c8 d1a cells  (Elabscience Biotechnology)
92
Elabscience Biotechnology c8 d1a cells
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
C8 D1a Cells, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c8 d1a cells/product/Elabscience Biotechnology
Average 92 stars, based on 1 article reviews
c8 d1a cells - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
mouse astrocyte cell line c8- d1a  (ScienCell)
90
ScienCell mouse astrocyte cell line c8- d1a
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
Mouse Astrocyte Cell Line C8 D1a, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse astrocyte cell line c8- d1a/product/ScienCell
Average 90 stars, based on 1 article reviews
mouse astrocyte cell line c8- d1a - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
c8-d1a cells  (Xiehe Group)
90
Xiehe Group c8-d1a cells
Mn induced cytotoxicity and disrupted α-Syn clearance in <t>C8-D1A</t> cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control
C8 D1a Cells, supplied by Xiehe Group, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c8-d1a cells/product/Xiehe Group
Average 90 stars, based on 1 article reviews
c8-d1a cells - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
c8-d1a cells  (CLS Cell Lines Service GmbH)
N/A
C8-D1A cells are derived from explant cultures of the cerebellum of an 8-day-old mouse, and are classified as an astrocyte type I cell line. The derivation of C8-D1A cells involved a rigorous multistage process, commencing
   Buy from Supplier
c8-d1a cells complete medium  (Innovative Research Inc)
N/A
C8-D1A Cells Complete Medium is a cells complete medium from Innovative Research, supplied as a ready-to-use liquid. More Details: Formulation: DMEM + 10% FBS + 1% P/S Bacterial detection: Negative Fungal detection: Negative Mycoplasma detection:
   Buy from Supplier

Image Search Results


Mn induced cytotoxicity and disrupted α-Syn clearance in C8-D1A cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control

Journal: Cell Biology and Toxicology

Article Title: Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction

doi: 10.1007/s10565-025-09989-3

Figure Lengend Snippet: Mn induced cytotoxicity and disrupted α-Syn clearance in C8-D1A cells. A : Dose-dependent change of cell viability measured at 24 h after treatment with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). B - D : The mRNA levels of Gfap , Pak1 and Rac1 were determined after different concentrations of Mn treatment at 24 h. E – F : The protein level and semi-quantitative analysis of Serpina3n after Mn exposure for 24 h. G : The mRNA expression of Serpina3n in WT and Serpina3n KO C8-D1A cells treated with indicated concentrations of Mn for 24 h. H - I : Immunofluorescence co-localization analysis of intracellular α-Syn (red) with GFAP (green) after Mn exposure (50 μmol/L) in the absence or presence of 0.8 μg/mL α-Syn for 24 h in WT and Serpina3n KO C8-D1A cells. Scale bar: 50 μm. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control

Article Snippet: The C8-D1A cell line was purchased from Procell Company (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Control

Mn impaired lysosome function in C8-D1A cells. C8-D1A cells were treated with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). A : Lysosomal acidic environment was measured by LysoSensor DND-189 fluorescence intensity. B - C : CTSB and CTSD activities were detected using corresponding assay kits. D - E : Changes of mRNA expression of Gba and Gusb . F : The mRNA levels of H + transporting ATPase in C8-D1A cells after Mn exposure (50 μmol/L) for 24 h. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control

Journal: Cell Biology and Toxicology

Article Title: Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction

doi: 10.1007/s10565-025-09989-3

Figure Lengend Snippet: Mn impaired lysosome function in C8-D1A cells. C8-D1A cells were treated with different concentrations of MnCl 2 (0, 12.5, 25, 50 μmol/L). A : Lysosomal acidic environment was measured by LysoSensor DND-189 fluorescence intensity. B - C : CTSB and CTSD activities were detected using corresponding assay kits. D - E : Changes of mRNA expression of Gba and Gusb . F : The mRNA levels of H + transporting ATPase in C8-D1A cells after Mn exposure (50 μmol/L) for 24 h. The values are presented as means ± SEM. Each experiment was employed at least three times. *p < 0.05 , **p < 0.01, ***p < 0.001 vs control

Article Snippet: The C8-D1A cell line was purchased from Procell Company (Wuhan, China).

Techniques: Fluorescence, Expressing, Control

Serpina3n KO alleviated Mn-induced reduction of Lamp1 and Lamp2 in astrocytes and mouse midbrain. A : The protein level of Lamp1 ( n = 5) and Lamp2 ( n = 4) in WT and Serpina3n KO C8-D1A cells treated with 50 μM MnCl 2 for 24 h. B - C : Semi-quantitative analysis. D : The protein level of Lamp1 and Lamp2 in mice midbrain after Mn exposure for 6 weeks. E – F : Semi-quantitative analysis ( n = 6). G: Immunofluorescent co-localization analysis of Lamp1 (green) with α-Syn (red) and semi-quantitative analysis in the striatum of mice. Scale bar: 50 μm. H: 8 non-overlapping areas of the midbrain of 4 mice (sex ratio 1:1) were assigned for semi-quantitative analysis. The values are presented as means ± SEM. * p < 0.05 , ** p < 0.01

Journal: Cell Biology and Toxicology

Article Title: Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction

doi: 10.1007/s10565-025-09989-3

Figure Lengend Snippet: Serpina3n KO alleviated Mn-induced reduction of Lamp1 and Lamp2 in astrocytes and mouse midbrain. A : The protein level of Lamp1 ( n = 5) and Lamp2 ( n = 4) in WT and Serpina3n KO C8-D1A cells treated with 50 μM MnCl 2 for 24 h. B - C : Semi-quantitative analysis. D : The protein level of Lamp1 and Lamp2 in mice midbrain after Mn exposure for 6 weeks. E – F : Semi-quantitative analysis ( n = 6). G: Immunofluorescent co-localization analysis of Lamp1 (green) with α-Syn (red) and semi-quantitative analysis in the striatum of mice. Scale bar: 50 μm. H: 8 non-overlapping areas of the midbrain of 4 mice (sex ratio 1:1) were assigned for semi-quantitative analysis. The values are presented as means ± SEM. * p < 0.05 , ** p < 0.01

Article Snippet: The C8-D1A cell line was purchased from Procell Company (Wuhan, China).

Techniques:

TFEB-v/p-ATPases signaling was involved in the Serpina3n-mediated counteraction against Mn-induced lysosome dysfunction in C8-D1A cells. Wild type and Serpina3n KO C8-D1A cells were treated with 50 μM MnCl 2 for 24 h. A - B : Immunofluorescence analysis of TFEB staining and its semi-quantification. Scale bar: 5 μm. C : Changes in mRNA expressions of TFEB target genes. D : Changes in mRNA expressions of v/p-ATPases genes. E : LysoSensor DND-189 fluorescence intensity. F - G : CTSB ( F ) and CTSD ( G ) activities. All experiments were repeated at least 3 times. The values are presented as means ± SEM. * p < 0.05 , ** p < 0.01

Journal: Cell Biology and Toxicology

Article Title: Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction

doi: 10.1007/s10565-025-09989-3

Figure Lengend Snippet: TFEB-v/p-ATPases signaling was involved in the Serpina3n-mediated counteraction against Mn-induced lysosome dysfunction in C8-D1A cells. Wild type and Serpina3n KO C8-D1A cells were treated with 50 μM MnCl 2 for 24 h. A - B : Immunofluorescence analysis of TFEB staining and its semi-quantification. Scale bar: 5 μm. C : Changes in mRNA expressions of TFEB target genes. D : Changes in mRNA expressions of v/p-ATPases genes. E : LysoSensor DND-189 fluorescence intensity. F - G : CTSB ( F ) and CTSD ( G ) activities. All experiments were repeated at least 3 times. The values are presented as means ± SEM. * p < 0.05 , ** p < 0.01

Article Snippet: The C8-D1A cell line was purchased from Procell Company (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence