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Genecopoeia
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Revvity
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Vitagen Inc
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Corning Life Sciences
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Vital Therapies Inc
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Stem Cell Innovations Inc
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Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of
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This product is a cell line stably expressing the CRISPR Cas9 nuclease. This cell line also expresses the hygromycin resistance gene. In combination with separately transfected or transduced single guide RNAs (sgRNAs), this cell line
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This product is a labeled stable pool in the designated cell type. This stable cell line expresses eGFP. This cell line can be used in vitro for cancer cell line research or in vivo to
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Image Search Results
Journal: Frontiers in Immunology
Article Title: TLQP-21 is a low potency partial C3aR activator on human primary macrophages
doi: 10.3389/fimmu.2023.1086673
Figure Lengend Snippet: hTLQP-2 and mTLQP-21 activate ERK signalling in CHO-K1 and CHO-C3aR cells. hTLQP-21, mTLQP-21 and plasma-derived human C3a were tested in (A) non-transfected CHO-K1 or (B) CHO cells stably expressing human C3aR. CHO cells were serum-starved overnight and then stimulated with various ligands for 10 min before being lysed. The phospho-ERK1/2 content in the lysate was measured and expressed as fold-baseline before being combined. The maximum relative pERK1/2 activity induced by each ligand is shown in (C) . Data represent mean ± S.E.M. of triplicate measurements from 3-4 independent experiments (n = 3-4). Two-way ANOVA with Dunnett’s post hoc analysis. * P < 0.05, *** P < 0.001, **** P < 0.0001. Ligand treated versus medium treated cells for each cell line.
Article Snippet: Non-transfected CHO-K1 cells or CHO cells stably expressing the
Techniques: Clinical Proteomics, Derivative Assay, Transfection, Stable Transfection, Expressing, Activity Assay
Journal: Frontiers in Immunology
Article Title: TLQP-21 is a low potency partial C3aR activator on human primary macrophages
doi: 10.3389/fimmu.2023.1086673
Figure Lengend Snippet: Summary of potencies and activities of TLQP-21 tested on CHO-C3aR, HMDM and BMDM.
Article Snippet: Non-transfected CHO-K1 cells or CHO cells stably expressing the
Techniques: Activity Assay
Journal: Frontiers in Immunology
Article Title: TLQP-21 is a low potency partial C3aR activator on human primary macrophages
doi: 10.3389/fimmu.2023.1086673
Figure Lengend Snippet: TLQP-21 triggers ERK signalling through C3aR in murine bone marrow-derive macrophages. BMDMs (90,000/well) from wildtype mice (A) or with C3aR knockout mice (B) , were serum-starved overnight and then stimulated with respective ligands at the indicated concentrations for 10 min. The phospho-ERK1/2 content in the cell lysate was measured and normalised to the medium only-treated levels before being combined. Data represent mean ± S.E.M. of triplicate measurements using cells from 3 mice (n = 3).
Article Snippet: Non-transfected CHO-K1 cells or CHO cells stably expressing the
Techniques: Knock-Out