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Image Search Results
Journal: Microorganisms
Article Title: Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription
doi: 10.3390/microorganisms14030564
Figure Lengend Snippet: Expression analysis of 44 RNA polymerase (RNAP) variants. All RNAP variants were expressed in E. coli BL21(DE3), and crude lysate samples were analyzed via SDS-PAGE. An empty vector control (IPTG-induced cells harboring an empty pET28a vector) was used for the gels to determine host-derived background protein bands. The gel images show sections of gels at sizes relevant for the respective protein. The samples were collected after four hours of induction with 0.5 mM IPTG. The size of all proteins is given in kilodaltons (kDa) and indicated below each SDS gel.
Article Snippet: BL21(
Techniques: Expressing, SDS Page, Plasmid Preparation, Control, Derivative Assay, SDS-Gel