Journal: Analytical Chemistry

Article Title: Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection

doi: 10.1021/acs.analchem.3c03691

Figure Lengend Snippet: Dose–response curves obtained for the heterogeneous sandwich CRP immunoassays using C153-loaded PSP. The measured emission intensities are normalized to the respective background signal for each assay. These intensities were recorded by measuring (a) the PL intensity of the dye-loaded PSP or (b) the PL signals of the dye molecules released from the particles triggered by extraction with EtOH using 0.1 mg/mL PSP in each experiment. The performance of the differently sized C153-loaded PSP reporters with a PSP concentration of 0.1 mg/mL and different concentrations of 100 nm sized PSP, stained with 10 mM C153 in the CRP immunoassay measured under otherwise identical conditions, is shown in (c) and (d), respectively.

Article Snippet: Coumarin 153 (C153) was obtained from Radiant Dyes Laser GmbH.

Techniques: Extraction, Concentration Assay, Staining

Journal: Analytical Chemistry

Article Title: Exploring Simple Particle-Based Signal Amplification Strategies in a Heterogeneous Sandwich Immunoassay with Optical Detection

doi: 10.1021/acs.analchem.3c03691

Figure Lengend Snippet: Overview of the EC50 Values, Limits of Detection (LOD), Measurement Range, and Relative Dynamic Range (RDR) Obtained for the Different Detection Schemes Using Photoluminescence (PL), Chemiluminescence (CL), or Absorbance (Abs) Measurements

Article Snippet: Coumarin 153 (C153) was obtained from Radiant Dyes Laser GmbH.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Coumarin 153 (C153) structure

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: C153 absorption spectra at room temperature ( a ) and at 233 K ( b ) in: ClP ( solid ), ClH ( long dash ), PPN ( short dash ), EtOH ( dot–dash ) and TFEtOH ( dot )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Changes in absorbance of C153 in ClH induced by decrease in temperature: 323 K→313 K ( solid line ), 263 K→253 K ( dot ) and 193 K→183 K ( dash )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: C153 steady-state absorption spectra maximum positions ( ν p ) vs temperature in: ClH ( filled squares ), ClP ( filled triangles ), PPN ( empty diamonds ), EtOH ( empty triangles ) and TFEtOH ( filled circles ). Lines correspond to theoretically determined positions

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Temperature dependencies of the fluorescence quantum yield, ϕ F , of C153 in ClH ( a ), PPN ( b ), EtOH ( c ) and TFEtOH ( d )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques: Fluorescence

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Temperature dependencies of the fluorescence decay time for C153 in ClH ( a ), PPN ( b ), EtOH ( c ) and TFEtOH ( d ). In the alcohols the dominant long component time ( filled circles ) of the double-exponential decay function fitted to the experimental decays is presented along with the single exponential decay time for TFEtOH ( empty circles )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques: Fluorescence

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Temperature dependence of fluorescence decay time of C153 in dehydrated ClH ( a )– filled circles , in ClH+water mixture at 2.2⋅10 −3 M water concentration ( a )– empty circles , in dehydrated PPN ( b )– filled circles and in improperly dehydrated PPN ( b )– empty circles

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques: Fluorescence, Concentration Assay

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Times and amplitudes of fluorescence decay components found for C153 in ClH+water mixture at 2.2 × 10 −3 M water concentration and at selected temperatures and wavelengths

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques: Fluorescence, Concentration Assay

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Decay time values, τ si , and amplitudes, b i , of the exponential decay components fitted to the solvation correlation function, C(t), determined for C153 in EtOH and TFEtOH at selected temperatures. 〈 τ s 〉 – average decay time

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Stokes shift vs temperature for C153 in: ClP ( filled circles ), ClH ( empty circles ), PPN ( filled triangles ), EtOH ( empty triangles ) and TFEtOH ( filled squares )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques:

Journal: Journal of Fluorescence

Article Title: The Influence of Temperature on C153 Steady-State Absorption and Fluorescence Kinetics in Hydrogen Bonding Solvents

doi: 10.1007/s10895-012-1109-2

Figure Lengend Snippet: Temperature dependence of: ( a ) E 00 , S 1 –S 0 energy gap for C153 in ClH ( filled circles ) and PPN ( empty circles ), \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \Delta {\overline v_{1/2}} $$\end{document} -full width at half-maximum in ClH ( filled squares ) and PPN ( empty squares ), \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ {\overline v_{\text{A}}} $$\end{document} -in ClH ( filled triangles ) and PPN ( empty triangles ); ( b ) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ {\overline \xi_{\text{A}}} $$\end{document} —Huang-Rhys vibrational coupling factor in ClH ( filled circles ) and PPN ( empty circles ) as obtained from fit of C153 emission spectra in both solvents to the model given in Eqs. ( – )

Article Snippet: C153 (Fluka) was used as received, 1-chlorohexane (Aldrich), propionitrile (Sigma-Aldrich), ethanol (Sigma-Aldrich) and tri-fluoroethanol (Sigma-Aldrich) were dehydrated using 3 Å (Merck) and 4 Å (Fluka) molecular sieves and the sample were prepared under argon atmosphere after solvent dehydration.

Techniques: