c12206 Search Results


c12206  (PromoCell)
99
PromoCell c12206
C12206, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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growth medium 2 egm 2  (PromoCell)
95
PromoCell growth medium 2 egm 2
( a ) Optimisation of 18 F-FDG labelling concentrations performed at different time-points under starvation conditions (PBS), expressed as % incorporation relative to incubation medium and washes, n = 3–4. Data presented as dose-response curves for three separate incubation time-points. ( b ) Leakage of intracellular 18 F-FDG over time at room temperature, n = 3. ( c ) Optimisation of 18 F-FLT labelling concentrations performed at different time-points under growth conditions <t>(EGM-2),</t> expressed as % incorporation relative to incubation medium and washes, n = 5. Data presented as dose-response curves for three separate incubation time-points. ( d ) Leakage of intracellular 18 F-FLT over time at room temperature, n = 4–5.
Growth Medium 2 Egm 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human umbilical vein  (PromoCell)
95
PromoCell human umbilical vein
( a ) Optimisation of 18 F-FDG labelling concentrations performed at different time-points under starvation conditions (PBS), expressed as % incorporation relative to incubation medium and washes, n = 3–4. Data presented as dose-response curves for three separate incubation time-points. ( b ) Leakage of intracellular 18 F-FDG over time at room temperature, n = 3. ( c ) Optimisation of 18 F-FLT labelling concentrations performed at different time-points under growth conditions <t>(EGM-2),</t> expressed as % incorporation relative to incubation medium and washes, n = 5. Data presented as dose-response curves for three separate incubation time-points. ( d ) Leakage of intracellular 18 F-FLT over time at room temperature, n = 4–5.
Human Umbilical Vein, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huve cells  (PromoCell)
95
PromoCell huve cells
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Huve Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cells  (PromoCell)
95
PromoCell endothelial cells
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Endothelial Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvec  (PromoCell)
95
PromoCell huvec
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Huvec, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huve cells passage 3 4  (PromoCell)
95
PromoCell huve cells passage 3 4
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Huve Cells Passage 3 4, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvec cells  (PromoCell)
95
PromoCell huvec cells
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Huvec Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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culture conditions huvecs  (PromoCell)
96
PromoCell culture conditions huvecs
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Culture Conditions Huvecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mv 2  (PromoCell)
96
PromoCell mv 2
In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, <t>HUVE,</t> F9 <t>and</t> <t>A673</t> cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.
Mv 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( a ) Optimisation of 18 F-FDG labelling concentrations performed at different time-points under starvation conditions (PBS), expressed as % incorporation relative to incubation medium and washes, n = 3–4. Data presented as dose-response curves for three separate incubation time-points. ( b ) Leakage of intracellular 18 F-FDG over time at room temperature, n = 3. ( c ) Optimisation of 18 F-FLT labelling concentrations performed at different time-points under growth conditions (EGM-2), expressed as % incorporation relative to incubation medium and washes, n = 5. Data presented as dose-response curves for three separate incubation time-points. ( d ) Leakage of intracellular 18 F-FLT over time at room temperature, n = 4–5.

Journal: Scientific Reports

Article Title: PET Cell Tracking Using 18 F-FLT is Not Limited by Local Reuptake of Free Radiotracer

doi: 10.1038/srep44233

Figure Lengend Snippet: ( a ) Optimisation of 18 F-FDG labelling concentrations performed at different time-points under starvation conditions (PBS), expressed as % incorporation relative to incubation medium and washes, n = 3–4. Data presented as dose-response curves for three separate incubation time-points. ( b ) Leakage of intracellular 18 F-FDG over time at room temperature, n = 3. ( c ) Optimisation of 18 F-FLT labelling concentrations performed at different time-points under growth conditions (EGM-2), expressed as % incorporation relative to incubation medium and washes, n = 5. Data presented as dose-response curves for three separate incubation time-points. ( d ) Leakage of intracellular 18 F-FLT over time at room temperature, n = 4–5.

Article Snippet: Pooled donor primary HUVECs (C-12208, PromoCell, Germany) were cultured in endothelial growth medium 2 (EGM-2) (PromoCell, Germany) on 0.1% gelatine (Sigma, USA)-coated culture flasks/plates and, where stated, Dulbecco’s Phosphate-Buffered Saline (DPBS) (Lonza, Switzerland) or endothelial basal medium (EBM) (PromoCell, Germany).

Techniques: Incubation

In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, HUVE, F9 and A673 cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.

Journal: British Journal of Cancer

Article Title: Clodronate-liposome-mediated depletion of tumour-associated macrophages: a new and highly effective antiangiogenic therapy approach

doi: 10.1038/sj.bjc.6603240

Figure Lengend Snippet: In vitro and in vivo effects of free and liposome encapsulated clodronate (clodrolip). ( A ) Concentration-dependent cytotoxicity of clodrolip on macrophages (isolated from Sv129 mice by peritoneal lavage) in vitro . ( B ) Cytotoxicity of clodronate or clodrolip on different cells in vitro . Macrophages, HUVE, F9 and A673 cells were cultured in the presence of 1 mg ml −1 clodronate or clodrolip for 6 h. Results are means±s.e.m. ( n =3). Statistical analysis: * P <0.05 vs untreated cells. ( C ) Selective depletion of spleen cell populations after treatment with clodronate and clodrolip. Spleen tissues obtained from immunocompetent Sv129 mice injected with PB, clodronate or with clodrolip are shown (initial dose 2 mg 20 g −1 mouse body weight, followed by 1 mg, every 4 days, i.p.). Spleens were removed and sections IHC stained for marginal zone metallophilic MOMA1 + , marginal zone ER-TR 9 + , red pulp F4/80 + , CD68 + and CD11b + macrophages, the DC subsets FDC + and CD11c + , B220 + B cells, and CD3 + T cells. Bar: 100 μ m.

Article Snippet: Murine F9 teratocarcinoma (CRL-1720), human A673 rhabdomyosarcoma (CRL-1598) were from American Type Culture Collection (ATCC) and HUVE cells from PromoCell (Heidelberg, Germany).

Techniques: In Vitro, In Vivo, Concentration Assay, Isolation, Cell Culture, Injection, Staining