Journal: STAR Protocols

Article Title: Protocol for parotidectomy and saliva analysis in mice

doi: 10.1016/j.xpro.2021.101048

Figure Lengend Snippet:

Article Snippet: Sterile Collection Swab, 20mm break point , ZYMO , Cat#: C1052-50.

Techniques: Recombinant

Journal: Frontiers in Oncology

Article Title: CD47 Enhances Cell Viability and Migration Ability but Inhibits Apoptosis in Endometrial Carcinoma Cells via the PI3K/Akt/mTOR Signaling Pathway

doi: 10.3389/fonc.2020.01525

Figure Lengend Snippet: CD47 up-regulation inhibited apoptosis in HEC-1A and Ishikawa cells. (A,B) Up-regulation of CD47 inhibited cell apoptosis in Ishikawa cell line. (C,D) CD47 overexpression inhibited cell apoptosis in HEC-1A cell line. * p < 0.05.

Article Snippet: The cell cycle of transfected HEC-1A and Ishikawa cells were analyzed using the Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's protocol.

Techniques: Over Expression

Journal: Microorganisms

Article Title: An Analysis of the Gut Microbiota and Related Metabolites following PCSK9 Inhibition in Statin-Treated Patients with Elevated Levels of Lipoprotein(a)

doi: 10.3390/microorganisms12010170

Figure Lengend Snippet: ( A – C ) Boxplots representing the concentration (log10 transformed) of total bile acids ( A ), and primary ( B ) and secondary ( C ) bile acids in fecal samples collected at baseline and on treatment. Samples from the same patient are connected using straight lines. The following primary bile acids were detected in feces: cholic acid, taurocholic acid, chenodeoxycholic acid, taurochenodeoxycholic acid and glycochenodeoxycholic acid. The following secondary bile acids were detected in feces: deoxycholic acid, lithocholic acid, ursodeoxycholic acid, taurolithocholic acid, tauroursodeoxycholic acid, taurodeoxycholic acid and glycodeoxycholic acid. For statistical inference, we used a paired t -test, and the corresponding p -values are shown in each graph. ( D ) Linear relationship between the reduction in plasma LDL-c and the concentration of fecal bile acids (log10 transformed) after alirocumab therapy. Line of best fit for the linear model is plotted with 95% confidence interval (grey shade area). Individual samples are represented with black dots. The regression coefficient, coefficient of determination and the result of the F-test for the linear model are shown on the top of the plot.

Article Snippet: For identification of the different classes of BAs, we used the following pure standards to create a mass spectral–retention time library; taurocholic acid (Santa Cruz, sc220189, Dallas, TX, USA), taurolithocholic acid (Cayman Chemicals, 17275, Ann Arbor, MI, USA), taurochenodeoxycholic acid (Steraloids, C1162-000, Newport, RI, USA), tauroursodeoxycholic acid (Steraloids, C1052-000), glycolithocholic acid (Steraloids, C1435-000), glycochenodeoxycholic acid (Steraloids, C0962-000), glycoursodeoxycholic acid (Steraloids, C1025-000), glycocholic acid (Steraloids, C1927-000), glycodeoxycholic acid (Steraloids, C1087-000), ursodeoxycholic acid (Steraloids, C1020-000), cholic acid (Sigma-Aldrich, C1129, St. Louis, MO, USA), deoxycholic acid (Sigma-Aldrich, D2510), lithocholic acid (Sigma-Aldrich, L6250), chenodeoxycholic acid (Sigma-Aldrich, C1050000).

Techniques: Concentration Assay, Transformation Assay, Clinical Proteomics