Journal: PLoS ONE

Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry

doi: 10.1371/journal.pone.0221903

Figure Lengend Snippet: A , Detection by C-PCR (conventional PCR) using RST 31/33 primers. DNA concentrations are in nanogram per microliter (ng/μl) from the serially diluted DNA extract from X . fastidiosa pure cultures. B, AmplifyRP® Accelar8™ positive samples were determined by the presence of test line with the control line (1 to 4) and negative samples were determined as the absence of test line (5 to 7). The concentration of viable cells were represented as cfu/ml; C, D, Double antibody sandwich ELISA (DAS-ELISA) samples were determined to be positive (10 5 to 10 8 ) if the absorbance at 405 nm was three times greater than the mean absorbance of control samples; E, F, Loop-mediated isothermal amplification (LAMP) successful amplification was visualized by agarose gel image and naked colorimetric view. Yellow color of the pH-sensitive dye Phenol Red with X . fastidiosa- positive samples (tubes 1 to 4) and pink with the negative samples (tubes 5 to 7) real-time polymerase chain reaction (qPCR); G Real-time PCR results. Ct = cycle threshold values.

Article Snippet: In addition, there was some minor variation between C-PCR detection levels which supports a previous report by Nissen [ ] where variation among X . fastidiosa specific C-PCR primers detection limit ranged from 2.5 pg to less than 1.0 pg DNA/ reaction, but we achieved an approximate detection limit for this assay for the primers we used in this study.

Techniques: Control, Concentration Assay, Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Amplification, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry

doi: 10.1371/journal.pone.0221903

Figure Lengend Snippet: A, Amplicons obtained by C-PCR (Rubisco as internal control gene); B,C, LAMP successful amplification of X . fastidiosa was visualized with yellow color of the pH-sensitive dye Phenol Red (tubes 1–10) or negative sample remains pink (tube 11); D, AmplifyRP® Accelar8™ positive samples were determined by the presence of test line with the control line (2 to 11) and negative samples were determined as the absence of test line (1); E, DAS-ELISA and F, Real-time PCR (log10 of the copy number of Xylella fastidiosa molecules was obtained from the regression equation y = -3.4491x + 38.833). M = 100 bp ladder marker.

Article Snippet: In addition, there was some minor variation between C-PCR detection levels which supports a previous report by Nissen [ ] where variation among X . fastidiosa specific C-PCR primers detection limit ranged from 2.5 pg to less than 1.0 pg DNA/ reaction, but we achieved an approximate detection limit for this assay for the primers we used in this study.

Techniques: Control, Amplification, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Marker

Journal: PLoS ONE

Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry

doi: 10.1371/journal.pone.0221903

Figure Lengend Snippet: Comparison between five different molecular and serological techniques for detection of Xylella fastidiosa .

Article Snippet: In addition, there was some minor variation between C-PCR detection levels which supports a previous report by Nissen [ ] where variation among X . fastidiosa specific C-PCR primers detection limit ranged from 2.5 pg to less than 1.0 pg DNA/ reaction, but we achieved an approximate detection limit for this assay for the primers we used in this study.

Techniques: Comparison, DNA Extraction, Detection Assay, Concentration Assay, Real-time Polymerase Chain Reaction