Journal: Antioxidants (Basel, Switzerland)

Article Title: The Carotenoid Diatoxanthin Modulates Inflammatory and Angiogenesis Pathways In Vitro in Prostate Cancer Cells.

doi: 10.3390/antiox12020359

Figure Lengend Snippet: Figure 2. Angiogenesis inhibition in prostate cancer cell lines. (A) Human angiogenesis array analysis of the conditional medium from PC3 cells treated with Dt, and relative expression levels of the proangiogenesis cytokines. (B) Human inflammation array analysis of the conditional medium from DU145 cells treated with Dt and relative expression levels of the inflammation cytokines. (C,D) Molecular docking diagrams of Dt binding with TLR4 (C) and FCGR1A (D). Asterisks indicate the statistically significant difference compared to the respective control (**** p ≤0.0001, *** p ≤0.001, ** p ≤0.01, * p ≤0.05; Sidak’s test), ns = non-significant different.

Article Snippet: In order to investigate the regulation of the proteins involved in angiogenesis and inflammation processes, specific antibody arrays were performed using RayBiotech®® C-Series Human Angiogenesis Array C1000 (code: AAH-ANG-1000, RayBiotech, Peachtree Corners, GA, USA) and RayBiotech®® C-Series Human Inflammation Array C3 (code: AAH-INF-3, RayBiotech, Peachtree Corners, GA, USA).

Techniques: Inhibition, Expressing, Binding Assay, Control

Journal: Antioxidants (Basel, Switzerland)

Article Title: The Carotenoid Diatoxanthin Modulates Inflammatory and Angiogenesis Pathways In Vitro in Prostate Cancer Cells.

doi: 10.3390/antiox12020359

Figure Lengend Snippet: Figure 3. Assessment of vascular mimicry and angiogenesis inhibition on extracellular matrix. (A) Chemopreventive effects of Dt at three concentrations on DU145 cells compared to DU145 cells growth treated with saponin. (B,C) Capillary like morphogenesis of DU145 cells in absence or presence of Dt after 6 h (B) or 24 h (C) of treatment. (D) Capillary like morphogenesis of HUVEC cells in absence or presence of Dt after 24 h of treatment. Asterisks indicate the statistically significant difference compared to the respective control (**** p ≤0.0001, *** p ≤0.001, ** p ≤0.01, * p ≤0.05; Sidak’s test), ns = non-significant different.

Article Snippet: In order to investigate the regulation of the proteins involved in angiogenesis and inflammation processes, specific antibody arrays were performed using RayBiotech®® C-Series Human Angiogenesis Array C1000 (code: AAH-ANG-1000, RayBiotech, Peachtree Corners, GA, USA) and RayBiotech®® C-Series Human Inflammation Array C3 (code: AAH-INF-3, RayBiotech, Peachtree Corners, GA, USA).

Techniques: Inhibition, Control