c 12005 Search Results


94
PromoCell human normal epidermal keratinocytes nhek
Human Normal Epidermal Keratinocytes Nhek, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human normal epidermal keratinocytes nhek/product/PromoCell
Average 94 stars, based on 1 article reviews
human normal epidermal keratinocytes nhek - by Bioz Stars, 2026-05
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90
Creative Biolabs primary normal human epidermal keratinocytes c-12005
Effect of TRPC7 on cell proliferation in lung adenocarcinoma. (A) Immunoblot detection of the protein expression level of TRPC7 in lung cell lines. Normal lung cell lines were BEAS-2B (human bronchial epithelium), BES-1A1.6 (human bronchial epithelium) and IMR-90 (human lung fibroblast). Squamous cell carcinoma cell lines were H125 and H520. Adenocarcinoma cell lines were A549 and H1299. KC were used as TRPC7-positive control cells. (B) Further comparison of endogenous TRPC7 protein expression levels among normal lung, squamous cell carcinoma and adenocarcinoma cell lines using LC/MS. Quantification of the TRPC7 expression analyzed using unpaired Student's t-test. Data were presented as mean ± SD. Effect of (C) TRPC7 knockdown and (D) TRPC7 overexpression on H1299 and BEAS-2B cell proliferation (analyzed using unpaired t-test), respectively. Reverse transcription-quantitative PCR and immunoblotting analysis demonstrated that TRPC7 mRNA and protein expression levels were (E) reduced in H1299 and (F) overexpressed in BEAS-2B cells (analyzed using unpaired t-test). The percentage of (G) TRPC7 knockdown H1299 and (H) TRPC7-overexpressing BEAS-2B cells in the G0/G1, S and G2/M phases (analyzed using unpaired Student's t-test; n=3). Data were presented as mean ± SD for all experiments. *P<0.05, **P<0.01 and ***P<0.001. KC, primary normal human epidermal <t>keratinocytes;</t> LC/MS, liquid chromatography tandem mass spectrometry; OE, overexpression; si, small interfering RNA; TRPC7, transient receptor potential canonical 7.
Primary Normal Human Epidermal Keratinocytes C 12005, supplied by Creative Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary normal human epidermal keratinocytes c-12005/product/Creative Biolabs
Average 90 stars, based on 1 article reviews
primary normal human epidermal keratinocytes c-12005 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

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Effect of TRPC7 on cell proliferation in lung adenocarcinoma. (A) Immunoblot detection of the protein expression level of TRPC7 in lung cell lines. Normal lung cell lines were BEAS-2B (human bronchial epithelium), BES-1A1.6 (human bronchial epithelium) and IMR-90 (human lung fibroblast). Squamous cell carcinoma cell lines were H125 and H520. Adenocarcinoma cell lines were A549 and H1299. KC were used as TRPC7-positive control cells. (B) Further comparison of endogenous TRPC7 protein expression levels among normal lung, squamous cell carcinoma and adenocarcinoma cell lines using LC/MS. Quantification of the TRPC7 expression analyzed using unpaired Student's t-test. Data were presented as mean ± SD. Effect of (C) TRPC7 knockdown and (D) TRPC7 overexpression on H1299 and BEAS-2B cell proliferation (analyzed using unpaired t-test), respectively. Reverse transcription-quantitative PCR and immunoblotting analysis demonstrated that TRPC7 mRNA and protein expression levels were (E) reduced in H1299 and (F) overexpressed in BEAS-2B cells (analyzed using unpaired t-test). The percentage of (G) TRPC7 knockdown H1299 and (H) TRPC7-overexpressing BEAS-2B cells in the G0/G1, S and G2/M phases (analyzed using unpaired Student's t-test; n=3). Data were presented as mean ± SD for all experiments. *P<0.05, **P<0.01 and ***P<0.001. KC, primary normal human epidermal keratinocytes; LC/MS, liquid chromatography tandem mass spectrometry; OE, overexpression; si, small interfering RNA; TRPC7, transient receptor potential canonical 7.

Journal: Oncology Letters

Article Title: TRPC7 facilitates cell growth and migration by regulating intracellular Ca 2+ mobilization in lung adenocarcinoma cells

doi: 10.3892/ol.2023.13678

Figure Lengend Snippet: Effect of TRPC7 on cell proliferation in lung adenocarcinoma. (A) Immunoblot detection of the protein expression level of TRPC7 in lung cell lines. Normal lung cell lines were BEAS-2B (human bronchial epithelium), BES-1A1.6 (human bronchial epithelium) and IMR-90 (human lung fibroblast). Squamous cell carcinoma cell lines were H125 and H520. Adenocarcinoma cell lines were A549 and H1299. KC were used as TRPC7-positive control cells. (B) Further comparison of endogenous TRPC7 protein expression levels among normal lung, squamous cell carcinoma and adenocarcinoma cell lines using LC/MS. Quantification of the TRPC7 expression analyzed using unpaired Student's t-test. Data were presented as mean ± SD. Effect of (C) TRPC7 knockdown and (D) TRPC7 overexpression on H1299 and BEAS-2B cell proliferation (analyzed using unpaired t-test), respectively. Reverse transcription-quantitative PCR and immunoblotting analysis demonstrated that TRPC7 mRNA and protein expression levels were (E) reduced in H1299 and (F) overexpressed in BEAS-2B cells (analyzed using unpaired t-test). The percentage of (G) TRPC7 knockdown H1299 and (H) TRPC7-overexpressing BEAS-2B cells in the G0/G1, S and G2/M phases (analyzed using unpaired Student's t-test; n=3). Data were presented as mean ± SD for all experiments. *P<0.05, **P<0.01 and ***P<0.001. KC, primary normal human epidermal keratinocytes; LC/MS, liquid chromatography tandem mass spectrometry; OE, overexpression; si, small interfering RNA; TRPC7, transient receptor potential canonical 7.

Article Snippet: H125 and the primary normal human epidermal keratinocytes (cat. no. C-12005) were purchased from Creative Biolabs, Inc. and PromoCell GmbH., respectively.

Techniques: Western Blot, Expressing, Positive Control, Comparison, Liquid Chromatography with Mass Spectroscopy, Knockdown, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Liquid Chromatography, Mass Spectrometry, Small Interfering RNA