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Image Search Results
Journal: Oncology Letters
Article Title: TRPC7 facilitates cell growth and migration by regulating intracellular Ca 2+ mobilization in lung adenocarcinoma cells
doi: 10.3892/ol.2023.13678
Figure Lengend Snippet: Effect of TRPC7 on cell proliferation in lung adenocarcinoma. (A) Immunoblot detection of the protein expression level of TRPC7 in lung cell lines. Normal lung cell lines were BEAS-2B (human bronchial epithelium), BES-1A1.6 (human bronchial epithelium) and IMR-90 (human lung fibroblast). Squamous cell carcinoma cell lines were H125 and H520. Adenocarcinoma cell lines were A549 and H1299. KC were used as TRPC7-positive control cells. (B) Further comparison of endogenous TRPC7 protein expression levels among normal lung, squamous cell carcinoma and adenocarcinoma cell lines using LC/MS. Quantification of the TRPC7 expression analyzed using unpaired Student's t-test. Data were presented as mean ± SD. Effect of (C) TRPC7 knockdown and (D) TRPC7 overexpression on H1299 and BEAS-2B cell proliferation (analyzed using unpaired t-test), respectively. Reverse transcription-quantitative PCR and immunoblotting analysis demonstrated that TRPC7 mRNA and protein expression levels were (E) reduced in H1299 and (F) overexpressed in BEAS-2B cells (analyzed using unpaired t-test). The percentage of (G) TRPC7 knockdown H1299 and (H) TRPC7-overexpressing BEAS-2B cells in the G0/G1, S and G2/M phases (analyzed using unpaired Student's t-test; n=3). Data were presented as mean ± SD for all experiments. *P<0.05, **P<0.01 and ***P<0.001. KC, primary normal human epidermal keratinocytes; LC/MS, liquid chromatography tandem mass spectrometry; OE, overexpression; si, small interfering RNA; TRPC7, transient receptor potential canonical 7.
Article Snippet: H125 and the primary
Techniques: Western Blot, Expressing, Positive Control, Comparison, Liquid Chromatography with Mass Spectroscopy, Knockdown, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Liquid Chromatography, Mass Spectrometry, Small Interfering RNA