Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: Gene expression of mNSCs and cytokine profiling of HUVEC. (a) Heatmap of 92 genes, including housekeeping genes, depicts the neurogenesis-associated mRNA expression in mNSC compared to BV2 cells. (b) A scatterplot indicates a greater than 12-fold difference in neurogenesis-related values between mNSCs and BV2 cells, with red spots emphasized. (c) Heatmap of 92 genes, including housekeeping genes, illustrates the mRNA expression associated with ECM and adhesion molecules in mNSCs compared to BV2 cells. (d) A scatterplot displays a more than 10-fold difference in ECM and adhesion molecule values between mNSCs and BV2 cells with red spots emphasized. (e) mRNA expressions of tPA and uPA in mNSCs and BV2 batches. (f) and (g) Cytokine expression profile of HUVEC to investigate the potential effects on ECM secretion.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: Gene Expression, Expressing

Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: Transduction of GFP and mCherry into mNSCs, BV2, and HUVECs. (a) Representative images depict the vector designs used for lentiviral transduction of EGFP and mCherry. (b) Fluorescence microscopy of living cells showing EGFP expression in mNSCs and mCherry expression in BV2 and HUVEC cells, respectively. Scale bars measure 100 μ m. (c) Quantitative analysis of fluorescence intensities in individual cells using flow cytometry. The blue histogram represents the isotype control and the red histogram indicates positive cells. The percentage reflects gated population.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: Transduction, Plasmid Preparation, Fluorescence, Microscopy, Expressing, Flow Cytometry, Control

Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: In vitro assay of mNSCs in co-culture system with BV2 and HUVEC. (a) Representative images show cell morphologies and adhesions on Matrigel prior to co-culture use. Scale bars measure 200 μ m. (b) Integration and migration of mNSCs within a HUVEC co-culture environment, not seen in BV2 cells. Scale bars measure 50 μ m. (c) mNSCs demonstrate high affinity for the vascular-like structure from which HUVEC were removed. Scale bars are 200 and 100 μ m.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: In Vitro, Co-Culture Assay, Migration