Journal: Neuropharmacology

Article Title: Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism.

doi: 10.1016/j.neuropharm.2023.109634

Figure Lengend Snippet: Fig. 2. Acute (1h) and residual (24h) effects of single buspirone or saline (Sa) injection on anxiety-like behavior in the elevated plus maze (A,B,C) and social approach behavior in the modified three chamber test (D,E) in B6 and BTBR mice (male, open, and female, close). A. distance traveled, B. time spent head dipping, and C. percentage of time spent in open arms in the EPM. D. Confronted with a familiar (e.g., same strain) opponents and E. Confronted with an unfamiliar (e.g., different strain) opponents in the three-chamber test. 50% social approach shows a chance level of investigation between empty vs. social bins. * indicates significant drug effects, p < 0.05. n.s., Not significant in drug effects. + indicates sex effects in group.

Article Snippet: The serotonin stimulant, buspirone, a 5-HT1A receptor agonist (Cat#. FP19364, Carbosynth) was dissolved in sterile saline (0.9%, pH7.4) at a concentration of 2 mg/10 mL was administered to each mouse before the test session.

Techniques: Saline, Injection, Modification

Journal: Neuropharmacology

Article Title: Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism.

doi: 10.1016/j.neuropharm.2023.109634

Figure Lengend Snippet: Fig. 3. Neural activity patterns of several brain regions in response to acute buspirone (B) or saline (S) injection in B6 and BTBR mice. Induction of c-Fos as a neural activity marker was measured in (A) the cingulate cortex, (B) subventricular zone (SVZ), (C) bed nucleus of the stria terminalis (BnST), (D) hippocampus including the dentate gyrus (DG), C1, C3, and ventral portion, (E) the paraventricular nucleus of the hypothalamus (PVN), and (F) amygdala of the medial portion (MeA) and basolateral portion (BLA). The densities were calculated as the number of c-Fos positive cells in 100 μm2 region area. * indicates significant drug effects, p < 0.05. and # indicates strain differences, p < 0.05. n.s., Not significant.

Article Snippet: The serotonin stimulant, buspirone, a 5-HT1A receptor agonist (Cat#. FP19364, Carbosynth) was dissolved in sterile saline (0.9%, pH7.4) at a concentration of 2 mg/10 mL was administered to each mouse before the test session.

Techniques: Activity Assay, Saline, Injection, Marker

Journal: Neuropharmacology

Article Title: Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism.

doi: 10.1016/j.neuropharm.2023.109634

Figure Lengend Snippet: Fig. 4. The mRNA expression of neurogenetic factors (Nestin, DCX, BDNF), 5-HT related factors (Tph2, Pet-1, 5HTR1a), and pro-inflammatory factors (IL-1β, and IL- 6), in several brain regions, including the cingulate cortex, subventricular zone (SVZ), hippocampus, and raphe nuclei of B6 and BTBR mice that received either saline (S) or buspirone (B) injection. Expression levels are calculated as fold changes to a B6 saline control. * indicates significant drug effects, p < 0.05. and # indicates strain differences, p < 0.05. n.s., Not significant.

Article Snippet: The serotonin stimulant, buspirone, a 5-HT1A receptor agonist (Cat#. FP19364, Carbosynth) was dissolved in sterile saline (0.9%, pH7.4) at a concentration of 2 mg/10 mL was administered to each mouse before the test session.

Techniques: Expressing, Saline, Injection, Control

Journal: Neuropharmacology

Article Title: Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism.

doi: 10.1016/j.neuropharm.2023.109634

Figure Lengend Snippet: Fig. 5. The mRNA expression of neurogenetic factors (BDNF), 5-HT related factors (5HTR1a), and pro-inflammatory factors (IL-1β, and IL-6) in several brain regions, including the hypothalamus, medial amygdala (MeA), and basolateral amygdala (BLA) of B6 and BTBR mice that received either saline (S) or buspirone (B) injection. Expression levels are calculated as fold changes to a B6 saline control. * indicates significant drug effects, p < 0.05. and # indicates strain differences, p < 0.05. small #, p < 0.10. n.s., Not significant.

Article Snippet: The serotonin stimulant, buspirone, a 5-HT1A receptor agonist (Cat#. FP19364, Carbosynth) was dissolved in sterile saline (0.9%, pH7.4) at a concentration of 2 mg/10 mL was administered to each mouse before the test session.

Techniques: Expressing, Saline, Injection, Control

Journal: Neuropharmacology

Article Title: Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism.

doi: 10.1016/j.neuropharm.2023.109634

Figure Lengend Snippet: Fig. 6. Protein concentration of pro- and mature BDNF in whole brain of B6 and BTBR mice that were received either saline (S) or buspirone (B) injection. Protein levels are pg/mg total protein. n.s., Not significant.

Article Snippet: The serotonin stimulant, buspirone, a 5-HT1A receptor agonist (Cat#. FP19364, Carbosynth) was dissolved in sterile saline (0.9%, pH7.4) at a concentration of 2 mg/10 mL was administered to each mouse before the test session.

Techniques: Protein Concentration, Saline, Injection