bun colorimetric detection kit Search Results


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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
Tunel Based Titertacs Colorimetric Apoptosis Detection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
Glutathione Colorimetric Detection Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
Bun Colorimetric Detection Kit, supplied by Arbor Assays, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Figure 4. CoREST inhibition reduces colony formation and induces <t>apoptosis</t> in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.
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Image Search Results


Figure 4. CoREST inhibition reduces colony formation and induces apoptosis in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.

Journal: Journal of Clinical Investigation

Article Title: The CoREST repressor complex mediates phenotype switching and therapy resistance in melanoma

doi: 10.1172/jci171063

Figure Lengend Snippet: Figure 4. CoREST inhibition reduces colony formation and induces apoptosis in BRAFi-R melanoma cells with or without BRAFi. (A) Colony formation assay quantification in 451Lu-R (left) and 1205Lu-R (right) melanoma cells treated with DMSO, 5 μM PLX4032 alone, 2.5 μM corin alone, or 2.5 μM corin plus 5 μM PLX4032 for 10 days (n = 3). (B) Quantification of TUNEL+ cells in 451Lu-R and 1205Lu-R melanoma cells following 72 hours of treatment with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 (n = 3). (C) Senescence-associated β-galactosidase staining of 451Lu-R melanoma cells treated with DMSO, 5 μM PLX4032 alone, 1 μM corin alone, or 1 μM corin plus 5 μM PLX4032 for 72 hours and quantification (n = 2–3). Scale bar: 50 μm. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test.

Article Snippet: Apoptosis was detected used the TUNEL-based TiterTACS Colorimetric Apoptosis Detection Kit (R&D Systems).

Techniques: Inhibition, Colony Assay, TUNEL Assay, Staining