Journal: The EMBO Journal

Article Title: Genome‐wide cooperation of EMT transcription factor ZEB 1 with YAP and AP ‐1 in breast cancer

doi: 10.15252/embj.2019103209

Figure Lengend Snippet: Immunofluorescence staining of ZEB1, the AP‐1 factors JUN and FOSL1, and the Hippo pathway effector TAZ in MDA‐MB‐231 cells. All four proteins show a clear nuclear localisation. Scale bar = 20 μm. In situ proximity ligation assay (PLA) of ZEB1 with JUN, FOSL1 and TAZ shows close proximity of ZEB1 with JUN and FOSL1 but not TAZ in the nucleus of BT549 and Hs578T breast cancer cells indicated by red fluorescent dots. In the upper panel, representative microscopic images are shown. Scale bar = 20 μm. The lower panel shows a quantification of the PLA shown above. n = 2–3; shown is one representative experiment with means ± s.e.m. of at least 900 cells per condition; *** P ≤ 0.001; 2‐way ANOVA plus Tukey′s post‐test.

Article Snippet: MDA‐MB‐231, MCF7, BT549, Hs578T and MCF10A cells were purchased from the American Type Culture Collection (ATCC).

Techniques: Immunofluorescence, Staining, In Situ, Proximity Ligation Assay

Journal: The EMBO Journal

Article Title: Genome‐wide cooperation of EMT transcription factor ZEB 1 with YAP and AP ‐1 in breast cancer

doi: 10.15252/embj.2019103209

Figure Lengend Snippet: qRT–PCR of ZEB1, FOSL1 and YAP, 11 ZEB1/YAP/JUN‐activated target genes and one ZEB1‐only repressed target gene, CDH1, in MCF10A cells after 7 days of TGFβ treatment compared to control treated cells. Both, control and TGFß‐treated cells were treated with control siRNA for 72 h before harvest. n = 3; shown is mean ± s.e.m. Luciferase reporter assay in BT549 and Hs578T cells comparing control and transient siRNA‐mediated knockdown of ZEB1. Reporter constructs of regulatory regions of genes shown in Fig (B) and (C). n = 3; shown is mean ± s.e.m.; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; unpaired two‐tailed Student's t ‐test.

Article Snippet: MDA‐MB‐231, MCF7, BT549, Hs578T and MCF10A cells were purchased from the American Type Culture Collection (ATCC).

Techniques: Quantitative RT-PCR, Control, Luciferase, Reporter Assay, Knockdown, Construct, Two Tailed Test

Journal: The EMBO Journal

Article Title: Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputs

doi: 10.15252/embj.2020107182

Figure Lengend Snippet:

Article Snippet: BT549 , LGC ATCC® , HTB‐122.

Techniques: Virus, Recombinant, Extraction, Multiplex sample analysis, Transfection, Molecular Weight, Marker, Reverse Transcription, Protease Inhibitor, Membrane, Cell Culture, Clinical Proteomics, Spectroscopy, Imaging, cDNA Synthesis, Plasmid Preparation, Negative Control, Mutagenesis, Clone Assay, Generated, Software, Microscopy

Journal: iScience

Article Title: Conserved role of FOXC1 in TNBC is parallel to FOXA1 in ER+ breast cancer

doi: 10.1016/j.isci.2024.110500

Figure Lengend Snippet: Identification of FOXC1 cofactors (A) Protein co-interactors of FOXC1 identified in Hs578t and BT-549 via RIME in two biological replicates, after subtracting those identified in negative IgG control. (B) Western blot depicting co-immunoprecipitation of FOXC1 using antibody against NR2F2 with input and IgG negative controls.

Article Snippet: Bt549 , AddexBio , C0006017, RRID:CVCL_1092.

Techniques: Control, Western Blot, Immunoprecipitation

Journal: iScience

Article Title: Conserved role of FOXC1 in TNBC is parallel to FOXA1 in ER+ breast cancer

doi: 10.1016/j.isci.2024.110500

Figure Lengend Snippet:

Article Snippet: Bt549 , AddexBio , C0006017, RRID:CVCL_1092.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Sequencing, Generated, Gene Expression, Mass Spectrometry, Knock-Out, Software, Blocking Assay