Journal: Clinical science (London, England : 1979)

Article Title: Sodium bicarbonate loading limits tubular cast formation independent of glomerular injury and proteinuria in Dahl salt-sensitive rats

doi: 10.1042/CS20171630

Figure Lengend Snippet: Reagents/antibodies

Article Snippet: Histograms show the expression of IL-10 by M2 macrophages to demonstrate and confirm the activated M2 macrophages ( E , the graphs with RED line represent the IL-10 expression while the filled GREY curves show the isotype control) table ft1 table-wrap mode="anchored" t5 caption a7 Antibody name Company Catalog # Lot # Tube amount Species CD11b/c-PE, OX-42 BD Pharmingen 554862 1.0 ml anti-rat CD11b/c-PE, OX-42 eBioscience 12-0110-80 {"type":"entrez-nucleotide","attrs":{"text":"E15919","term_id":"5710602","term_text":"E15919"}} E15919 –106 125 μl/500 μ1 α -rat CD206 PerCP/Cy 5.5 Biolegend 321122 B175845 0.5 ml anti-human F4/80-A488, EMRI Bioss bs-7058R-A488 AE084243 100 μ1 rabbit IL-10-Alexa647, CC320 Novus NB100–63026AF6477 1007–081415 250 μ1 mouse ab TNF α -biotin Bioss bs-2081R-biotin AE081731 100 μ1 rabbit Streptavidin-APC eBioscience 17-4317-82 {"type":"entrez-nucleotide","attrs":{"text":"E07261","term_id":"2175402","term_text":"E07261"}} E07261 –1634 0.5 ml Collagenase IV Sigma (St. Louis, MO) C5138–500mg 500 mg ACK Lysing Buffer Quality Biological 50983220 4 × 100 ml Open in a separate window Reagents/antibodies Samples were double-stained with control IgG and cell markers and were used to assess any spillover signal of fluorochromes; proper compensation was set to ensure that the median fluorescence intensities of negative and positive cells were identical and were both gated populations.

Techniques: