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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
doi: 10.3390/ani10081361
Figure Lengend Snippet: ITGB1 inhibition affects downstream gene expression. ( A ) Western blotting images of proteins related to the ITGB1-mediated signaling pathway at 48 h after ITGB1 inhibition. ( B – G ) Quantification of the ITGB1, p-FAK, p-paxillin, vinculin, Cdc42, and Arp2/3 Western blotting results presented in A. ** p < 0.01.
Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R),
Techniques: Inhibition, Expressing, Western Blot
Journal: Animals : an Open Access Journal from MDPI
Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
doi: 10.3390/ani10081361
Figure Lengend Snippet: SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.
Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R),
Techniques: Western Blot, Activation Assay, Inhibition
Journal: Animals : an Open Access Journal from MDPI
Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
doi: 10.3390/ani10081361
Figure Lengend Snippet: SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R),
Techniques: Western Blot, Expressing, Activation Assay, Inhibition, Negative Control
Journal: Animals : an Open Access Journal from MDPI
Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
doi: 10.3390/ani10081361
Figure Lengend Snippet: Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.
Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R),
Techniques: Migration, Expressing