Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: Technical roadmap for the diagnosis of nmCRPC by USMI based on MBs NRP2

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques:

Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: a Study design for immunofluorescence localization staining. b Confocal Laser Scanning Microscope(CLSM) images and colocalization analysis for immunofluorescence localization staining of CD31 and NRP2 in human prostate cancer tissues. c Fluorescence intensity quantification of NRP2. n = 5 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques: Immunofluorescence, Staining, Laser-Scanning Microscopy, Fluorescence

Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: a Schematic diagram of the synthesis of MBs NRP2 b Size of MBs Control and MBs NRP2 . c Zeta Potential of MBs Control and MBs NRP2 . d FTIR spectra of MBs Control and MBs NRP2 . e CLSM images of MBs Control and MBs NRP2 . f Binding rate of MBs NRP2

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques: Control, Zeta Potential Analyzer, Binding Assay

Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: a CLSM images and colocalization for immunofluorescence localization staining of CD31 and NRP2. b Schematic diagram of the transwell co—culture. c Fluorescence intensity quantification of NRP2. d NRP2 expression was depleted in HMEC- 1 cells using different prostate cancer cell lines as stimuli. β-actin was used as a loading control. e Flow cytometric analyses of HMEC- 1 cells exposed to different prostate cancer cell lines. f Prostate cancer cells exhibited the ability to promote tube formation in endothelial cells. g Quantifi1cation of tube formation exposed to different prostate cancer cells. n = 5 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques: Immunofluorescence, Staining, Co-Culture Assay, Fluorescence, Expressing, Control

Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: a Study design for the Flow Chamber experiment. b MBs NRP2 binding on control HMEC- 1 cells and HMEC- 1 cells exposed to prostate cancer cells (VCaP cells,22RV1 cells, and DU145 cells) under shear stress (0.6 mL/min). c Quantification of MBs NRP2 binding to HMEC- 1 cells in a single field of view. n = 5 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques: Binding Assay, Control, Shear

Journal: BMC Cancer

Article Title: MBs NRP2 -based ultrasound molecular imaging for early diagnosis of castration-resistant prostate cancer

doi: 10.1186/s12885-025-14143-7

Figure Lengend Snippet: a Study design for contrast ultrasound imaging of a mouse. b Study design for ultrasound-induced microbubble destruction and replenishment. c B-mode images from tumor-bearing mice and non-tumor-bearing mice; USMI images obtained with all MBs NRP2 and freely circulated MBs NRP2 before and post the destructive pulse and in tumor-bearing mice and non-tumor-bearing mice. The image panel presents ultrasound B-mode images in grey (upper row) and the respective contrast-enhanced ultrasound image in brown (lower row) including the color-coded USMI dTE signal distribution ((pre-burst)—(post-burst)) in the region of interest (green contour), a local area of the tumor(blue contour), and reference region for USMI(yellow contour). d Study design for attached MBs NRP2 . e Quantification of attached MBs NRP2 . f CLSM images and colocalization for immunofluorescence localization staining of CD31 and NRP2 in tumor-bearing mice and non-tumor-bearing mice. g Fluorescence intensity quantification of NRP2 in tumor-bearing mice and non-tumor-bearing mice. h Fluorescence intensity quantification of NRP2 expression in tumor-bearing mice and non-tumor-bearing mice. i IHC percentage quantification of NRP2-positive cells in tumor-bearing mice and non-tumor-bearing mice. n = 6 samples per group; *, p < 0.05; **, p < 0.01; ***, p < 0.005; error bars present SD

Article Snippet: MBs NRP2 were prepared using streptavidin–biotin binding chemistry to target rabbit anti-NRP2 biotin-conjugated antibodies (bs- 10241R-Bio, Bioss).

Techniques: Imaging, Immunofluorescence, Staining, Fluorescence, Expressing