bs-10376R-Biotin Search Results


anti cd86  (Bioss)
90
Bioss anti cd86
Anti Cd86, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd86/product/Bioss
Average 90 stars, based on 1 article reviews
anti cd86 - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti mouse α actinin biotin  (Bioss)
91
Bioss rabbit anti mouse α actinin biotin
Cardiac CD8+ T cells require perforin to promote fibrosis. (A) Representative photomicrographs indicating perforin expression by CD8+ T cells in fibrotic ventricle of TAC mice. (B) Bar graphs indicating that 4-weeks after bone marrow transplantation major lymphocyte populations have recovered to levels seen in non-irradiated C57BL/6 mice. (C) Representative FACS plots indicating that spleen CD8+ T cells in CD8T-Pfp -/- mice are deficient in perforin 4 weeks after BMT compared to CD8T-Pfpf +/+ mice. (D) Photomicrographs (PSR) together with mean data indicating that perforin deletion in CD8+ T cells attenuates fibrosis in LV of TAC mice compared to controls. (E) Photomicrographs indicating that <t>TUNEL+Actinin+</t> cardiomyocytes are greatly reduced in mice with perforin deficient CD8+ T cells. (F) LV CD68+ macrophages and (G) TGF-β1 expressing cells are greatly reduced with perforin deletion from CD8+ T cells. Results are means ± SEM with small circles representing data from individual mice. n=5-9/group. PSR; picrosirius, -ve control; no primary antibody control, sham; sham-operated, scale bar represents 50µm. *P< 0.05 using two-tailed Student’s t-test comparing to CD8T-Pfp +/+ .
Rabbit Anti Mouse α Actinin Biotin, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti mouse α actinin biotin/product/Bioss
Average 91 stars, based on 1 article reviews
rabbit anti mouse α actinin biotin - by Bioz Stars, 2026-02
91/100 stars
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orexin a polyclonal antibody, biotin conjugated  (Bioss)
N/A
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin
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Image Search Results


Cardiac CD8+ T cells require perforin to promote fibrosis. (A) Representative photomicrographs indicating perforin expression by CD8+ T cells in fibrotic ventricle of TAC mice. (B) Bar graphs indicating that 4-weeks after bone marrow transplantation major lymphocyte populations have recovered to levels seen in non-irradiated C57BL/6 mice. (C) Representative FACS plots indicating that spleen CD8+ T cells in CD8T-Pfp -/- mice are deficient in perforin 4 weeks after BMT compared to CD8T-Pfpf +/+ mice. (D) Photomicrographs (PSR) together with mean data indicating that perforin deletion in CD8+ T cells attenuates fibrosis in LV of TAC mice compared to controls. (E) Photomicrographs indicating that TUNEL+Actinin+ cardiomyocytes are greatly reduced in mice with perforin deficient CD8+ T cells. (F) LV CD68+ macrophages and (G) TGF-β1 expressing cells are greatly reduced with perforin deletion from CD8+ T cells. Results are means ± SEM with small circles representing data from individual mice. n=5-9/group. PSR; picrosirius, -ve control; no primary antibody control, sham; sham-operated, scale bar represents 50µm. *P< 0.05 using two-tailed Student’s t-test comparing to CD8T-Pfp +/+ .

Journal: Frontiers in Immunology

Article Title: Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts

doi: 10.3389/fimmu.2022.1040233

Figure Lengend Snippet: Cardiac CD8+ T cells require perforin to promote fibrosis. (A) Representative photomicrographs indicating perforin expression by CD8+ T cells in fibrotic ventricle of TAC mice. (B) Bar graphs indicating that 4-weeks after bone marrow transplantation major lymphocyte populations have recovered to levels seen in non-irradiated C57BL/6 mice. (C) Representative FACS plots indicating that spleen CD8+ T cells in CD8T-Pfp -/- mice are deficient in perforin 4 weeks after BMT compared to CD8T-Pfpf +/+ mice. (D) Photomicrographs (PSR) together with mean data indicating that perforin deletion in CD8+ T cells attenuates fibrosis in LV of TAC mice compared to controls. (E) Photomicrographs indicating that TUNEL+Actinin+ cardiomyocytes are greatly reduced in mice with perforin deficient CD8+ T cells. (F) LV CD68+ macrophages and (G) TGF-β1 expressing cells are greatly reduced with perforin deletion from CD8+ T cells. Results are means ± SEM with small circles representing data from individual mice. n=5-9/group. PSR; picrosirius, -ve control; no primary antibody control, sham; sham-operated, scale bar represents 50µm. *P< 0.05 using two-tailed Student’s t-test comparing to CD8T-Pfp +/+ .

Article Snippet: After washings, sections were incubated in 10% normal serum/PBS before being incubated with primary antibodies in serum: rabbit anti-mouse CD8 (Sino Biological: cat#50389-R20), mouse anti-mouse NK1.1 (Biolegend: cat#108701), Armenian Hamster- anti-mouse TCR γ/δ (Biolegend: cat#108101), rat anti-mouse Perforin (LS Biosciences: cat#LS-C18632), biotin rat anti-mouse NKG2D (R&D Systems; cat#BAM1547), rabbit anti-mouse TGFβ1 (Novus Biological: cat#NB100-91995), rat anti-mouse CD68 (BioRad: cat#MCA1957), rabbit anti-mouse troponin (Abcam: cat#ab125266), goat anti-mouse RAE-1 (R&D Systems: cat#AF1136), rabbit anti-mouse α-Actinin (Acris Antibodies: cat#BP210), rabbit anti-mouse phosphor-STING (Invitrogen: cat# PA5-105674), goat anti-mouse CX3CR1 (R & D Systems: cat#AF5825), mouse anti-mouse phospho p38MAPK (Biolegend: cat#690201), rabbit anti-mouse phospho-IRF3 (Cell Signaling: cat#29047), rabbit anti-mouse phospho-TBK1 (Imgenex: cat# IMX-5194), mouse anti-mouse NF-kB (Santa Cruz: cat# sc-109), rabbit anti-mouse MCP-1 (Abcam: cat #ab7202), rabbit anti-mouse α-Actinin Biotin (Bioss antibodies: cat# BS-10367R-Biotin) or corresponding isotype matched control IgG antibodies.

Techniques: Expressing, Transplantation Assay, Irradiation, TUNEL Assay, Two Tailed Test

Cardiac CD8+ T cells require NKG2D receptors to promote fibrosis. (A) Photomicrographs indicating that CD8+ cells accumulated in LV of TAC mice express NKG2D receptor. (B) CD8+ T cells of TAC mice that underwent bone marrow transplantation with mixed bone marrow deficient in NKG2D (CD8T-NKG2D -/- ) do not express NKG2D compared to controls (CD8T-NKG2D +/+ ). (C) LV fibrosis is attenuated in TAC mice with NKG2D-deficient CD8+ T cells. (D) TUNEL+ Actinin+ cardiomyocytes are greatly reduced in mice with NKG2D-deficient CD8+ T cells. (E, F) LV CD68+ macrophages and TGF-β1 expressing cells in TAC mice with NKG2D-deficient CD8+ T cells are markedly reduced. Results are means ± SEM with small circles representing data from individual mice. n=7-9/group. PSR; picrosirius, -ve control; no primary antibody control, scale bar represents 50µm. *P< 0.05 using two tailed Student’s t-test comparing to CD8T-NKG2D +/+ .

Journal: Frontiers in Immunology

Article Title: Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts

doi: 10.3389/fimmu.2022.1040233

Figure Lengend Snippet: Cardiac CD8+ T cells require NKG2D receptors to promote fibrosis. (A) Photomicrographs indicating that CD8+ cells accumulated in LV of TAC mice express NKG2D receptor. (B) CD8+ T cells of TAC mice that underwent bone marrow transplantation with mixed bone marrow deficient in NKG2D (CD8T-NKG2D -/- ) do not express NKG2D compared to controls (CD8T-NKG2D +/+ ). (C) LV fibrosis is attenuated in TAC mice with NKG2D-deficient CD8+ T cells. (D) TUNEL+ Actinin+ cardiomyocytes are greatly reduced in mice with NKG2D-deficient CD8+ T cells. (E, F) LV CD68+ macrophages and TGF-β1 expressing cells in TAC mice with NKG2D-deficient CD8+ T cells are markedly reduced. Results are means ± SEM with small circles representing data from individual mice. n=7-9/group. PSR; picrosirius, -ve control; no primary antibody control, scale bar represents 50µm. *P< 0.05 using two tailed Student’s t-test comparing to CD8T-NKG2D +/+ .

Article Snippet: After washings, sections were incubated in 10% normal serum/PBS before being incubated with primary antibodies in serum: rabbit anti-mouse CD8 (Sino Biological: cat#50389-R20), mouse anti-mouse NK1.1 (Biolegend: cat#108701), Armenian Hamster- anti-mouse TCR γ/δ (Biolegend: cat#108101), rat anti-mouse Perforin (LS Biosciences: cat#LS-C18632), biotin rat anti-mouse NKG2D (R&D Systems; cat#BAM1547), rabbit anti-mouse TGFβ1 (Novus Biological: cat#NB100-91995), rat anti-mouse CD68 (BioRad: cat#MCA1957), rabbit anti-mouse troponin (Abcam: cat#ab125266), goat anti-mouse RAE-1 (R&D Systems: cat#AF1136), rabbit anti-mouse α-Actinin (Acris Antibodies: cat#BP210), rabbit anti-mouse phosphor-STING (Invitrogen: cat# PA5-105674), goat anti-mouse CX3CR1 (R & D Systems: cat#AF5825), mouse anti-mouse phospho p38MAPK (Biolegend: cat#690201), rabbit anti-mouse phospho-IRF3 (Cell Signaling: cat#29047), rabbit anti-mouse phospho-TBK1 (Imgenex: cat# IMX-5194), mouse anti-mouse NF-kB (Santa Cruz: cat# sc-109), rabbit anti-mouse MCP-1 (Abcam: cat #ab7202), rabbit anti-mouse α-Actinin Biotin (Bioss antibodies: cat# BS-10367R-Biotin) or corresponding isotype matched control IgG antibodies.

Techniques: Transplantation Assay, TUNEL Assay, Expressing, Two Tailed Test