Journal: BioMed Research International

Article Title: A Two-Step Lyssavirus Real-Time Polymerase Chain Reaction Using Degenerate Primers with Superior Sensitivity to the Fluorescent Antigen Test

doi: 10.1155/2014/256175

Figure Lengend Snippet: Rabies virus-positive samples used to assess the diagnostic sensitivity of the lyssavirus qRT-PCR.

Article Snippet: European bat lyssavirus-1 EBLV-1 , ANSES, France 1 , EBLV-1a, France , Mouse brain homogenate , 3.

Techniques: Virus, Diagnostic Assay, Infection, Cell Culture, Isolation

Journal: Molecular Therapy

Article Title: ZFN-Mediated In Vivo Genome Editing Corrects Murine Hurler Syndrome

doi: 10.1016/j.ymthe.2018.10.018

Figure Lengend Snippet: Decreased GAG Storage in ZFN+Donor-Treated MPS I Mice GAG levels present in the indicated tissues at 1 (A) or 4 (B) months post-treatment. Data are shown as mean ± SD. *p < 0.05, when compared to the gender-matched untreated MPS I group. n = 3 per group at 1 month and n = 4–5 per group at 4 months. (C) Levels of dermatan and heparan sulfate in mouse brain homogenates, 4 months post-treatment. Lines indicate mean of each group. All wild-type mice had levels of dermatan sulfate below the lower limit of quantification for this assay (<0.005 μg DS/mg protein lysate). *p < 0.05 for treated versus untreated male MPS I mice. For female MPS I mice, p = 0.0635 when comparing treated and untreated groups. (D) Images taken at 40× magnification from H&E-stained slides indicating the presence or absence of disease-related, highly vacuolated tissue-resident macrophages. Location of tissue resident macrophages indicated by black arrows.

Article Snippet: Mass spectrometry of brain tissue homogenates for levels of dermatan and heparan sulfate was performed at Pacific BioLabs (Hercules, CA).

Techniques: Staining