brain ceramide Search Results


ceramide  (Avanti Polar)
98
Avanti Polar ceramide
Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucosyl (β) ceramide d18  (Croda International Plc)
98
Croda International Plc glucosyl (β) ceramide d18
Glucosyl (β) Ceramide D18, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porcine polar lipid extract  (Croda International Plc)
95
Croda International Plc porcine polar lipid extract
Porcine Polar Lipid Extract, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sphingomyelin and ceramide from bovine brain  (Matreya LLC)
90
Matreya LLC sphingomyelin and ceramide from bovine brain
Sphingomyelin And Ceramide From Bovine Brain, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gm2 (galnacβ1,4(neu5acα2,3)galβ1,4glcβ1,1’-ceramide) bovine brain  (FUJIFILM)
90
FUJIFILM gm2 (galnacβ1,4(neu5acα2,3)galβ1,4glcβ1,1’-ceramide) bovine brain
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Gm2 (Galnacβ1,4(Neu5acα2,3)Galβ1,4glcβ1,1’ Ceramide) Bovine Brain, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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ceramide trihexoside from porcine brain  (Matreya LLC)
90
Matreya LLC ceramide trihexoside from porcine brain
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Ceramide Trihexoside From Porcine Brain, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ceramide from bovine brain  (Matreya LLC)
90
Matreya LLC ceramide from bovine brain
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Ceramide From Bovine Brain, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ceramide from bovine brain - by Bioz Stars, 2026-04
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bovine brain ceramide type iv  (Chemie GmbH)
90
Chemie GmbH bovine brain ceramide type iv
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Bovine Brain Ceramide Type Iv, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bovine brain ceramide type iv - by Bioz Stars, 2026-04
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sheep brain ceramide  (Fluka Chemical)
90
Fluka Chemical sheep brain ceramide
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Sheep Brain Ceramide, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bovine brain ceramides  (Matreya LLC)
90
Matreya LLC bovine brain ceramides
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Bovine Brain Ceramides, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain ceramide  (Matreya LLC)
90
Matreya LLC brain ceramide
Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; <t>GM2,</t> ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes
Brain Ceramide, supplied by Matreya LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ceramide (brain, porcine)  (Aladdin Scientific Corporation)
N/A
Ceramides comprise sphingoid base attached to fatty acyl chain by an amide bond. They vary in length, saturation and hydroxylation of the fatty acid chain and sphingoid base. Ceramides are synthesized by sphingomyelinase in salvage
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Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; GM2, ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Cell density-dependent membrane distribution of ganglioside GM3 in melanoma cells

doi: 10.1007/s00018-023-04813-9

Figure Lengend Snippet: Binding of anti-GM3 antibody (GMR6) to GM3/partner lipid is dependent on incubation temperatures and phase transition temperatures of partner lipids. A Binding of 0.37 µg/mL of anti-GM3 antibody to indicated lipids (0.5 nmol/well) was measured by ELISA. GM1, ganglioside GM1; GM2, ganglioside GM2; GM3, ganglioside GM3; GlcCer, glucosylceramide; LacCer, lactosylceramide; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; pSM, palmitoyl sphingomyelin;Cer, ceramide; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOPS, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine; DOPG, 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); TOCL, 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol; CerPE, N-acyl-sphingosylphosphorylethanolamine. B Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/DOPC (1:9), GM3/POPC (1:9), GM3/DMPC (1:9) or GM3/DPPC (1:9) (0.5 nmol GM3/well) at indicated temperature measured by ELISA. (C) Binding of 0.37 µg/mL of anti-GM3 antibody to GM3/pSM (1:9), GM3/GlcCer (1:9), GM3/GM1 (1:9) or GM3 alone (0.5 nmol GM3/well) at indicated temperatures measured by ELISA. Data in A–C are means ± SD of three experiments. D Binding of anti-GM3 antibody (10 ng/mL) to GM3/DOPC (1:5) (orange) or GM3/DPPC (1:5) (green) liposomes measured by surface plasmon resonance (SPR). SPR was performed as described in Methods. E Binding of anti-GM3 antibody to DPPC/GM3 (5:2) (orange) or DPPC/GM3/Chol (5:2:5) (green) liposomes. F Binding of different concentration of anti-GM3 antibody to GM3/DPPC (1:9) liposomes

Article Snippet: GM2 (GalNAcβ1,4(Neu5Acα2,3)Galβ1,4Glcβ1,1’-ceramide) from bovine brain was from Wako Pure Chemical Industries (Osaka, Japan).

Techniques: Binding Assay, Incubation, Sublimation, Enzyme-linked Immunosorbent Assay, Liposomes, SPR Assay, Concentration Assay