Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: The criteria of Allred scoring system used for evaluating SRPX2 expression in malignant prostate cancer in our study

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Expressing, Staining

Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: RT-PCR and Western blotting of SRPX2 expression in PCa cell lines. Notes: A and B show a representative SRPX2 RT-PCR result and its corresponding histograms, respectively. C and D show a representative Western blotting of SRPX2 and its corresponding histograms, respectively. ** P <0.01, *** P <0.001. Abbreviations: SRPX2, sushi repeat-containing protein X-linked 2; PCa, prostrate cancer.

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing

Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: Immunohistochemical staining of SRPX2 in PCa samples. Notes: The typical diffuse SRPX2 cytoplasmic staining can be found in PCa cells. A and B show strong SRPX2 positivity; C and D show moderate SRPX2 positivity; E and F show SRPX2 negativity (magnification in the left panel: 200×, magnification in the right panel: 400×). Abbreviations: SRPX2, sushi repeat-containing protein X-linked 2; PCa, prostrate cancer.

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Immunohistochemical staining, Staining

Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: The associations of SRPX2 expression levels and the clinicopathological characteristics in prostate cancer

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: Kaplan–Meier survival plot. Notes: High levels of SRPX2 protein expression is significantly associated with a shorter overall survival in PCa ( P <0.001). Abbreviations: SRPX2, sushi repeat-containing protein X-linked 2; PCa, prostrate cancer.

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer

doi: 10.2147/OTT.S158820

Figure Lengend Snippet: Univariate and multivariate analysis for overall survival using Cox relative risk

Article Snippet: After blocking with 5% BSA blocking buffer for 1 hour at room temperature, the membranes were incubated at 4°C overnight with mouse antihuman SRPX2 monoclonal (66266-1-Ig; Proteintech, Wuhan, China).

Techniques: Expressing