Journal: BMC cell biology

Article Title: Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.

doi: 10.1186/1471-2121-11-58

Figure Lengend Snippet: Figure 1 Isolation and validation of PAs interacting with MT1-MMP ICD. (A) Schematic representation of the TrxA scaffold and the PAs swiggle, 76, and s14. The sequences of the peptide inserted in TrxA (swiggle, s14) or fused to the N-terminal region of TrxA (76) are detailed. The point mutation in the sequence of s14 is depicted in bold and the amino acids in lower cases in PA 76 represent linkers between the multiple peptides. * denotes the stop codon. (B) Swiggle interacts with the MT1-MMP ICD in a yeast-two hybrid interaction assay. EGY48 cells expressing LexA-DBD, LexA-MT1, LexA-MT2, LexA-MT3, LexA-MT5 or LexA-Cdk4 were mated with EGY42 cells expressing AD-TrxA, AD-swiggle, AD-s14, AD-76 or AD-CyclinD1 and plated onto selective media.

Article Snippet: Oligonucleotides coding for a PGGG linker followed by MT1-MMP, MT2-MMP, MT3-MMP, or MT5-MMP intracellular domain (ICD) were annealed and cloned downstream of the LexA DNA binding domain (DBD) in pEG202 (Origene, Rockville, USA) to generate LexAMT1, -MT2, -MT3 and -MT5.

Techniques: Isolation, Biomarker Discovery, Mutagenesis, Sequencing, Expressing

Journal: BMC cell biology

Article Title: Peptide aptamers as new tools to modulate clathrin-mediated internalisation--inhibition of MT1-MMP internalisation.

doi: 10.1186/1471-2121-11-58

Figure Lengend Snippet: Figure 8 Swiggle interacts with the LLY573 motif of the MT1-MMP ICD. (A) EGY48 cells expressing LexA-DBD, LexA-MT1, the LexA-MT1 LLY/ A mutant or LexA-Cdk4 were mated with EGY42 cells expressing AD-swiggle, AD-s14 or AD-CyclinD1 and plated onto selective media. (B) Cell lysates prepared from MCF7 cells expressing MT1-MMP alone (lanes 1 and 2) or together with GFP-s14 (lane 3) or GFP-swiggle (lane 4) were subjected to immunoprecipitation in the presence (lanes 2, 3 and 4) or the absence (lane 1) of an anti-MT1-MMP pAb The presence of μ2 and MT1-MMP in the immunocomplexes was monitored by Western blotting. The expression level of GFP-s14, GFP-swiggle or μ2 in the input lysates was analysed by Western blot.

Article Snippet: Oligonucleotides coding for a PGGG linker followed by MT1-MMP, MT2-MMP, MT3-MMP, or MT5-MMP intracellular domain (ICD) were annealed and cloned downstream of the LexA DNA binding domain (DBD) in pEG202 (Origene, Rockville, USA) to generate LexAMT1, -MT2, -MT3 and -MT5.

Techniques: Expressing, Mutagenesis, Immunoprecipitation, Western Blot

Journal: BMC Oral Health

Article Title: Synergistic proanthocyanidin-copper oxygen-generating microneedle enhances anti-inflammatory activity in dental pulp stem cells and macrophage efferocytosis

doi: 10.1186/s12903-026-07870-1

Figure Lengend Snippet: A : Fluorescence staining of ROS levels in macrophages cultured in the leachate of each hydrogel group; ( B ): Semi-quantitative analysis of ROS fluorescence staining; ( C , D , E) : Expression levels of IL-6, IL-1β, and TNF-α in macrophages cultured in the leachate of each hydrogel group; ( F) : Statistical analysis of macrophage efferocytosis index by immunofluorescence staining; G : Statistical analysis of macrophage efferocytosis index by flow cytometry; ( H ): Immunofluorescence staining to detect macrophage efferocytosis levels (blue: nuclei of macrophages; green: cell membrane of macrophages; red: apoptotic hDPSCs); ( I ): Flow cytometry detection of macrophage efferocytosis levels (FITC-A: F4/80-labeled macrophages; PE-A: CellTracker-labeled apoptotic cells)

Article Snippet: Macrophages (RAW264.7) were incubated for 24 h, and 1 mL of DMEM containing 200 ng/mL LPS was added to induce inflammation for 24 h, then 5 × 10 4 stained apoptotic cells and samples of different groups were added and co-cultured for 24 h. Then, it was fixed with 4% paraformaldehyde for 20 min, washed with PBS, blocked with goat serum for 20 min at room temperature, and incubated with F4/80 antibody (Bosterbio, China) overnight at 4°C.

Techniques: Fluorescence, Staining, Cell Culture, Expressing, Immunofluorescence, Flow Cytometry, Membrane, Labeling

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Zengmian Yiliu formula suppresses cell cycle in immune-rich ovarian cancer patient-derived organoids.

doi: 10.1016/j.phymed.2025.156721

Figure Lengend Snippet: Fig. 2. Ovarian cancer organoid characterization. (A) H&E staining of organoids from six patients (OV075, OV076, OV077, OV078, OV080, and OV082), showcasing cellular arrangement and tissue structure. (B) PAX8 staining confirms the organoids’ ovarian epithelial origin. (C) Ki67 staining assesses the organoids’ proliferative activity. Scale Bar: 20 µm.

Article Snippet: For IHC, after deparaffinization and antigen retrieval, the sections were incubated with primary antibodies against Ki67 (MA5-14520, ThermoFisher) and PAX8 (10336-1-AP, Proteintech) at a dilution of 1:1000 overnight at 4◦C.

Techniques: Staining, Activity Assay