Journal: Journal of experimental & clinical cancer research : CR

Article Title: The extracellular matrix protein type I collagen and fibronectin are regulated by β-arrestin-1/endothelin axis in human ovarian fibroblasts.

doi: 10.1186/s13046-025-03327-5

Figure Lengend Snippet: Fig. 3 ET-1 induces Col1 and FN expression through ETAR and ETBR. Representative WB analysis of Col1 and FN protein expression in HOFs stimulated with ET-1 and/or Ambrisentan (AMB) (1 μm) and/or BQ788 (1 μm) and/or bosentan (BOS) (1 μm) for 24 h and 12 h, respectively. GAPDH was used as a loading control. Histograms, mean ± SD. One-way ANOVA (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001

Article Snippet: Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Cat#A11001; Thermo Fisher); The chemical reagents used were as follows: Alexa Fluor 594 phalloidin (Cat# A12381; Thermo Fisher); 4’,6’-diamidino-2-phenykindole (DAPI) (Cat# 1331762; Bio-Rad Laboratories); Vectashield (Cat# H-1000; Vector Laboratories); ET-1 (100 nmol/L) (Cat# E7764-1MG; Sigma-Aldrich), BQ788 (Cat# HY-15894 A, Med Chem Express), Ambrisentan (AMB) (Cat# SML2104; Sigma-Aldrich) also called (+) - (2 S) − 2-[(4,6dimethylpyrimidin-2-yl) oxy]-3-methoxy3,3-diphenylpropanoic acid, Bosentan (BOS) (Cat# HY-A0013A, Med Chem Express).

Techniques: Expressing, Control

Journal: Circulation Research

Article Title: Bosentan Enhances Viral Load via Endothelin-1 Receptor Type-A–Mediated p38 Mitogen-Activated Protein Kinase Activation While Improving Cardiac Function During Coxsackievirus-Induced Myocarditis

doi: 10.1161/circresaha.108.191171

Figure Lengend Snippet: Figure 2. Bosentan alleviates the ET1-mediated loss of cardiac func- tion during CVB3 myocarditis. Mouse cardiac function was examined before infection and 5 days pi by echocardiography as described in Materials and Methods. EF (A) and FS (B) after bosentan treatment are shown as meansSD. *P0.05 as compared to vehicle-treated group. C, Survival curve of vehicle vs bosentan treated mice during CVB3-induced myocarditis.

Article Snippet: Five-week-old A/J mice were infected intraperitoneally with 1.0 105 plaque forming units of CVB3-Gauntt and treated with bosentan and SB203580 HCl (Tocris Pharmaceuticals), starting the following day.

Techniques: Infection

Journal: Circulation Research

Article Title: Bosentan Enhances Viral Load via Endothelin-1 Receptor Type-A–Mediated p38 Mitogen-Activated Protein Kinase Activation While Improving Cardiac Function During Coxsackievirus-Induced Myocarditis

doi: 10.1161/circresaha.108.191171

Figure Lengend Snippet: Figure 3. Myocarditis is exacerbated by bosentan treatment as indicated by increased viral load and global histopathologic scores. A, Mouse heart was harvested 5 days pi, and CVB3 sense and antisense genome were detected by in situ hybridiza- tion using antisense and sense RNA probes, respectively. Bosentan treatment significantly enhances cardiac viral load, presented as percentage of infected area (meansSD). *P0.05 as compared to vehicle-treated group. B, Histopathologic scores (meansSD) by H&E staining were significantly increased in bosentan-treated mice. ‡P0.001 as compared to vehicle-treated group.

Article Snippet: Five-week-old A/J mice were infected intraperitoneally with 1.0 105 plaque forming units of CVB3-Gauntt and treated with bosentan and SB203580 HCl (Tocris Pharmaceuticals), starting the following day.

Techniques: In Situ, Infection, Staining

Journal: Circulation Research

Article Title: Bosentan Enhances Viral Load via Endothelin-1 Receptor Type-A–Mediated p38 Mitogen-Activated Protein Kinase Activation While Improving Cardiac Function During Coxsackievirus-Induced Myocarditis

doi: 10.1161/circresaha.108.191171

Figure Lengend Snippet: Figure 4. Bosentan enhances and prolongs p38 MAPK phosphoryla- tion via ETRA in HL1 cardiomyocytes, leading to enhanced virus prog- eny release. HL1 cells were treated and infected as described in Materials and Methods and as indicated. A, HL1 cells were treated with BQ123 and BQ788 or both simultaneously and subject to ET1 and bosentan (bos) treatment. Data are presented as ligand (ET1 and bos) activation divided by control treatment activation. Gray line indi- cates a basal activation ratio of 1. *P0.05, †P0.01. B, p38 phos- phorylation/activation induced by CVB3, ET1, and bosentan individu- ally and in combination was determined by phospho–p38 MAPK ELISA over a 30-minute time course (meansSD). *P0.05, ‡P0.001. C, HL1 cells were infected with a GFP-CVB3 and superna- tant was harvested at t0 and 16 hours. Supernatant titer was deter- mined on HeLa cells by flow cytometry. ‡P0.001 as compared to vehicle group.

Article Snippet: Five-week-old A/J mice were infected intraperitoneally with 1.0 105 plaque forming units of CVB3-Gauntt and treated with bosentan and SB203580 HCl (Tocris Pharmaceuticals), starting the following day.

Techniques: Virus, Infection, Activation Assay, Control, Enzyme-linked Immunosorbent Assay, Cytometry

Journal: Circulation Research

Article Title: Bosentan Enhances Viral Load via Endothelin-1 Receptor Type-A–Mediated p38 Mitogen-Activated Protein Kinase Activation While Improving Cardiac Function During Coxsackievirus-Induced Myocarditis

doi: 10.1161/circresaha.108.191171

Figure Lengend Snippet: Figure 5. Phosphorylation of ATF2, a downstream signaling tar- get of p38 MAPK, is increased with bosentan treatment. ATF2 phosphorylation (p-ATF2) in CVB3-infected heart (5 days pi) after vehicle or bosentan treatment was examined by immuno- staining using anti–phospho-ATF2 antibody (brown stain; arrows). Bosentan treatment significantly increases the percent- age of phospho-ATF2 stain (meansSD). ‡P0.001 as com- pared to vehicle control.

Article Snippet: Five-week-old A/J mice were infected intraperitoneally with 1.0 105 plaque forming units of CVB3-Gauntt and treated with bosentan and SB203580 HCl (Tocris Pharmaceuticals), starting the following day.

Techniques: Phospho-proteomics, Infection, Immunostaining, Staining, Control

Journal: Annals of Dermatology

Article Title: Tranexamic Acid Ameliorates Skin Hyperpigmentation by Downregulating Endothelin-1 Expression in Dermal Microvascular Endothelial Cells

doi: 10.5021/ad.23.108

Figure Lengend Snippet: ET-1: endothelin-1, TXA: tranexamic acid, MC: melanocyte, HUVEC: human umbilical vein endothelial cell, CCK-8: cell counting kit-8; qPCR: quantitative polymerase chain reaction, GAPDH: glyceraldehyde 3-phosphate dehydrogenase; SD: standard deviation, CM: conditioned medium, TYR: tyrosinase, MITF: microphthalmia-associated transcription factor, ns: not significant. * p <0.05, ** p <0.01 vs. control.

Article Snippet: Bosentan, an endothelin receptor antagonist for ET-A and ET-B, was purchased from Selleck Chemicals (Cat#: S3051, Houston, TX, USA).

Techniques: CCK-8 Assay, Cell Counting, Real-time Polymerase Chain Reaction, Standard Deviation, Control