Journal: Frontiers in Oncology

Article Title: Characterization of ROS Metabolic Equilibrium Reclassifies Pan-Cancer Samples and Guides Pathway Targeting Therapy

doi: 10.3389/fonc.2020.581197

Figure Lengend Snippet: Drug efficacy can be modulated by targeting reactive oxygen species (ROS) metabolism. (A) The drug efficacies of bortezomib and docetaxel were assessed by adding a low concentration of H 2 O 2 together as assessed by cell proliferation assay. The antitumor efficacy of these two drugs was diminished by exogenous ROS. Error bars indicate the mean ± SD; ns = no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) The drug efficacies of methotrexate and vorinostat were increased by adding a high concentration of H 2 O 2 together as assessed by cell proliferation assay. Error bar indicate the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (C) The tumor inhibitory effects of bortezomib were abolished by exogenous low H 2 O 2 concentrations in a U87 subcutaneous xenograft model; * p < 0.05. Error bars indicate the mean ± SEM. (D) Immuno-histochemical staining for Ki67 shows that the proliferative inhibition of bortezomib was rescued by exogenous low H 2 O 2 concentrations. (E) The NF-κB inhibitory effects of bortezomib were rescued by H 2 O 2 in U87 and LN229 cell lines. (F) Half-dosage of bortezomib could be sensitized by NAC in U87 cell lines; *** p < 0.001. (G) Half-dosage of bortezomib together with NAC could inhibit NF-κB pathway in U87 and LN229 cell lines.

Article Snippet: Bortezomib (MCE, NJ, USA) was administered (0.40 mg/kg) i.p. every 3 days after the animals were assigned to different groups.

Techniques: Concentration Assay, Proliferation Assay, Staining, Inhibition

Journal: bioRxiv

Article Title: Proteostasis Remodeling Across Development Defines Fetal, Neonatal, and Adult Hematopoietic Stem Cell States

doi: 10.64898/2025.12.12.694052

Figure Lengend Snippet: Neonatal HSCs have increased dependence on the proteasome to maintain proteostasis (A-E) Enrichment plots for the “Positive Regulation of Macroautophagy” gene set based on RNA-sequencing data (GSE128759) in HSCs from E16.5 (A), E18.5 (B), P0 (C), P7 (D), and P14 (E) mice compared to 8-week-old mice. (F-J) Enrichment plots for the “Proteasome Assembly” gene set based on RNA-sequencing data in HSCs from E16.5 (F), E18.5 (G), P0 (H), P7 (I), and P14 (J) mice compared to 8-week-old mice. (K-N) Proteasome activity as measured by Proteasome-Glo assay in CD48 - LSK HSCs/MPPs (K), CMPs (L), GMPs (M), and MEPs (N) at E16.5, E18.5, P0, P7, and P14, relative to young adult (7–10-week-old) mice (n = 6-32 sets of 5000 cells from 12-27 mice/age in 3 experiments/age). (O) Relative ubiquitylated protein in HSCs from P7 and 8-week-old mice after injection with 10 mg/kg bortezomib (BTZ) as compared to non-treated age-matched controls (n = 6-11 mice/treatment group in 3-4 experiments/age). Data represent mean ± standard deviation (K-O). Normalized enrichment scores (NES) and false discovery rates (q) are shown in A-J. Statistical significance was assessed using a one-way ANOVA followed by Dunnett’s multiple comparisons test relative to the 7-10-week-old timepoint (K-N) or Welch’s t-tests (O). *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.

Article Snippet: Mice received an intraperitoneal injection of 10 mg/kg bortezomib (Cell Signaling) diluted in 10% (v/v) DMSO.

Techniques: RNA Sequencing, Activity Assay, Glo Assay, Injection, Standard Deviation

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Characterization of the nanoparticles. (A) Measurements of the nanoparticles loaded with bortezomib and prothionamide using dynamic light scattering. The data revealed the intensity distributions before and after ultrasound treatment. (B and C) Transmission electron microscopy images of the PVP-B and PVP-P particles, respectively. PVP, poly(N-vinylpyrrolidone).

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Transmission Assay, Electron Microscopy

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Cytotoxicity of amphiphilic PVP micellar NPs loaded with bortezomib (PVP-B) or prothionamide (PVP-P) in monolayer T98G and U87 human glioblastoma cell lines evaluated by MTT test after 24, 48 or 72 h of incubation. * P<0.05 and ** P<0.01 by Student's t-test. PVP, poly(N-vinylpyrrolidone); NPs, nanoparticles.

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Incubation

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Cytotoxicity of amphiphilic PVP micellar NPs loaded with bortezomib (PVP-B) or prothionamide (PVP-P) in multicellular spheroids of human glioblastoma cells T98G or U87 evaluated by MTT test after 48 or 72 h of incubation. * P<0.05 and ** P<0.01 by Student's t-test. PVP, poly(N-vinylpyrrolidone); NPs, nanoparticles.

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Incubation

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: The morphology of 3D tumor spheroids generated from human glioblastoma cells T98G or U87 after incubation with 500 µg/ml of amphiphilic PVP micellar nanoparticles loaded with bortezomib (PVP-B) or prothionamide (PVP-P) for 48 h or 72 h. The spheroids were visualized using an inverted light microscope. Scale bar, 200 µm. PVP, poly(N-vinylpyrrolidone).

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Generated, Incubation, Light Microscopy

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Cytotoxicity of amphiphilic PVP micellar NPs loaded with bortezomib (PVP-B) or prothionamide (PVP-P) in normal human fibroblasts after 72 h of incubation evaluated by MTT test. * P<0.05 by Student's t-test. PVP, poly(N-vinylpyrrolidone); NPs, nanoparticles.

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Incubation

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Zebrafish Danio rerio embryos after 24-h (48 hpf) and 72-h (96 hpf) incubation with bortezomib-loaded amphiphilic PVP micellar nanoparticles (PVP-B). Arrows indicate developmental delays. Х indicates embryos' death. The images were captured using a Nexcope NSZ-810 microscope at x40 magnification. hpf, hours post-fertilization; PVP, poly(N-vinylpyrrolidone).

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Incubation, Microscopy

Journal: Biomedical Reports

Article Title: Assessment of the effects of amphiphilic poly (N‑vinylpyrrolidone) nanoparticles loaded with bortezomib on glioblastoma cell lines and zebrafish embryos

doi: 10.3892/br.2024.1725

Figure Lengend Snippet: Survival of Danio rerio embryos (n=6 per group) during 24-h (48 hpf) and 72-h (96 hpf) incubation with bortezomib-loaded amphiphilic PVP micellar nanoparticles PVP-B. Lethal concentration 50% equals to 0.1 mg/ml of PVP-B and was determined by regression analysis using GraphPad Prism version 8.01. hpf, hours post-fertilization; PVP, poly(N-vinylpyrrolidone).

Article Snippet: Bortezomib was obtained from Santa Cruz Biotechnology, Inc. Dulbecco's Modified Eagle Medium (DMEM), trypsin-versene solution and PBS were purchased from PanEco.

Techniques: Incubation, Concentration Assay