Journal: Poultry Science

Article Title: Effect of rearing environment on bone growth of pullets 1

doi: 10.3382/ps/peu041

Figure Lengend Snippet: Effect of age and housing systems in systemic markers of bone formation and resorption in Lohmann White pullets housed in AV system and CCs: A = serum osteocalcin concentration; B = serum pyridinoline concentration.

Article Snippet: Serum osteocalcin (marker of bone formation) and hydroxylysyl pyridinoline (marker of bone resorption) concentrations were quantified using commercially available ELISA tests (Quidel Corporation, San Diego, CA).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking

doi: 10.3390/ijms21228581

Figure Lengend Snippet: Inhibition of induction of carbonic anhydrase II (CAII), anion exchange protein 2 (Ae2), chloride channel 7 (ClC-7), vacuolar-type H(+)-ATPase (V-ATPase), and cathapsin K ( A , B ), matrix metalloproteinase 9 (MMP-9) secretion ( C ), and bone resorption ( D ) by aesculetin. Raw 264.7 cells were cultured in minimum essential medium alpha medium (α-MEM) with 50 ng/mL receptor activator of nuclear factor-κB ligand (RANKL) in the absence or presence of 1–10 μM aesculetin for 5 days. Whole-cell lysates were subject to SDS-PAGE and Western blot with a specific antibody against CAII, Ae2, ClC-7 V-ATPase, and cathapsin K ( A , B ). An equal volume of culture medium was subject to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot with a specific antibody against MMP-9 ( C ). β-Actin was used as internal control. The bar graphs (mean ± SEM, n = 3) represent quantitative results of blots obtained from a densitometer. Respective values in double-bar graphs not sharing a small letter are significantly different at p < 0.05. The osteoclast bone resorption was assayed using a commercially available bone resorption assay kit (( D ), three separate experiments). Attached cells were removed, and resorption pits on the plate were visualized under light microscopy. Scale bar = 20 µm.

Article Snippet: The bone resorption assay was performed using a resorption assay kit (CSR-BRA-48X2KIT, Cosmo Bio Co., Tokyo, Japan).

Techniques: Inhibition, Cell Culture, SDS Page, Western Blot, Polyacrylamide Gel Electrophoresis, Control, Light Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking

doi: 10.3390/ijms21228581

Figure Lengend Snippet: Schematic diagram showing the inhibitory effects of aesculetin on ruffled border formation and lysosomal trafficking responsible for bone resorption. As depicted, aesculetin blocks the RANKL signaling process responsible for bone resorption. The symbol I − indicates sites of inhibition manifested by aesculetin.

Article Snippet: The bone resorption assay was performed using a resorption assay kit (CSR-BRA-48X2KIT, Cosmo Bio Co., Tokyo, Japan).

Techniques: Inhibition

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Open Bristow Versus Open Latarjet for Anterior Shoulder Instability in Rugby Players: Radiological and Clinical Outcomes

doi: 10.1177/23259671221095094

Figure Lengend Snippet: Classification of postoperative bone resorption of the coracoid on computed tomography images with multiplanar reconstruction. (A) None: No bone resorption. (B) Mild: Slight bone resorption, but it did not reach the screw head. (C) Moderate: Bone resorption reached the screw head. (D) Severe: Severe bone resorption with exposure of the screw shaft and loosening of the washer.

Article Snippet: In the Bristow group, bone resorption was observed in 4 shoulders (6%) at 6 months after surgery.

Techniques: Computed Tomography

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Open Bristow Versus Open Latarjet for Anterior Shoulder Instability in Rugby Players: Radiological and Clinical Outcomes

doi: 10.1177/23259671221095094

Figure Lengend Snippet: Representative cases of bone resorption after the Bristow procedure. (A) Axial computed tomography (CT) views of the right shoulder of a 16-year-old boy showing mild bone resorption of the articular side of the coracoid. (B) Sagittal CT views of the left shoulder of a 17-year-old boy showing moderate bone resorption of the coracoid tip.

Article Snippet: In the Bristow group, bone resorption was observed in 4 shoulders (6%) at 6 months after surgery.

Techniques: Computed Tomography

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Open Bristow Versus Open Latarjet for Anterior Shoulder Instability in Rugby Players: Radiological and Clinical Outcomes

doi: 10.1177/23259671221095094

Figure Lengend Snippet: Rate of Postoperative Bone Resorption of the Coracoid After the Latarjet Procedure a

Article Snippet: In the Bristow group, bone resorption was observed in 4 shoulders (6%) at 6 months after surgery.

Techniques:

Journal: Orthopaedic Journal of Sports Medicine

Article Title: Open Bristow Versus Open Latarjet for Anterior Shoulder Instability in Rugby Players: Radiological and Clinical Outcomes

doi: 10.1177/23259671221095094

Figure Lengend Snippet: Representative cases of bone resorption after the Latarjet procedure on 3-dimensional computed tomography images (axial view on the left and sagittal view on the right of each image pair). (A) Left shoulder of a 17-year-old boy at (A) 1 day after surgery and (A′) 1 year after surgery showing bone resorption at both superior and inferior sides of the coracoid process. (B) Right shoulder of a 16-year-old boy at (B) 1 day after surgery and (B′) 1 year after surgery showing bone resorption at superior and articular sides of the coracoid process.

Article Snippet: In the Bristow group, bone resorption was observed in 4 shoulders (6%) at 6 months after surgery.

Techniques: Computed Tomography