Journal: Materials Today Bio

Article Title: Pressure-sensitive composite bandages with high toughness, self-healability and strong tissues adhesion for rapid bone fixation and accelerated osteogenesis via immunomodulation and neovascularization

doi: 10.1016/j.mtbio.2025.102730

Figure Lengend Snippet: In vitro osteogenic capacity of POC-G/HA bone adhesives. (A) ALP staining and (C) ARS staining of rBMSCs cultured with POC-G/HA adhesives. Quantitative analysis of ALP activity (B) and ARS staining (D). RT-qPCR results of ALP (E), Runx-2 (F), and BMP2 (G) for detecting relative osteogenic genes expression after 14 days incubation. Results were normalized with GAPDH. (n = 3). Western blot analysis of ALP (H), Runx-2 (I) and BMP2 (J) for detecting osteogenic-related proteins expression (n = 3). Statistical significance: ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001 (ANOVA).

Article Snippet: Transcript levels of ALP , BMP2 , and Runx-2 were measured using SYBR Premix Ex Taq II (primer sequences in ), with GAPDH (Proteintech, USA) serving as the reference gene.

Techniques: In Vitro, Staining, Cell Culture, Activity Assay, Quantitative RT-PCR, Expressing, Incubation, Western Blot

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Stroke Alters the Function of Enteric Neurons to Impair Smooth Muscle Relaxation and Dysregulates Gut Transit

doi: 10.1161/JAHA.123.033279

Figure Lengend Snippet: The CX3CR1 + muscularis macrophage (MM) population of the small intestines of sham‐ and permanent midcerebral occlusion (pMCAO)‐operated mice at 24 hours was isolated and their transcriptional profile was determined via RNA sequencing. ( A ) Volcano plot showing statistically significant genes in red (false discovery rate of ≤0.05, absolute log fold change=1, Limma‐voom; n =17 for each group. Samples were pooled to obtain >7000 cells per sample to maximize the extracted RNA quality and quantity for sequencing). ( B ) Fold change (FC) of differentially expressed genes after stroke relative to sham in CX3CR1 + MMs. FC values are represented in the log 2 scale. ( C ) STRING analysis of protein networks, whereby the network edges are the interaction evidence with medium confidence (0.400). The expression of major histocompatibility complex (MHC) II on MMs ( D ) and median value of the mean fluorescent intensity (MFI; E ) were quantified using flow cytometry (n=8 for the sham group; n=7 for the pMCAO group). ( F ) Fold change of neuron–macrophage crosstalk genes in the muscle layer of pMCAO group relative to sham (dotted line) was analyzed via BioMark quantitative polymerase chain reaction (n=8 per group). ( G ) Quantification of bone morphogenetic protein 2 (BMP2) concentration (pg/g of tissue) in the muscle layer via an ELISA. Each data point represents one animal. Data are shown as mean±SEM. *** P <0.001 (unpaired 2‐tailed Student's t test; n=8 per group). BMP2, bone morphogenetic protein; ELISA, enzyme‐linked immunosorbent assays; FC, fold change; MFI, mean fluorescent intensity; MM, muscularis macrophage; PGP9.5, protein gene product 9.5; pMCAO, permanent middle cerebral occlusion; RNA, ribonucleic acid; ROI, region of interest.

Article Snippet: The concentration of BMP2 in the muscularis externa of the whole small intestine was determined using the Mouse BMP2 ELISA Kit PicoKine (EK0313; Boster Biological Technology, Pleasanton, CA) as per manufacturer's protocol.

Techniques: Isolation, RNA Sequencing, Sequencing, Expressing, Immunopeptidomics, Flow Cytometry, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay