Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Colorimetric staining and quantification of ALP activity in hBM-MSCs cultured for seven days on collagen type I-coated (a) and fibronectin-coated (b) substrates. Results are expressed as mean ± SD (n=3). Data were analyzed assuming a Gaussian (normal) distribution and equal variances across groups; statistical differences were assessed using one-way ANOVA. Only p-values <0.1 are shown. Scale bar represents 200 µm.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Staining, Activity Assay, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Fluorescence images of hBM-MSCs cultured on β-PVDF films coated with either collagen type I (a) or fibronectin (b) and corresponding morphological features. Nuclei are stained in blue, vinculin in green and F-actin in red. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown. Scale bars represent 200 µm.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Fluorescence, Cell Culture, Staining

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: MYPT1 phosphorylation in hBM-MSCs cultured on collagen type I-coated (a) and fibronectin-coated (b) β-PVDF films of varying surface potential. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Phospho-proteomics, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Fluorescence images of hBM-MSCs cultured on β-PVDF films coated with either collagen type I (a) or fibronectin (b) immunostained against YAP and counterstained with Hoechst 33342, and the corresponding quantifications (right panels). Scale bars represent 200 µm. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Fluorescence, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Volcano plots with the −log 10 (p-value) plotted against their respective log 2 (fold change) of genes differentially expressed in hBM-MSCs, Venn diagrams and histogram plots showing genes that are down- or upregulated (p<0.05) in hBM-MSCs cultured on the indicated surfaces for 24 hours (a) or four days (b). Circle area in a and b is proportional to the number of genes. GSEA of the cells cultured on the different surfaces for 24 hours (c) and 4 days (d).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: (a) Immunostaining of hBM-MSCs cultured on the different β-PVDF surfaces with glutaraldehyde-crosslinked collagen type I coating and corresponding morphologic analysis (b). MYTP1 phosphorylation (c) and YAP translocation (d and e) in hBM-MSCs cultured on the substrates. Only p-values <0.1 are shown. Scale bars represent 200 µm. Results are expressed as mean ± SD (n=3).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Immunostaining, Cell Culture, Phospho-proteomics, Translocation Assay

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Immunofluorescent images (a) and corresponding morphologic analysis (b) of hBM-MSCs treated with the ROCK inhibitor Y-27632 for 24 hours. YAP immunolocalization (c) and translocation (d) in treated cells. Only p-values <0.1 are shown. Scale bars represent 300 µm. Results are expressed as mean ± SD (n=4).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Translocation Assay

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: A schematic illustration of the preparation of MSCs-laden gelatin methacrylate/silk fibroin (GelMA/SF, SG) hydrogel with the incorporation of PRP for treating KOA to reconstruct cartilage.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques:

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The MSCs proliferation after loaded in SG hydrogels and cultured for 72 h, cells cultured without hydrogel as a control group. Laser scanning confocal microscope (LSCM) images of MSCs loaded in SG-1 ( A , B , and C ), SG-2 (D, E, and F), and SG-3 ( G , H , and I ) hydrogels, and without hydrogels ( J , K , and L ) cultured for 72 h, images ( A , D , G , and J ) displayed that cell nucleus stained using DAPI as shown in blue color, images ( B , E , H , and K ) displayed that cytoskeleton stained using Phalloidin-FITC as shown in green color, and image ( C , F , I , and L ) was merged from DAPI, and Phalloidin-FITC stained images, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture, Control, Microscopy, Staining

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Cell viability of MSCs after loaded in SG hydrogels and cultured for different days. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Optical micrographs of ( A-H ) H&E and ( I–P ) Masson staining slices of tissues after SG hydrogels ( C, D, K, and L ), SG hydrogels incorporated with PRP ( E, F, M, and N ), and MSCs-laden SG hydrogels incorporated with PRP ( G, H, O, and P ) were intra-articular injected into SD rats for 8 weeks, respectively, the SD rats without hydrogels intervention as the control group ( A, B, I, and J ).

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Staining, Injection, Control

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The obtained scores for SG hydrogel, SG hydrogel with PRP, MSCs-loaded hydrogel with PRP, and control group using Mankin Scoring system. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Control

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Scanning electron microscopy characterization of mesenchymal stem cell attachment and proliferation on three biomaterial compositions. Top panels ( A – C ): Biomaterial surfaces prior to cell seeding, showing the characteristic surface morphology of Group 1 (PCL + HA, 60/40) ( A ), Group 2 (PCL + PLGA, 50/50) ( B ), and Group 3 (PCL + PLGA + HA, 30/30/40) ( C ) respectively. Bottom panels ( D – F ): Mesenchymal stem cells after 21 days of culture in osteogenic differentiation medium, demonstrating robust cell attachment, spreading, and surface colonization on Groups 1 ( D ), 2 ( E ), and 3 ( F ), respectively. Panel ( G ): Higher magnification view (×3500) illustrating characteristic elongated morphology of healthy, proliferative mesenchymal stem cells with extensive filopodia and lamellipodia formation extending across the Group 3 biomaterial surface, indicating strong cell–substrate interactions and active synthetic activity.

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Electron Microscopy, Cell Attachment Assay, Activity Assay

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Alamar Blue assay evaluating mesenchymal stem cell metabolic activity on three 3D-printed biomaterial compositions over time. ( A ) Line graph showing percentage of cell metabolic activity measured at days 3, 7, 14, and 21 on PCL + HA (60/40), PCL + PLGA (50/50), and PCL + PLGA + HA (30/30/40) substrates. Days 3, 7, 14, and 21 refer to the duration of osteogenic differentiation culture, defined as Day 0 of osteogenic induction initiated after 3 days of proliferation medium pre-culture. ( B ) Corresponding bar graph representation of metabolic activity for each composition at individual time points (D3, D7, D14, D21). Data are presented as mean ± SEM (n = 3 biological replicates per group per timepoint). Statistical annotations indicate comparisons between material groups as follows: * comparison between PCL + PLGA + HA and PCL + HA; # comparison between PCL + PLGA + HA and PCL + PLGA in A. Normality of residuals was assessed using the Shapiro–Wilk test, and homogeneity of variances was assessed using Levene’s test; assumptions were met for all groups (Shapiro p > 0.05; Levene p > 0.05). Statistical differences were evaluated by two-way ANOVA, followed by Tukey multiple comparisons with Bonferroni correction.#: PCL + PLGA + HA vs. PCL + PLGA (# p < 0.05, ## p < 0.01, (n = 3 per group)) *: PCL + PLGA + HA vs. PCL + HA ( p < 0.05 (*), p < 0.01 (**), p < 0.001 (***)).

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Alamar Blue Assay, Activity Assay, Comparison

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Relative gene expression of osteogenic markers in MSCs cultured on Implant A and Implant B 3D-printed PCL + PLGA + HA (30/30/40) composite scaffolds at days 14 and 21 of osteogenic differentiation. Days 14 and 21 refer to the duration of osteogenic differentiation culture, initiated after 3 days of proliferation medium pre-culture. Implant A: surface-coated PCL + PLGA + HA (30/30/40); Implant B: uncoated PCL + PLGA + HA (30/30/40) control. ( A ) Alkaline phosphatase (ALP), ( B ) Runt-related transcription factor 2 (RUNX2), and ( C ) Bone gamma-carboxyglutamate protein (BGLAP). Gene expression was normalized to GAPDH using the 2 −ΔΔCt method. Data are presented as mean ± SD (n = 4 biological replicates, each analyzed with three technical replicates). Normality of residuals was assessed using the Shapiro–Wilk test, and homogeneity of variances was assessed using Levene’s test; assumptions were met for all genes (Shapiro p > 0.05; Levene p > 0.05). Statistical differences were evaluated gene-by-gene using a two-way ANOVA (Time_Point: Day 14 vs. Day 21; Condition: Implant A vs. Implant B) including the interaction term (Time_Point × Condition), followed by Tukey/emmeans post hoc multiple comparisons with Bonferroni correction. Significance is indicated as follows: p < 0.05 (*) and p < 0.001 (***).

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Gene Expression, Cell Culture, Control

Journal: Current Research in Pharmacology and Drug Discovery

Article Title: Role of pro-inflammatory cytokines in Alzheimer's disease and neuroprotective effects of pegylated self-assembled nanoscaffolds

doi: 10.1016/j.crphar.2022.100149

Figure Lengend Snippet: Effect of Memp, Marketed, (MEM-PLGA) SANs, (PEG-MEM-PLGA) SANs and (PEG-MEM-PLGA)-BMSc SANs on pro-inflammatory cytokines (a) IL-1β (b) IL-6 (c) IL-10 and (d) TNF-α observed in serum, cerebral, hepatic and renal tissues of treatment groups of scopolamine induced AD model vs. control and disease control (untreated cells) group and Data are represented as Mean ​± ​SEM, (n ​= ​3).

Article Snippet: HighQCTM Human Bone Marrow Derived Stem Cell (BMSc) obtained from ACCEGEN BIOTECHNOLOGY were grown in Minimum Essential Media (MEM).

Techniques: Control