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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Boule Medical AB haematology analyser medonic m-series
Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Boule Medical AB mixer 820
Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Boule Medical AB exigo eos veterinary hematology analyzer
Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification <t>of</t> <t>spermatogonia</t> and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and <t>BOLL</t> expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
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Image Search Results


Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification of spermatogonia and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and BOLL expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.

Journal: Human Reproduction (Oxford, England)

Article Title: Complete spermatogenesis in intratesticular testis tissue xenotransplants from immature non-human primate

doi: 10.1093/humrep/dey373

Figure Lengend Snippet: Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification of spermatogonia and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and BOLL expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.

Article Snippet: Following another washing step, non-specific adhesion sites were blocked with 4% normal goat serum (Tebu-bio, Boechout, Belgium) diluted in PBS or with Cas-block (Life Technologies) (only for CREM-1 antibody) for 30 min. Next, primary antibodies against VIMENTIN (marmoset specific), MAGE-A4 (mouse monoclonal anti-melanoma-associated antigen 4; dilution 1:200; gift from Dr Spagnoli; marker for spermatogonia and primary spermatocytes, marmoset specific), BOLL (mouse monoclonal anti-BOLL; dilution 1:400; H00066037-M03; R&D system, Abingdon, UK; marker for spermatocytes (meiotic cells)), CREM-1 (rabbit polyclonal anti-CREM-1; dilution 1:400; sc-440; Santa-Cruz Biotechnology, Heidelberg, Germany; marker for round spermatids (post-meiotic cells)) and ACROSIN (rabbit polyclonal anti-ACROSIN; dilution 1:500; sc-67151; Santa-Cruz; marker for acrosome visualisation in round, elongating and elongated spermatids (post-meiotic cells), marmoset specific), were applied to the sections and incubated in a humidified chamber overnight at 4°C.

Techniques: Cell Differentiation, Transplantation Assay, Immunostaining, Expressing, Marker, Membrane