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Image Search Results
Journal: Human Reproduction (Oxford, England)
Article Title: Complete spermatogenesis in intratesticular testis tissue xenotransplants from immature non-human primate
doi: 10.1093/humrep/dey373
Figure Lengend Snippet: Germ cell differentiation in ectopic marmoset testis tissue xenotransplants at 4 months post-transplantation . Morphological identification of spermatogonia and spermatocytes by H/PAS and VIMENTIN, and confirmation by immunostaining for MAGE-A4 and BOLL expression. Transplants were negative for the post-meiotic marker CREM-1. Arrows indicate spermatocytes in the lumen of marmoset seminiferous tubules; arrowheads indicate spermatogonia at the basal membrane.
Article Snippet: Following another washing step, non-specific adhesion sites were blocked with 4% normal goat serum (Tebu-bio, Boechout, Belgium) diluted in PBS or with Cas-block (Life Technologies) (only for CREM-1 antibody) for 30 min. Next, primary antibodies against VIMENTIN (marmoset specific), MAGE-A4 (mouse monoclonal anti-melanoma-associated antigen 4; dilution 1:200; gift from Dr Spagnoli; marker for spermatogonia and primary spermatocytes, marmoset specific),
Techniques: Cell Differentiation, Transplantation Assay, Immunostaining, Expressing, Marker, Membrane