Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis.

doi: 10.1002/advs.202416419

Figure Lengend Snippet: Figure 3. Activated HSCs enhance fatty acid (FA) synthesis and transfer to cancer cells, promoting proliferation. A) Western blot showing the ACC1 and CPT1A level of Huh7 cells and Huh7 cells cocultured with aHSCs (n = 3). B) Schematic of the experimental setup for FA chase pulse assay. HSCs were incubated in complete media (CM) with red fluorescent FA (Red C12) for 16 h (“pulse”). Following this, HSCs were cultured for an additional 72 h in CM or tumor-conditioned media (TCM) without the labeled FA (“chase”). Mitochondria and lipid drops (LDs) were stained before imaging. C,D) FA localization was assayed as described in (A) and chased in CM or TCM. C) LDs were labeled using BODIPY 493/503 (gray) and mitochondria were labeled using MitoTracker Far Red (green). Scale bar = 25 μm. D) Relative cellular localization of Red C12 was quantified by Pearson’s coefficient analysis

Article Snippet: For FA transfer assays, HSCs were preloaded with 1 × 10−3 m Red C12 (BODIPY 558/568 C12, MCE, Catalog No. HY-138226) in CM for 16 h, then rigorously washed three times with CM and equilibrated for 1 h to remove extracellular dye aggregates.

Techniques: Western Blot, Incubation, Cell Culture, Labeling, Staining, Imaging

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis.

doi: 10.1002/advs.202416419

Figure Lengend Snippet: Figure 5. Complementary roles of FAs transfer and mitochondrial transport in supporting tumor cell survival. A) Representative confocal images of Huh7 cells cocultured with aHSCs under L-778123 and NTL treatment. Maximum intensity projections (MIP) of confocal Z-stacks showing Huh7 cells (CellTrace green, green) cocultured with aHSCs pre-labeled with Red C12 fluorescent FA (red) (Upper panels). Corresponding 3D surface renderings (Imaris) of the boxed regions (Lower panels). Scale bars: 15 μm. B) Graph showing the fluorescence intensity of FAs in CellTrace green labeled Huh7 cells (Data are represented as mean ± SD, n = 6, at least 50 recipient cells analyzed per replicate, one-way ANOVA was performed). C) Flow cytometry analysis of fluorescence intensity of FAs in FITC-positive Huh7 cells. D) Confocal images showing the transportation of mitochondria from aHSCs to Huh7 cells. (Upper panels) MIP: Confocal Z-stacks of cocultured MitoTracker-labeled mitochondria in aHSCs (red) and CellTrace green-labeled Huh7 cells (green), with ActinTracker (cyan) highlighting tunneling nanotubes (TNTs). (Lower panels) 3D surface reconstruction (Imaris). Scale bars: 15 μm. E) Quantification of fluorescence intensity from Figure D, showing the uptake of HSC-derived mitochondria by Huh7 cells (Data are represented as mean ± SD, n = 6, at least 50 recipient cells analyzed per replicate, one-way ANOVA was performed). F) Fluorescence histograms of Huh7 cells (labeled

Article Snippet: For FA transfer assays, HSCs were preloaded with 1 × 10−3 m Red C12 (BODIPY 558/568 C12, MCE, Catalog No. HY-138226) in CM for 16 h, then rigorously washed three times with CM and equilibrated for 1 h to remove extracellular dye aggregates.

Techniques: Labeling, Flow Cytometry, Derivative Assay, Fluorescence

Journal: Structure (London, England : 1993)

Article Title: The RNA-Binding Site of Poliovirus 3C Protein Doubles as a Phosphoinositide-Binding Domain

doi: 10.1016/j.str.2017.11.001

Figure Lengend Snippet:

Article Snippet: GloPIPs BODIPY® FL-PI(3,4,5)P 3 , Echelon Biosciences , C-39F6a.

Techniques: Virus, Recombinant, Protease Inhibitor, Clone Assay, Mutagenesis, Plasmid Preparation, Software

Journal: Experimental & Molecular Medicine

Article Title: Cholesterol biosynthesis induced by radiotherapy inhibits cGAS–STING activation and contributes to colorectal cancer treatment resistance

doi: 10.1038/s12276-025-01457-6

Figure Lengend Snippet: a Plot of the orthogonal projections to latent structures discriminant analysis (OPLS-DA) score for metabolomics in the two groups. b Volcano plots of the metabolomics with significant changes after radiotherapy. c Bar chart of metabolite sets enrichment analysis (MSEA) of differential metabolites in the two groups. d The top 20 significantly enriched pathways in GO terms for biological process (BP). e Heatmap of log 2 fold change (FC) to depict the gene expression associated with cholesterol biosynthesis. f GSEA of cholesterol biosynthetic process (GO: 0006695) between the two groups. g The cholesterol levels in CT26 tumor tissues on the 10th day after local 6 Gy irradiation. h The cholesterol levels in CT26 and HCT116 cells at 24 h after exposure to 0, 2, 4 and 6 Gy irradiation. i The cholesterol levels in CT26 and HCT116 cells at different times (0, 12, 24, 48 and 72 h) after exposure to 6 Gy irradiation. j , k At 24 h after exposure to 0, 2, 4 and 6 Gy, CT26 ( j ) and HCT116 cells ( k ) were labeled with Hoechst (blue) and cholesterol-BODIPY (green). Scale bar, 10 μm. ** P < 0.01; * P < 0.05; ns, not significant.

Article Snippet: Cholesterol-boron difluoride dipyrrin complex (BODIPY) (cat. no. HY-125746), water-soluble cholesterol (cat. no. HY-N0322A) and Filipin III (cat. no. HY-N6718) were obtained from MedChemExpress.

Techniques: Gene Expression, Irradiation, Labeling