Journal: International Journal of Obesity (2005)

Article Title: Epac2a-null mice exhibit obesity-prone nature more susceptible to leptin resistance

doi: 10.1038/ijo.2016.208

Figure Lengend Snippet: Effect of S220 on high-fat diet-induced obesity. ( a ) Representative western blot data and the quantification of hypothalamic pSTAT3 (Y705) protein expression in normal chow diet, 7-week-old WT mice. Mice i.p. received S220 (20 mg kg −1 ) or saline 30 min prior to leptin (5 mg kg −1 ) or PBS injection. Sampling was achieved after 45 min. The experiments were repeated at least three times. Daily food intake ( b ) and changes in body weight ( c ) in WT mice during 6 weeks of high-fat diet (HFD) feeding. Five mice were allocated randomly for each group. Daily S220 or PBS injection started from 2 weeks after HFD feeding. ( d ) Fasting plasma leptin levels measured after 4-week treatment with S220 or PBS on a HFD ( n =5). The values represent means±s.e.m. * P <0.05 vs Chow diet WT, # P <0.05 vs HFD WT with PBS.

Article Snippet: The selective Epac2 activator S220 (BIOLOG, Bremen, Germany) was tested on diet-induced obesity.

Techniques: Western Blot, Expressing, Saline, Injection, Sampling, Clinical Proteomics

Journal: Vaccines

Article Title: Long-Term Antibody Response against SARS-CoV-2 in Health Care Workers: Effectiveness of Homologous and Heterologous Regimens and Their Relation to Systemic Vaccine-Associated Symptoms

doi: 10.3390/vaccines10101599

Figure Lengend Snippet: Outline of the study flow illustrating the timeline for recruiting participants according to vaccine regimens. Green: BNT/BNT, the BNT162b2 mRNA vaccine from Pfizer/BioNTech. Blue: m1273/m1273, the mRNA-1273 vaccine from Moderna. Purple: ChAd/m1273, the heterologous vaccine regimen combining ChAdOx1 vaccine from AstraZeneca with m1273 from Moderna. Early and late measurement refers to antibody level on average one and six months after completion of a vaccine regimen with a second vaccine dose, respectively.

Article Snippet: We provide evidence that both the effectiveness and durability of the humoral immune responses induced by the heterologous off-label AstraZeneca/Moderna (ChAd/m1273) vaccine regimen is at least equivalent to the homologous standard vaccine regimens with either Pfizer (BNT/BNT) or Moderna (m1273/m1273) used in Denmark.

Techniques:

Journal: Vaccines

Article Title: Long-Term Antibody Response against SARS-CoV-2 in Health Care Workers: Effectiveness of Homologous and Heterologous Regimens and Their Relation to Systemic Vaccine-Associated Symptoms

doi: 10.3390/vaccines10101599

Figure Lengend Snippet: Concentration of antibodies against SARS-CoV-2 spike (S) protein in plasma at late and early measurements according to vaccine regimen. Results are presented as box plots, where the ends of the boxes define the 25th and 75th percentiles and with a line at the median. Individual values for each vaccine group appear as green circles (BNT/BNT, the BNT162b2 mRNA vaccine from Pfizer/BioNTech), blue squares (m1273/m1273, the mRNA-1273 vaccine from Moderna), and purple diamonds (ChAd/m1273, the heterologous vaccine regimen combining ChAdOx1 vaccine from AstraZeneca with m1273 from Moderna). Individuals with paired measurements (both early and late antibody measurements in the same person) are marked with a line combining the two measurements. N is the number of individuals in each vaccine group and N-paired is the number of individuals with paired measurements. The red circle indicates one participant with a PCR-confirmed infection with SARS-CoV-2 between early and late tests. Interquartile range (IQR) indicates the difference between the 75th and 25th percentile.

Article Snippet: We provide evidence that both the effectiveness and durability of the humoral immune responses induced by the heterologous off-label AstraZeneca/Moderna (ChAd/m1273) vaccine regimen is at least equivalent to the homologous standard vaccine regimens with either Pfizer (BNT/BNT) or Moderna (m1273/m1273) used in Denmark.

Techniques: Concentration Assay, Infection

Journal: Vaccines

Article Title: Long-Term Antibody Response against SARS-CoV-2 in Health Care Workers: Effectiveness of Homologous and Heterologous Regimens and Their Relation to Systemic Vaccine-Associated Symptoms

doi: 10.3390/vaccines10101599

Figure Lengend Snippet: Concentration of antibodies against SARS-CoV-2 spike (S) protein in plasma after completion of the vaccine regimen with the second dose of vaccine. Green circles: BNT/BNT, two doses BNT162b2 mRNA vaccine from Pfizer/BioNTech). Blue squares: m1273/m1273, two doses mRNA-1273 vaccine from Moderna. Purple diamonds: ChAd/m1273, the heterologous vaccine regimen combining ChAdOx1 vaccine from AstraZeneca with m1273 from Moderna. Paired measurements are marked with lines. The individual marked with red had a PCR-confirmed infection with SARS-CoV-2 between the early and late antibody measurements.

Article Snippet: We provide evidence that both the effectiveness and durability of the humoral immune responses induced by the heterologous off-label AstraZeneca/Moderna (ChAd/m1273) vaccine regimen is at least equivalent to the homologous standard vaccine regimens with either Pfizer (BNT/BNT) or Moderna (m1273/m1273) used in Denmark.

Techniques: Concentration Assay, Infection

Journal: Vaccines

Article Title: Long-Term Antibody Response against SARS-CoV-2 in Health Care Workers: Effectiveness of Homologous and Heterologous Regimens and Their Relation to Systemic Vaccine-Associated Symptoms

doi: 10.3390/vaccines10101599

Figure Lengend Snippet: Concentration of antibodies against SARS-CoV-2 spike (S) protein in plasma 5 to 7 months after completion of vaccine regimen with the second dose of vaccine according to vaccine regimen and vaccine-associated symptoms (VAS). Results are presented as both individual values and as box plots, where the ends of the boxes define the 25th and 75th percentiles, and with a line at the median. Green circles: BNT/BNT, the BNT162b2 mRNA vaccine from Pfizer/BioNTech. Blue squares: m1273/m1273, the mRNA-1273 vaccine from Moderna. Purple diamonds: ChAd/m1273, the heterologous vaccine regimen combining ChAdOx1 vaccine from AstraZeneca with m1273 from Moderna. None refers to no VAS; Local refers to local VAS, and Systemic to systemic VAS. Interquartile range (IQR) indicates the difference between the 75th and 25th percentile.

Article Snippet: We provide evidence that both the effectiveness and durability of the humoral immune responses induced by the heterologous off-label AstraZeneca/Moderna (ChAd/m1273) vaccine regimen is at least equivalent to the homologous standard vaccine regimens with either Pfizer (BNT/BNT) or Moderna (m1273/m1273) used in Denmark.

Techniques: Concentration Assay

Journal: Annals of Medicine

Article Title: Neutralizing antibodies against SARS-CoV-2 of vaccinated healthcare workers in Taiwan

doi: 10.1080/07853890.2024.2442533

Figure Lengend Snippet: Neutralizing antibody levels between groups and post-vaccination days. Analysis of neutralizing antibody levels using the cPass SARS-CoV2 neutralizing antibody assay after 2 doses of vaccination. (A) Comparison of neutralizing antibody levels between 3 groups of participants who received the vaccine (1st and 2nd dose of vaccine combinations) from AstraZeneca (AZD1222) and Moderna (mRNA-1273) vaccines. (B) Longitudinal analysis of the correlation between neutralizing antibody levels and post-vaccination days who received the AZD1222 + AZD1222 vaccine group (blue color). (C). Longitudinal analysis of the correlation between neutralizing antibody levels and post-vaccination days who received the AZD1222 + mRNA-1273 vaccine group(green color). (D) Longitudinal analysis of the correlation between neutralizing antibody levels and post-vaccination days who received the mRNA-1273 + mRNA1273 vaccine group (red color). The difference in neutralizing antibody levels between groups was analyzed using the Kruskal-Wallis test (ns: not significant and **** p < 0.0001). Data were expressed as median and IQR. The Pearson correlation was used for the analysis of the correlation between neutralizing antibody levels and post-vaccination days.

Article Snippet: A study in Thailand with other mRNA vaccine boosters (Pfizer-BioNTech/BNT) also shows higher antibody levels than AZD1222 boosters in healthcare workers [ ].

Techniques: Comparison, Vaccines

Journal: Annals of Medicine

Article Title: Neutralizing antibodies against SARS-CoV-2 of vaccinated healthcare workers in Taiwan

doi: 10.1080/07853890.2024.2442533

Figure Lengend Snippet: Neutralizing antibody levels and age. Correlation of antibody levels and age, measured using cPass SARS-CoV-2 neutralizing antibody assay after 2 doses of vaccination with age: (A) AZD1222 + AZD1222 vaccine group (blue color). (B) AZD1222 + mRNA-1273 vaccine group (green color). (C) mRNA-1273 + mRNA-1273 vaccine group (red color). The Pearson correlation was used for the analysis of the correlation between neutralizing antibody levels and age.

Article Snippet: A study in Thailand with other mRNA vaccine boosters (Pfizer-BioNTech/BNT) also shows higher antibody levels than AZD1222 boosters in healthcare workers [ ].

Techniques:

Journal: Biomedicine & Pharmacotherapy

Article Title: Reactogenicity and immunogenicity of heterologous prime-boost immunization with COVID-19 vaccine

doi: 10.1016/j.biopha.2022.112650

Figure Lengend Snippet: Summary of included studies on heterologous COVID-19 vaccination.

Article Snippet: , In these two studies, the mRNA was either BNT and mRNA1273from Moderna , Schmidt cellular , Humoral response , In transplant recipients, the heterologous regimen led to significant induction in IgG level and neutralizing activity , , , , .

Techniques:

Journal: Biomedicine & Pharmacotherapy

Article Title: Reactogenicity and immunogenicity of heterologous prime-boost immunization with COVID-19 vaccine

doi: 10.1016/j.biopha.2022.112650

Figure Lengend Snippet: Immunogenicity of heterologous regimens including vector-based and mRNA-based vaccines (ChAd/BNT or ChAd/mRNA-1273).

Article Snippet: , In these two studies, the mRNA was either BNT and mRNA1273from Moderna , Schmidt cellular , Humoral response , In transplant recipients, the heterologous regimen led to significant induction in IgG level and neutralizing activity , , , , .

Techniques: Immunopeptidomics, Plasmid Preparation, Vaccines, Virus, Concentration Assay, Neutralization, Produced, Inhibition, Activity Assay