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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury
doi: 10.7150/ijbs.85204
Figure Lengend Snippet: Apigenin attenuates Dox-mediated myocardial injury. Mice were treated with Dox (15 mg/kg) with or without oral apigenin co-administration (25 mg/kg) for 4 weeks. (A-C) Serum was isolated from mice and the levels of TnT, BNP, and CK-MB were measured through ELISA. (D) Sirius Red staining was used to observe histological alterations in heart tissue. (E , F) GR-1 immunofluorescence was used to evaluate neutrophil infiltration in the myocardium. (G-H) The transcription of IL-6 and MMP9 in heart tissues was assessed by RT-PCR. *p<0.05.
Article Snippet: The following ELISA kits were used: ATP concentration (LS-F24998, LifeSpan BioSciences), serum Troponin T (TNT) concentration (abx496644, Abbexa),
Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence, Reverse Transcription Polymerase Chain Reaction
Journal: International Journal of Biological Sciences
Article Title: Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury
doi: 10.7150/ijbs.85204
Figure Lengend Snippet: Sirt1 knockdown abolishes the cardioprotective effects of apigenin in mice with Dox-induced cardiomyopathy. Wild-type (WT) and Sirt1 knockout (KO) mice were treated with Dox (15 mg/kg) with or without concomitant oral apigenin (25 mg/kg) administration for 4 weeks. (A-C) Serum levels of TnT, BNP, and CK-MB were measured by ELISA. (D) Sirius Red staining was used to observe histological alterations in heart tissue. (E , F) GR-1 immunofluorescence was used to assess neutrophil infiltration in the myocardium. (G-H) RT-PCR was used to analyze the transcription of IL-6, MCP1, and MMP9 in heart tissues. *p<0.05.
Article Snippet: The following ELISA kits were used: ATP concentration (LS-F24998, LifeSpan BioSciences), serum Troponin T (TNT) concentration (abx496644, Abbexa),
Techniques: Knockdown, Knock-Out, Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence, Reverse Transcription Polymerase Chain Reaction