Journal: Biomimetics

Article Title: Preparation, Characterization, and Drug Delivery of Hexagonal Boron Nitride-Borate Bioactive Glass Biomimetic Scaffolds for Bone Tissue Engineering

doi: 10.3390/biomimetics8010010

Figure Lengend Snippet: ( a , b ) SEM micrographs of the as-received hBN nanopowders; ( c ) digital image of the fabricated glass scaffolds.

Article Snippet: The hexagonal boron nitride (hBN) nanopowders (99.85%+ purity, 65–75 nm, density: 2.3 g/cm 3 ) used in the preparation of the composites were obtained from Nanografi Nanotechnology, Ankara, Turkey.

Techniques:

Journal: Biomimetics

Article Title: Preparation, Characterization, and Drug Delivery of Hexagonal Boron Nitride-Borate Bioactive Glass Biomimetic Scaffolds for Bone Tissue Engineering

doi: 10.3390/biomimetics8010010

Figure Lengend Snippet: ( a ) XRD pattern; ( b ) FTIR spectra of the prepared bioactive scaffolds containing hBN nanopowders.

Article Snippet: The hexagonal boron nitride (hBN) nanopowders (99.85%+ purity, 65–75 nm, density: 2.3 g/cm 3 ) used in the preparation of the composites were obtained from Nanografi Nanotechnology, Ankara, Turkey.

Techniques:

Journal: Biomimetics

Article Title: Preparation, Characterization, and Drug Delivery of Hexagonal Boron Nitride-Borate Bioactive Glass Biomimetic Scaffolds for Bone Tissue Engineering

doi: 10.3390/biomimetics8010010

Figure Lengend Snippet: Optical microscope images of the MC3T3-E1 cells after incubation for 72 h in the presence of composite bioactive glass scaffolds containing ( a ) 0, ( b ) 0.1%, ( c ) 0.2%, ( d ) 0.5%, ( e ) 1%, and ( f ) 2% hBN nanopowders. Magnification at 100×.

Article Snippet: The hexagonal boron nitride (hBN) nanopowders (99.85%+ purity, 65–75 nm, density: 2.3 g/cm 3 ) used in the preparation of the composites were obtained from Nanografi Nanotechnology, Ankara, Turkey.

Techniques: Microscopy, Incubation

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: Expression of IL-6, IL-17, CCR6 and CCL20 in the tongue mucosa of mice. (A) Representative immunohistochemical image of IL-6, IL-17, CCR6 and CCL20 expression in the tongue tissues of mice in the control group, the scratched group, and the scratched + smeared group. Scale bar: 50 μm. (B) Statistical analysis of expression of IL-6, IL-17, CCR6 and CCL20 in the tongue tissues of mice in the control group, the scratched group, and the scratched + smeared group (n = 10/group/day). Asterisks (*) represent significant differences versus the control group; hash symbols (#) represent significant differences between the scratched group and the scratched + smeared group (Student’s t test, * p < 0.05, ** p < 0.01, ## p < 0.01, ns, no significance).

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Expressing, Immunohistochemical staining, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: Typical inflammatory factors were upregulated in mice treated with scratching and E. coli smearing. (A) ELISA was used to detect the expression of IL-6, IL-17, CCR6 and CCL20 in peripheral blood serum samples of mice (n = 10/group/day). Asterisks (*) represent significant differences versus the control group; hash symbols (#) represent significant differences between the scratched group and the scratched + smeared group (Student’s t test, * p < 0.05, ** p < 0.01, ## p < 0.01, ns, no significance). (B) The body weight changes of mice on days 3/5/7 during modeling. The body weight of the control group and the smeared group increased, while the scratched group and the scratched + smeared group decreased. Data present the individual values and mean with SD of each group. Student’s t tests were conducted between the control group and the smeared group, as well as between the scratched group and the scratched + smeared group (n = 10/group/day). * p < 0.05, ** p < 0.01, ns, no significance.

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: RNA signature of EOLP characterized by upregulation of antimicrobial response and inflammatory factors. (A) Volcano plots showed differential expression genes between EOLP-RE group and NEOLP-S group. (B) Count number of IL6 , IL17A , CCR6 , and CCL20 between NEOLP-S (n = 24) and EOLP-RE groups (n = 7) in RNA-seq data. * p < 0.05, ** p < 0.01, **** p < 0.0001. (C) Results of Gene Ontology (GO) enrichment analysis. (D) Results of KEGG pathway analysis. (E) Heat map showed immune infiltration scores between NEOLP-S and EOLP-RE samples based on CIBERSORT. (F-I) Results of Gene Set Enrichment Analysis. (EOLP-RE, erosive oral lichen planus with recurrent erosive oral lichen planus follow-up, NEOLP-S, non-erosive oral lichen planus with stable oral lichen planus follow-up).

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Quantitative Proteomics, RNA Sequencing