Journal: Journal of Functional Foods

Article Title: Membrane vesicles from the probiotic Nissle 1917 and gut resident Escherichia coli strains distinctly modulate human dendritic cells and subsequent T cell responses

doi: 10.1016/j.jff.2019.103495

Figure Lengend Snippet: Fig. 6. Quantification of the Th17 and Treg subsets induced by MV-stimulated DCs. (A-B) Flow cytometric analysis of CD4+ T cells co-incubated for 3 days with untreated mo-DCs (iDC) or mo-DCs primed with MVs of the indicated strains (10 μg/ml) at a DC:T cell ratio of 1:5 with specific markers for: (A) Th17 population (CD4+ CD196+) and (B) Treg (CD25+(High) FoxP3+) population. Representative dot plots of CD4+ cells co-incubated with iDCs (control) or with EcN MV- stimulated DCS are shown for each cell subset analysis. The percentage of positive cells is indicated in each quadrant or box. (C) Data (mean ± SEM) from three- independent experiments performed in triplicate are expressed as fold-change increase in the percentage of positive T cells for Th17 or Treg specific markers induced by MV-stimulated DCs with respect control iDCs induced values (set as 1, dot line). Statistical differences were evaluated by one-way ANOVA followed by Bonferroni’s test (normal distribution) *p < 0.05 versus control DCs.

Article Snippet: For surface marker staining, lymphocytes were first stained with anti-CD4 FITC (clone VIT4), anti-CD25 APC (clone 4E3) and anti-CD196 PE (clone REA190) mAb or with the corresponding isotype-matched conjugated irrelevant antibody (Miltenyi Biotec, Bergish Gladbach, Germany).

Techniques: Incubation, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: Expression of IL-6, IL-17, CCR6 and CCL20 in the tongue mucosa of mice. (A) Representative immunohistochemical image of IL-6, IL-17, CCR6 and CCL20 expression in the tongue tissues of mice in the control group, the scratched group, and the scratched + smeared group. Scale bar: 50 μm. (B) Statistical analysis of expression of IL-6, IL-17, CCR6 and CCL20 in the tongue tissues of mice in the control group, the scratched group, and the scratched + smeared group (n = 10/group/day). Asterisks (*) represent significant differences versus the control group; hash symbols (#) represent significant differences between the scratched group and the scratched + smeared group (Student’s t test, * p < 0.05, ** p < 0.01, ## p < 0.01, ns, no significance).

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Expressing, Immunohistochemical staining, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: Typical inflammatory factors were upregulated in mice treated with scratching and E. coli smearing. (A) ELISA was used to detect the expression of IL-6, IL-17, CCR6 and CCL20 in peripheral blood serum samples of mice (n = 10/group/day). Asterisks (*) represent significant differences versus the control group; hash symbols (#) represent significant differences between the scratched group and the scratched + smeared group (Student’s t test, * p < 0.05, ** p < 0.01, ## p < 0.01, ns, no significance). (B) The body weight changes of mice on days 3/5/7 during modeling. The body weight of the control group and the smeared group increased, while the scratched group and the scratched + smeared group decreased. Data present the individual values and mean with SD of each group. Student’s t tests were conducted between the control group and the smeared group, as well as between the scratched group and the scratched + smeared group (n = 10/group/day). * p < 0.05, ** p < 0.01, ns, no significance.

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Escherichia coli aggravates inflammatory response in mice oral mucositis through regulating Th17/Treg imbalance

doi: 10.3389/fcimb.2025.1585020

Figure Lengend Snippet: RNA signature of EOLP characterized by upregulation of antimicrobial response and inflammatory factors. (A) Volcano plots showed differential expression genes between EOLP-RE group and NEOLP-S group. (B) Count number of IL6 , IL17A , CCR6 , and CCL20 between NEOLP-S (n = 24) and EOLP-RE groups (n = 7) in RNA-seq data. * p < 0.05, ** p < 0.01, **** p < 0.0001. (C) Results of Gene Ontology (GO) enrichment analysis. (D) Results of KEGG pathway analysis. (E) Heat map showed immune infiltration scores between NEOLP-S and EOLP-RE samples based on CIBERSORT. (F-I) Results of Gene Set Enrichment Analysis. (EOLP-RE, erosive oral lichen planus with recurrent erosive oral lichen planus follow-up, NEOLP-S, non-erosive oral lichen planus with stable oral lichen planus follow-up).

Article Snippet: Following cooling and rinsing, a 3% hydrogen peroxide solution was applied to block catalase activity for 10 min, then rinsed and blocked with goat serum for 15 min. After spinning and wiping off the water, 50 μL of IL-6 antibody (1:100, Proteintech, USA), IL-17 antibody (1:1000, Proteintech, USA), CCR6 antibody (1:100, Proteintech, USA), and CCL20 antibody (1:100, Proteintech, USA) were added dropwise on the surface and incubated for 2–3 h at room temperature.

Techniques: Quantitative Proteomics, RNA Sequencing