bms 1 Search Results


u l pd1 pdl1 inhibitor bms 1  (MedChemExpress)
95
MedChemExpress u l pd1 pdl1 inhibitor bms 1
In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle <t>and</t> <t>BMS-1</t> groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)
U L Pd1 Pdl1 Inhibitor Bms 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bms+1/BMS-1/pmc11167788-286-30-38
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a305 109a t  (Bethyl)
92
Bethyl a305 109a t
In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle <t>and</t> <t>BMS-1</t> groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)
A305 109a T, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bms+1/BMS1%2FBMS1L+Antibody/pm38427561-133-29-27
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a305 109a t - by Bioz Stars, 2026-09
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compounds pd  (Selleck Chemicals)
93
Selleck Chemicals compounds pd
In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle <t>and</t> <t>BMS-1</t> groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)
Compounds Pd, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bms+1/BMS-1/ppr0335945-52-0-12
Average 93 stars, based on 1 article reviews
compounds pd - by Bioz Stars, 2026-09
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anti bms1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti bms1
In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle <t>and</t> <t>BMS-1</t> groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)
Anti Bms1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti bms1 - by Bioz Stars, 2026-09
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mtz  (Addgene inc)
91
Addgene inc mtz
In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle <t>and</t> <t>BMS-1</t> groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)
Mtz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bms+1/pDONR221-Bms1+(Plasmid+%23100001)/pm35132245-223-34-228
Average 91 stars, based on 1 article reviews
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pd-l1 inhibitor (bms-1  (Xi'an Tianlong Science)
90
Xi'an Tianlong Science pd-l1 inhibitor (bms-1
Schematic illustration of Pt NPs conjugated with <t>BMS-1</t> through hyperthermia-sensitive linkage for NIR-controlled release of inhibitor and exposure of Mal . A thermal-sensitive release and Mal deprotection procedure is achieved by the Retro D-A reaction. Exposed Mal on the surface of Pt NPs captures the antigens from ablated tumor cells and promotes antigen presentation. The released BMS-1 alleviates T cell exhaustion and induces infiltration of effector T cells.
Pd L1 Inhibitor (Bms 1, supplied by Xi'an Tianlong Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bms1 domain protein  (Ribobio co)
90
Ribobio co bms1 domain protein
Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
Bms1 Domain Protein, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bms 1  (Nacalai)
86
Nacalai bms 1
Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
Bms 1, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bms 1 - by Bioz Stars, 2026-09
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chemically crosslinked hydrogel with dox and bms-1 system pf-127-cho/chi dox and bms-1  (CH Instruments)
90
CH Instruments chemically crosslinked hydrogel with dox and bms-1 system pf-127-cho/chi dox and bms-1
Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
Chemically Crosslinked Hydrogel With Dox And Bms 1 System Pf 127 Cho/Chi Dox And Bms 1, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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28-gauge tail vein catheter mtv-bms1  (Strategic Applications Inc)
90
Strategic Applications Inc 28-gauge tail vein catheter mtv-bms1
Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
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bms 1  (Bristol Myers)
86
Bristol Myers bms 1
Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.
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bms1l bms1 nm 014753 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles BMS1 mGFP tagged Human BMS1 homolog ribosome assembly protein yeast BMS1 200ul 10 7 TU mL
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Image Search Results


In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle and BMS-1 groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)

Journal: Respiratory Research

Article Title: TMPRSS2 is a tumor suppressor and its downregulation promotes antitumor immunity and immunotherapy response in lung adenocarcinoma

doi: 10.1186/s12931-024-02870-7

Figure Lengend Snippet: In vivo and in vitro experimental validation of the bioinformatics findings. TMPRSS2-knockdown tumors display increased tumor-infiltrating lymphocytes, expression of immune checkpoint molecules, and sensitization to immune checkpoint inhibitors. a TMPRSS2 knockdown markedly promoted proliferative and invasive abilities of A549 cells. b TMPRSS2 knockdown increased tumor volume and progression in Lewis tumor mouse models. Lewis tumor cells transfected with ShCon or ShTMPRSS2 lentivirus were subcutaneously injected into mice. The tumor volumes were measured every three days from the fifth day to the fifteenth. Data represent mean ± SEM. SEM, standard error of mean. ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001. c TMPRSS2 knockdown increased MSH6 expression in A549 cells, as evidenced by Western blotting. d TMPRSS2 knockdown enhanced the expression of MHC class I genes ( HLA-A , HLA-B , and HLA-C ) in A549 cells, as evidenced by real-time qPCR. e NK cells co-cultured with TMPRSS2 -knockdown A549 cells showing higher proliferation capacity than NK cells co-cultured with TMPRSS2 -wildtype A549 cells, as evidenced by the EDU proliferation assay. f CD8, CD49b, and PD-L1 immunofluorescence staining in Lewis orthotopic tumors and H-score analysis. ShTMPRSS2 versus shCon group, n = 6 for each group, two-tailed Student’s t test, *** p < 0.001. g - j Comparisons of TNF-α, IFN-γ, PD-1, and LAG3 expression on CD8 + T cells from tumor-infiltrating lymphocytes (TILs) in tumor-bearing mice between TMPRSS2 -knockdown and TMPRSS2 -wildtype group (ShTMPRSS2 versus ShCon group, n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001). TILs were stained with CD3, CD8, TNF-α, and IFN-γ and were then analyzed by flow cytometry. Lymphocytes were gated according to forward scatter and side scatter. CD3 and CD8 staining was used to identify CD8 + T cells. k - m TMPRSS2 -knockdown tumors formed by subcutaneous injection of Lewis cells, as mentioned in ( b ). shCon and shTMPRSS2 tumor-bearing mice were divided into vehicle and BMS-1 groups. The vehicle and BMS-1 groups of mice were treated with solvent and BMS-1, respectively. k Representative images of tumor-bearing mice shown on the left. The right graph showing the change of tumor size in the tumor-bearing mice over time. Data represent mean ± SEM ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001); Comparison of the volume ratios of mice tumors after and before treatment with BMS-1 between TMPRSS2 -knockdown and TMPRSS2 -wildtype groups (two-tailed Student’s t test, *** p < 0.001). Comparisons of TNF-α ( l ) and IFN-γ ( m ) expression on CD8 + T cells from TILs in tumor-bearing mice ( n = 6 for each group, two-tailed Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: After 5 days, when the tumor volume was approximately 4–5 mm3, the mice were randomly divided into six groups, with half of the ShCon and ShTMPRSS2 mice treated with 150 U/L PD1/PDL1 inhibitor BMS-1 (concentration 500 mg/mL; i.p.) (MCE Cat. No. HY-19991) every 3 days.

Techniques: In Vivo, In Vitro, Biomarker Discovery, Knockdown, Expressing, Transfection, Injection, Two Tailed Test, Western Blot, Cell Culture, Proliferation Assay, Immunofluorescence, Staining, Flow Cytometry, Solvent, Comparison

Schematic illustration of Pt NPs conjugated with BMS-1 through hyperthermia-sensitive linkage for NIR-controlled release of inhibitor and exposure of Mal . A thermal-sensitive release and Mal deprotection procedure is achieved by the Retro D-A reaction. Exposed Mal on the surface of Pt NPs captures the antigens from ablated tumor cells and promotes antigen presentation. The released BMS-1 alleviates T cell exhaustion and induces infiltration of effector T cells.

Journal: Bioactive Materials

Article Title: Synergistic enhancement of immunological responses triggered by hyperthermia sensitive Pt NPs via NIR laser to inhibit cancer relapse and metastasis

doi: 10.1016/j.bioactmat.2021.05.030

Figure Lengend Snippet: Schematic illustration of Pt NPs conjugated with BMS-1 through hyperthermia-sensitive linkage for NIR-controlled release of inhibitor and exposure of Mal . A thermal-sensitive release and Mal deprotection procedure is achieved by the Retro D-A reaction. Exposed Mal on the surface of Pt NPs captures the antigens from ablated tumor cells and promotes antigen presentation. The released BMS-1 alleviates T cell exhaustion and induces infiltration of effector T cells.

Article Snippet: Maleimide-PEG 2k -COOH (Mal-PEG 2k -COOH) and PD-L1 inhibitor (BMS-1) were purchased from Xi'an ruixi Biological Technology Co., Ltd, China. mPEG 2k -OH was purchased from Sigma-Aldrich.

Techniques:

Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.

Journal: PLoS Genetics

Article Title: The RNA Helicases AtMTR4 and HEN2 Target Specific Subsets of Nuclear Transcripts for Degradation by the Nuclear Exosome in Arabidopsis thaliana

doi: 10.1371/journal.pgen.1004564

Figure Lengend Snippet: Proteins co-purified with MTR4-GFP and identified by LC-MS/MS.

Article Snippet: AT1G06720 , BMS1 domain protein , Ribo. bio. , 269.4 , 5 , 319.4 , 4 , 430.2 , 10.

Techniques: Binding Assay