Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 1. Specificity of anti-BMPRIA, anti-BMPRIB and anti-BMPRII antibodies. (A) Wells containing anti-BMPRIA antibody (IA-ab) show intense ELISA reaction only when coated by BMPRIA protein (IA-ag), but not BMPRIB protein (IB-ag). Wells containing anti-BMPRIB antibody (IB-ab) show intense ELISA reaction only when coated by BMPRIB protein (IB-ag), but not BMPRIA protein (IA-ag). Wells containing anti-BMPRII antibody show intense ELISA reaction only when coated by BMPRII protein (). (B) anti-BMPRIA, anti-BMPRIB and anti-BMPRII antibodies specifically recognize the corresponding proteins in a dose-dependent manner. (C–H) Photomicrographs of pre-absorption tests; control (C–E), pre-absorbed (F–H). Note that immunoreactivities in the layer V of the cortex are completely abolished by pre-absorption tests. Scale bar100 m for (C–H).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 2. BMPRIA, BMPRIB and BMPRII expressions in the rat whole brain. 12, hypoglossal nucleus; Amy, amygdala; AP, area postrema; cc, corpus callosum; Cer, cerebellum; CN, cerebellar nuclei; Cor, cerebral cortex; CPu, caudate putamen; DH, dorsal horn; Hi, hippocampus; IO, inferior olive; Pir, piriform cortex; VC, ventral cochlear nucleus, VH, ventral horn. Scale bar3 mm for (A–I); 2 mm for (J–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 3. BMPRIA, BMPRIB and BMPRII expressions in the olfactory bulb (A–I) and medial septal nucleus (J–L). EPl, external plexiform layer; Gl, glomerular layer; Gr, granular layer; MS, medial septal nucleus; ON, olfactory nerve layer; SE, subependymal layer. Scale bar 200 m for (A, D, G); 100 m for (B, C, E, F, H–L).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 4. BMPRIA, BMPRIB and BMPRII expressions in the cerebral cortex (A–D, F–I, K–N) and piriform cortex (E, J, O). I–VI, layers I–VI; Pir, piriform cortex. Scale bar200 m for (A, E, F, J, K, O); 100 m for (B–D, G–I, L–N).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 5. BMPRIA, BMPRIB and BMPRII expressions in the hippocampus. CA1-3, fields CA1-3 of Ammon’s horn; DG, dentate gyrus; Gr, granular layer; LM, stratum lacnosum-moleculare; Mo, stratum moleculare; Or, stratum oriens; Po, polymorphological layer; Py, stratum pyramidale; Ra, stratum radiatum. Scale bar 500 m for (A, D, G); 100 m for (B, E, H, J–L); 50 m for (C, F, I, M–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 6. BMPRIA, BMPRIB and BMPRII expressions in the basolateral amygdaloid nucleus (A–C), caudate putamen (D–F), globus pallidus (G–I) and corpus callosum (J–O). BL, basolateral amygdaloid nucleus; cc, corpus callosum; CPu, caudate putamen; dhc, dorsal hippocampal commissure; GP, globus pallidus. Scale bar100 m for (J–L); 50 m for (A–I, M–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 7. BMPRIA, BMPRIB and BMPRII expressions in the mediodorsal thalamic nucleus (A–C), reticular thalamic nucleus (D–F), supraoptic nucleus (G–I), red nucleus (J–L), substantia nigra, reticular part (M–O) and interpeduncular nucleus (P–R). IP, interpeduncular nucleus; MD, mediodorsal thalamic nucleus; RN, red nucleus; Rt, reticular thalamic nucleus; SNR, substantia nigra, reticular part; SO, supraoptic nucleus. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 8. BMPRIA, BMPRIB and BMPRII expressions in the fascial nerve nucleus (A–C), facial nerve (D–F), hypoglossal nucleus (G–I), ambiguus nucleus (J–L), nucleus of the solitary tract (M–O) and mesencephalic trigeminal nucleus (P–R). 7, facial nucleus; 7n, facial nerve; 12, hypoglossal nucleus; Amb, ambiguus nucleus; Me5, mesencephalic trigeminal nucleus; SOL, nucleus of the solitary tract. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 9. BMPRIA, BMPRIB and BMPRII expressions in the dorsal cochlear nucleus (A, C, E), ventral cochlear nucleus (B, D, F), lateral vestibular nucleus (G–I), vestibulo-cochlear nerve (J–L), inferior colliculus (M–O), olivary pretectal nucleus (P–R) and superior colliculus (S–U). 8n, vestibulo- cochlear nerve; DC, dorsal cochlear nucleus; IC, inferior colliculus; LVe, lateral vestibular nucleus; opt, optic tract nucleus; SC, superior colliculus; VC, ventral cochlear nucleus. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 10. BMPRIA, BMPRIB and BMPRII expressions in the locus ceruleus (A–C), pyramidal tract (D–F), inferior olive (G, J, M), cerebellar cortex (H, K, N), and cerebellar nuclei (I, L, O). CN, cerebellar nuclei; Gr, granular layer; IO, inferior olive; LC, locus ceruleus; Mol, molecular layer; Pur, Purkinje cell layer; Py, pyramidal tract. Scale bar50 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 11. BMPRIA, BMPRIB and BMPRII expressions in the spinal cord (A–I), subventircular zone (J–L), choroidal plexus (M–O). Ch, choroidal plexus; DH, dorsal horn; VH, ventral horn; SVZ, subventricular zone; wm, white matter. Scale bar500 m for (A–C); 50 m for (D–O).

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques:

Journal: Neuroscience

Article Title: Bone morphogenetic protein receptor expressions in the adult rat brain.

doi: 10.1016/j.neuroscience.2010.12.027

Figure Lengend Snippet: Fig. 12. Double-staining study showing that BMPRIA, BMPRIB and BMPRII positive cells in the corpus callosum (A, D, G) are also positive for GFAP (B, E, H) in a merged photomicrograph (C, F, I). Scale bar20 m.

Article Snippet: Solutions of 4 g/ml of recombinant human BMPRIA (315-BR100; R & D Systems, Inc., Mineapolis, MN, USA; http://www. rndsystems.com), BMPRIB (505-PR-100; R & D Systems, Inc.) and BMPRII (H00000659-Q01; Abnova Coporation Inc., Taiwan; http:// www.abnova.com) in 0.1 M carbonate buffer (pH 9.6) were prepared, 50 l of each antigen solution was added to the corresponding wells (96 Well EIA/RIA Plate; Corning, Inc., Corning, NY, USA), and then incubated for overnight at 4 °C.

Techniques: Double Staining

Journal: Biology

Article Title: BMP-2-Driven Osteogenesis: A Comparative Analysis of Porcine BMSCs and ASCs and the Role of TGF-β and FGF Signaling.

doi: 10.3390/biology14060610

Figure Lengend Snippet: Figure 6. Receptor expressions of ALK2, ALK4, ALK6, ALK5, ALK7, BMPR-II in pASC. Grouped representation of the respective receptor expression in the course of osteogenic differentiation (OM +/−BMP-2). From day 19, there is a significant induction of ALK 2, ALK 6, and ALK 5 with the addition of BMP-2. BMPR-II expression in the OM group decreased in OM and tended to stay increased under BMP-2 supplementation from day 19, but was not considered significant (* p ≤0.05, ** p ≤0.01; n = 6, BMP-2 450 ng/mL).

Article Snippet: The respective conjugated antibodies were used for the expressions of ALK3 (Cat. No.: AF436), ALK 5 (Cat. No.: FAB5871), ALK6 (Cat. No.: FAB5051A), TGF-β2-RII (Cat. No.: FAB532P), ALK7 (Cat. No.: FAB77491A), ALK2 (Cat. No.: AF637), ALK4 (Cat. No.: MAB2221), and BMPR-II (Cat. No.: AF811) (by R&D Systems, Minneapolis, MN, USA), and the pASCs and pBMSCs were compared for their expressions of the specific surface antigens CD45 (Cat. No.: MCA1568GA, BioRad, Hercules, CA, USA), HLA-DR (human leukocyte antigen–antigen D-related surface molecule) (Cat. No.: MCA2314F, Bio-Rad, Hercules, CA, USA), CD29 (Cat. No.: 561,496, BD Pharmingen, Franklin Lakes, NJ, USA), CD79alpha (Bio-Rad, Cat. No.: MCA2538GA), CD14 (Cat. No.: MCA1568GA, Bio-Rad, Hercules, CA, USA), CD31 (Cat. No.: AF3387, R&D Systems, Minneapolis, MN, USA), CD105 (Cat. No.: NB110-58718APC, Novus Biologicals, Minneapolis, MN, USA), CD26 (, Cat. No.: NB600-552APC, Novus Biologicals, Minneapolis, MN, USA), CD73 (, Cat. No.: AF4488, R&D Systems, Minneapolis, MN, USA), CD90 (Cat. No.: 559,869, BD Pharmingen, Franklin Lakes, NJ, USA), CD34 (Cat. No.: 81289, abcam, Cambridge, UK), and CD44 (Cat. No.: 5531, BD Pharmingen, Franklin Lakes, NJ, USA).

Techniques: Expressing

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Characterization of BmprIB+ dermal cells. (A): Immunohistochemical staining of BmprIB in human foreskin. (B): Percentage evaluation of BmprIB expression in freshly isolated human dermal cells by flow cytometry. Histograms of BmprIB expression (left; 3.5% ± 0.4%) and isotype control (right). (C): Cell morphology under phase‐contrast microscopy. (D): The proliferative potential was assessed with the Alamar Blue assay in usDCs and BmprIB+ cells. Cell numbers were quantified by the absorbance wavelength at 570 nm using a spectrophotometer at 24, 48, 72, and 96 hours. Data represented the mean of three individuals and each of them was the mean of triplicate experiments. The osteogenic potential of BmprIB+ cells was demonstrated by ALP staining (E) and ARS staining (F) . (G): The mRNA expression levels of ALP (at day 7) and OCN , OPN , and BSP (at day 21) were analyzed by real‐time polymerase chain reaction after osteogenic induction. Data represent the mean ± SD ( n = 3). ∗, p < .05 indicates statistical significance. Scale bars = 50 µm. Abbreviations: ALP, alkaline phosphatase; ARS, alizarin red S stain; BmprIB, bone morphogenetic protein receptor type IB; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Immunohistochemical staining, Staining, Expressing, Isolation, Flow Cytometry, Control, Microscopy, Alamar Blue Assay, Spectrophotometry, Real-time Polymerase Chain Reaction

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Dermal cell/coral scaffold compatibility. (A): Gross view of a coral scaffold with the size of 4‐mm diameter by 1‐mm thickness (top panel); two‐dimensional (left) and three‐dimensional images (right) of a coral scaffold by µCT scanning (bottom panel). (B): The cell proliferation analysis of the BmprIB+ cells and usDCs on the coral scaffold was assessed with the Alamar Blue assay at 12, 24, 36, 48, 60, 72, 84, and 96 hours. Data represent the mean of three individuals and each of them was the mean of triplicate experiments. (C): Scanning electron microscopy evaluation of the adherence and matrix deposition of BmprIB+ cells on the coral scaffold 1, 3, and 7 days after seeding. Scale bar = 20 µm. The coral alone without cell seeding served as a control. Scale bar = 100 µm. (D): Osteogenic potential of BmprIB+ cells and usDCs on the coral scaffold was evaluated according to the ALP activity and osteocalcin content at days 1, 3, 7, 14, 21, and 28 after osteogenic induction, respectively. Data represent the mean of three individuals and each of them was the mean of triplicate experiments. Abbreviations: 2D, two‐dimensional; ALP, alkaline phosphatase; BmprIB, bone morphogenetic protein receptor type IB; μCT, microcomputed tomography; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Alamar Blue Assay, Electron Microscopy, Control, Activity Assay, Tomography

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Evaluation of early‐stage osteogenic reconstruction. (A): A full‐thickness defect 4 mm in diameter was created by a hollow drill on the right side of the calvarial bone and repaired by coral alone, usDCs/coral, or BmprIB+ cells per coral in each group (top panel). Representative images of two‐dimensional (left) and three dimensional (right) µCT scanning were taken 24 hours after the surgery (bottom panel). (B): Histological analyses including H&E staining and Masson trichrome staining were used to evaluate new bone formation 6 weeks postimplantation. The black arrows indicate both ends of the defect. Scale bar = 1 mm. (C): Immunostaining was used to evaluate osteogenic marker expression, including osteocalcin, osteopontin, bone sialoprotein, and vascular marker CD31 in newly formed bone. Scale bar = 50 µm. (D): Histomorphometric analysis was used to quantify the percentage of positive staining area or vascular count. Data represent the average ± SD ( n = 6). ∗, p < .05 indicates statistical significance. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; H&E, hematoxylin and eosin; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Staining, Immunostaining, Marker, Expressing

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Microcomputed tomography (µCT) evaluation of late‐stage osteogenic reconstruction. (A): The defect was repaired by engineered bone on the right side of the mouse cranium 24 weeks postimplantation. Black arrows mark the repaired defect. (B): Representative three‐dimensional µCT images of parietal bones from each group 24 weeks postimplantation. Scale bar = 5 mm (left) and 1 mm (right). (C): The opaque percentage of each group was evaluated. (D): Other important bone structure parameters, such as BV (mm 3 ), BV/TV ratio (%), the Tb.N (1/mm), the Tb.Th (mm), the Tb.Sp, and Conn.D, were compared among these three implant groups with coral, usDCs/coral, and BmprIB/coral. Data represent the mean ± SD ( n = 6). ∗, p < .05 indicates statistical significance. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; BV, bone volume; BV/TV, ratio of bone volume to total volume; Conn.D, connectivity density; Tb.N, number of trabeculae; Tb.Sp, trabecular spacing; Tb.Th, thickness of the trabecular structure; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Tomography

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Histological evaluation of late‐stage osteogenic reconstruction. (A): Goldner trichrome staining was used for plastic‐embedded, undecalcified, 24‐week regenerated bone. Mature bone matrix stained green and immature new bone matrix stained red. (B): Hematoxylin and eosin staining was also used to evaluate 24‐week regenerated bone. The black arrows indicate both ends of the defect. Scale bar = 1 mm. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Staining