Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Physiological Regulation of Bmp8b (A) Tissue distribution of Bmp8a and Bmp8b mRNA, relative to levels in testes. n = 8 mice, age 12 weeks. (B) BMP expression in stromal-vascular (SV) and mature adipocyte (MA) fractions of BAT. n = 6, age 12 weeks. (C) mRNA expression of BMPs throughout differentiation of brown adipocytes in vitro. n = 3 experiments, each in triplicate, relative to day 6 levels. (D) BAT expression of BMPs in mice fed HFD for 12 weeks or housed at 4°C for 3 weeks (CE), relative to chow fed housed at 23°C (CHOW). n = 7, age 16 weeks. (E) BAT expression of BMPs in fed, fasted, and refed animals. (F) BAT expression of BMP8B in 12-week-old mice after 7 days of treatment with vehicle or specific β3 agonist CL 316243 (1 mg/kg/24 hr via subcut osmotic pump). (G) BAT BMP8B expression in mice given control (vehicle = 10 mM NaOH), mild (5 μg T4 + 2 μg T3/100 g/day), or intensive (20 μg T4 + 8 μg T3/100 g/day) hyperthyroid treatment. n = 3 per group, age 12 weeks. (H) Bmp8b expression in differentiated brown adipocytes treated with vehicle or T3 (5 nM) for 6 hr. n = 2 experiments, each in triplicate. (I) Luciferase activity in HIB-1B cells driven by Bmp8b promoter in response to cotransfection with PPARα or γ and specific chemical agonists Rosiglitazone or GW7647 and dibutyril-cAMP. n = 4 experiments, each in triplicate. (J) Bmp8b expression in mice treated with vehicle or 25 mg/kg of PPARα agonist WY14643 via five daily IP injections. (K) Bmp8b mRNA expression in wild-type and PPAR −/− mice at 30°C and 4°C. n = 3 per group. Error bars indicate SEM. See also Figure S1 .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Expressing, In Vitro, Control, Luciferase, Activity Assay, Cotransfection

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Regulation of the BMP8B Promoter, Related to Figure 1 (A) Luciferase activity in HEK293 cells driven from 3 kb of the BMP8B promoter following co-transfection with plasmids overexpressing the indicated TR isoforms. n = 3 experiments carried out in triplicate. (B) Schematic diagram of 3 kb of the murine BMP8B promoter indicating location and sequences of the 2 identified candidate PPREs. ∗ p < 0.05 using ANOVA. Error bars indicate SEM.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Luciferase, Activity Assay, Cotransfection

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Obesogenic Phenotype of Bmp8b −/− Mice (A and B) (A) Rate of energy expenditure at 4 weeks of age by using ANCOVA (adjusted body weight = 16.98 g) and (B) food intake in the same mice. n = 6–8. (C and D) (C) Growth curves of wild-type (WT) and Bmp8b −/− (KO) animals fed chow or HFD postweaning with (D) energy expenditure by using ANCOVA (adjusted body weight = 23.82 g). (E–H) (E) Daily food intake and (F) fat mass at 95 days in the same animals, followed by (G) body weights and (H) fat mass at sacrifice (185 days). n = 7. (I) Body temperature measured via subcutaneous chip over 7 days. (J) NE stimulated oxygen consumption in WT and KO mice housed at thermoneutrality (30°C) or cold exposure (4°C) for 3 weeks, expressed as VO 2 over time and (K) ΔVO2 from baseline. n = 7. Annotation indicates significant effect of a = genotype, b = diet, or c = significant diet-genotype interaction. p < 0.05 using ANOVA or ANCOVA. AUC, area under curve. Error bars indicate SEM. See also Figure S2 and .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques:

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Evaluation of Metabolism in Bmp8b −/− Mice, Related to Figure 2 (A) Locomotor activity measured by beam breaks over 48 hr in mice depicted in Figure 2 . (B) Serum estradiol levels in serum of chow fed animals aged 12 weeks. n = 4. (C) Bone mineral density measured by DEXA at sacrifice. n = 7. (D) Glucose tolerance test and insulin tolerance test performed in WT and KO mice fed chow and high-fat diet. Mice were given a bolus of glucose (1 g/Kg i.p) or insulin (0.75 IU/Kg i.p) corrected for body weight and subsequent measurements of blood glucose were taken at the indicated time points following injection. Error bars indicate SEM. Annotation indicates significant effect of a = genotype, b = diet or c = significant diet-genotype interaction. p < 0.05 using ANOVA, AUC = area under curve.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Activity Assay, Injection

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Characterization of Bmp8b −/− BAT (A) Haematoxylin and eosin sections from interscapular BAT of WT and KO animals fed chow or HFD for 4 months postweaning. (B and C) (B) Lipid droplet size and (C) number of nuclei per field, scored visually by using arbitrary units. n = 2 sections per mouse, 7 per group. (D and E) (D) Weights of the same BAT depots and (E) expression of genes associated with thermogenesis, adipogenesis, and lipid handling in BAT. n = 7 animals per group, age 5 months. (F–J) Quantification of indicated phosphoproteins in the Smad and P38 MAPK pathways in BAT of WT and KO mice with (J) representative images of blots. n = 5–8 per group. (K) Direct measurement of SNA to BAT of WT and KO mice in response to controlled lowering of body temperature for 30 min. n = 7 per group, age 12 weeks. (L) Expression of genes encoding adrenergic receptors and associated signaling proteins in BAT of WT and KO mice. n = 7 per group, age 16 weeks. Error bars indicate SEM. Annotation indicates significant effect of a = genotype, b = diet, or c = significant diet-genotype interaction, defined as p < 0.05 using two-way ANOVA. ∗ p < 0.05 using ANOVA.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Expressing

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: BMP8B-Mediated Alterations to Brown Adipocyte Lipolysis (A–C) (A) Levels of phospho-Smad 1/5/8 in differentiated brown adipocytes following 2 hr treatment with 100 pM BMP8B, (B) pHSL, and (C) pAMPK after 1 and 2 hr treatment. (D and E) (D) Phospho-P38 MAPK and (E) phospho-CREB levels after subsequent 10 min stimulation with NE. Blots represent three experiments performed in duplicate/triplicate with phospho levels normalized to the total protein. (F) Lipolytic activity in differentiated brown adipocytes stimulated with NE (75 nM) after treatment with vehicle (control), 100 pM BMP8B for 2 hr (acute BMP8B), or throughout differentiation (chronic BMP8B). n = 3 experiments, in triplicate. (G and H) (G) Lipolytic dose-response curves for cells pretreated for 2 hr with 100 pM BMP8B followed by 2 hr of NE and (H) NE stimulated dose-response in absence or presence of 10 μM α2AR antagonist RS79948. (I) Effect of BMP8B on isoprenaline dose response. (J) Effect of 10 μM ALK7 antagonist SB431542 on NE dose response following BMP8B treatment. (K) Effect of ALK7 inhibition on rate of NE-stimulated lipolysis in BMP8B-treated brown adipocytes. n = 8. Error bars indicate SEM. ∗ p < 0.05 using ANOVA. AUC, area under curve. See also Figure S3 .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Activity Assay, Control, Inhibition

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: BAT Expression Profile of Candidate BMP Receptors, Related to Figure 4 (A) mRNA expression levels of potential BMP8B receptors in fractionated BAT and WAT from wild-type mice, n = 8 aged 12 weeks and (B) the same genes measured in an immortalized BAT cell line both pre- and post-differentiation. N = 3 experiments in duplicate. ∗ p < 0.05 using ANOVA. Error bars indicate SEM.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Expressing

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Characterization of Bmp8b −/− Brains (A) Expression of Bmp8b mRNA in brain regions dissected from fed mice age 12 weeks. n = 6. (B) Bmp8b mRNA in the ventromedial and arcuate nuclei of the hypothalamus. n = 8 mice, age 12 weeks. (C) In situ hybridization showing key neuropeptide mRNA regulating feeding (AgRP, NPY, POMC, and CART) in arcuate nucleus of the hypothalamus in WT and Bmp8b −/− mice. n = 7 per group, age 5 months, that was fed chow, accompanied by representative images and densitometric analysis. (D–F) (D) Western blot analysis of the fatty acid synthesis pathway in hypothalamus of WT and Bmp8b −/− mice following 12 weeks of chow or HFD treatment, showing levels of activated AMPKα and pACCα alongside (E) total levels of FAS, ACCα, AMPKα1, and AMPKα2, which are all normalized to β-actin with (F) representative immunoblots. n = 6–8. Error bars indicate SEM. c = significant diet-genotype interaction. p < 0.05 using ANOVA or two-way ANOVA, depending on the number of variables. See also Figure S4 .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Expressing, In Situ Hybridization, Western Blot

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Effects of Cold Exposure on the Hypothalamic Fatty Acid Synthesis Pathway, Related to Figure 5 Western blot analysis of the fatty acid synthesis pathway in hypothalamus of wild-type and Bmp8b −/− mice following 3 weeks housing at 22°C or 4°C to induce thermogenesis in BAT, showing levels of (A) activated AMPK and activated ACCα alongside (B) levels of total FAS, ACCα, AMPKα1 and AMPKα2, normalized to β-actin with (C) representative blots, n = 8. ∗ p < 0.05 using ANOVA. Error bars indicate SEM.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Western Blot

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Thermogenic Effect of Central BMP8B Treatment (A) Core body temperature measured by rectal probe following ICV injection with 2 μl of 100 pM BMP8B or vehicle. n = 6–8. (B) Change to body weight of the same animals 4 hr postinjection. n = 6–8. (C) Expression of thermogenic genes in BAT. n = 6–8. (D) One hour post-ICV analysis of pAMPKα and pACCα in the hypothalamus of mice treated with BMP8B or vehicle with representative blots, normalized to β-actin. n = 6–8. (E and F) (E) Numbers of c-FOS immunoreactive (IR) neurons in RPa and inferior olive (IO) under the same conditions with (F) representative sections (Gi, gigantocellular reticular nucleus; IO, inferior olive; py, pyramidal tract; RPa, raphe pallidus; scale bar, 200 μm). n = 5. (G) Change in SNA to BAT and kidney of mice following ICV vehicle or BMP8B, injection at time 0. n = 4–6, age 12–14 weeks. (H–L) (H) Daily body weights of mice treated with chronic BMP8B (100 pM) or vehicle via ICV cannular connected to subcut pump with corresponding food intake and circulating (I) insulin, (J) glucose, (K) triglycerides, and (L) free fatty acids at end of stud. n = 7, age 12 weeks. Error bars indicate SEM. ∗ p < 0.05 using ANOVA. AUC, area under curve. See also Figure S5 .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Injection, Expressing

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: Response to Chronic BMP8B ICV Infusion and Justification of Dose, Related to Figure 6 (A) Body weight and (B) food intake following chronic infusion with vehicle or 100 nM rhBMP8B (0.25 μl/hr) for 2 weeks, via subcutaneous osmotic pumps. Arrow indicates implantation of pumps, n = 7 per group. (C) Dose response in mature brown adipocytes stimulated with 75 nM NE after treatment with differing concentrations of BMP8B using lipolysis as a readout via free glycerol release. n = 3 experiments carried out in triplicate, ∗ p < 0.05 using ANOVA. Error bars indicate SEM.

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques:

Journal: Cell

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

doi: 10.1016/j.cell.2012.02.066

Figure Lengend Snippet: AMPK in the VMH Regulates the Thermogenic Effect of BMP8B (A and B) (A) Representative c-FOS images and (B) total number of c-FOS immunoreactive (IR) neurons in LHA and VMH of mice treated for 2 hr with 2 μl ICV vehicle, 100 pM BMP8B, or BMP8B + 10 uM SB431452. n = 8, age 12 weeks (3V = third ventricle, f = fornix). (C and D) (C) Core body temperature after 2 hr ICV BMP8B treatment in female rats expressing either GFP, DN, or constitutively active (CA) AMPKα in the VMH with (D) mRNA expression in BAT of the same rats, explanted after final treatment. (E–G) (E) Representative thermal images of rats corresponding to the different AMPKα isoforms 2 hr postvehicle or post-BMP8B treatment with (F) spot temperatures adjacent to interscapular BAT depot (dotted line) and (G) correlation between core temperature and BAT temperature increase. n = 8–16. Error bars indicate SEM. Female Sprague-Dawley rats/group, age 10 weeks, mean of four separate 2 hr BMP8B treatments. ∗ p < 0.05, ∗∗ p < 0.005, using ANOVA. See also Figure S6 .

Article Snippet: Recombinant human BMP8B was purchased from R&D Systems.

Techniques: Expressing

Journal: Frontiers in Pediatrics

Article Title: Bone Morphogenetic Protein-8B Levels at Birth and in the First Year of Life: Relation to Metabolic-Endocrine Variables and Brown Adipose Tissue Activity

doi: 10.3389/fped.2022.869581

Figure Lengend Snippet: Circulating BMP8B concentrations in human infants at birth and at age 4 and 12 months, in mothers of those infants during the third trimester of pregnancy (gestational age: 35.3 ± 0.39 weeks) and in healthy adult women and men. Data are shown for the two sexes pooled (A) and split by sex (B) . Gray dots represent girls, pregnant women and healthy adult women, and white dots correspond to boys and to healthy adult men. *** P < 0.001 vs. pregnant women; + P < 0.05 and +++ P < 0.001 vs. healthy adults; ### P < 0.001 vs. 12 months; δ P = 0.04 vs. boys.

Article Snippet: For qRT-PCR, the BMP8B TaqMan Gene Expression assay probe Hs01629120 was used, with reaction mixtures containing 1 μL cDNA, 10 μL TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, United States), 250 nM probes and 900 nM of primers from the Assays-on-Demand Gene Expression Assay Mix (Thermo Fisher Scientific, Waltham, MA, United States).

Techniques:

Journal: Frontiers in Pediatrics

Article Title: Bone Morphogenetic Protein-8B Levels at Birth and in the First Year of Life: Relation to Metabolic-Endocrine Variables and Brown Adipose Tissue Activity

doi: 10.3389/fped.2022.869581

Figure Lengend Snippet: Longitudinal results of circulating BMP8B concentrations in infant girls (A) and boys (B) . The dotted line depicts the mean values in apparently healthy adults ( N = 26; 15 women and 11 men); the shaded area represents the standard error (SEM) in those healthy adults. # P < 0.05 vs. 4 months; * P < 0.05 and *** P < 0.001 vs. 12 months; + P < 0.05 and +++ P < 0.001 vs. healthy adults.

Article Snippet: For qRT-PCR, the BMP8B TaqMan Gene Expression assay probe Hs01629120 was used, with reaction mixtures containing 1 μL cDNA, 10 μL TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, United States), 250 nM probes and 900 nM of primers from the Assays-on-Demand Gene Expression Assay Mix (Thermo Fisher Scientific, Waltham, MA, United States).

Techniques:

Journal: Frontiers in Pediatrics

Article Title: Bone Morphogenetic Protein-8B Levels at Birth and in the First Year of Life: Relation to Metabolic-Endocrine Variables and Brown Adipose Tissue Activity

doi: 10.3389/fped.2022.869581

Figure Lengend Snippet: BMP8B expression levels in brown adipose tissue (BAT, N = 5), white adipose tissue (WAT, N = 5) and liver ( N = 3) from newborns, and in placenta ( N = 4) and cord blood cells ( N = 4). Data are mean ± SEM of relative levels of BMP8B mRNA (BMP8B mRNA/18S rRNA). * P = 0.031 vs. cord blood cells; + P = 0.035 vs. liver; # P = 0.007 vs. WAT.

Article Snippet: For qRT-PCR, the BMP8B TaqMan Gene Expression assay probe Hs01629120 was used, with reaction mixtures containing 1 μL cDNA, 10 μL TaqMan Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, United States), 250 nM probes and 900 nM of primers from the Assays-on-Demand Gene Expression Assay Mix (Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Expressing

Journal: Frontiers in Endocrinology

Article Title: Sympathetic innervation of interscapular brown adipose tissue is not a predominant mediator of oxytocin-elicited reductions of body weight and adiposity in male diet-induced obese mice

doi: 10.3389/fendo.2024.1440070

Figure Lengend Snippet: Changes in IBAT mRNA expression following IBAT denervation.

Article Snippet: The probe for mouse Nono (Mm00834875_g1), UCP-1 (catalog no. Mm01244861_m1), UCP-2 (catalog no. Mm00627599_m1), UCP-3 (catalog no. Mm01163394_m1), β1-AR (Adrb1; catalog no. Mm00431701_s1), β2-AR (Adrb2; catalog no. Mm02524224_s1), β3-AR (Adrb3; catalog no. Mm02601819_g1), alpha-2 adrenergic receptor (Adra2a; catalog no. Mm07295458_s1),type 2 deiodinase (D2) (Dio2; catalog no. Mm00515664_m1), cytochrome c oxidase subunit 8b (Cox8b; catalog no. Mm00432648_m1), G-protein coupled receptor 120 (Gpr120; catalog no. Mm00725193_m1), bone morphogenetic protein 8b (bmp8b; catalog no. Mm00432115_g1), cell death-inducing DNA fragmentation factor alpha-like effector A (Cidea; catalog no. Mm00432554_m1), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (Ppargc1a; catalog no. Mm01208835_m1) were acquired from Thermo Fisher Scientific.

Techniques: Expressing

Journal: Frontiers in Endocrinology

Article Title: Sympathetic innervation of interscapular brown adipose tissue is not a predominant mediator of oxytocin-elicited reductions of body weight and adiposity in male diet-induced obese mice

doi: 10.3389/fendo.2024.1440070

Figure Lengend Snippet: Changes in IWAT mRNA expression following IBAT denervation.

Article Snippet: The probe for mouse Nono (Mm00834875_g1), UCP-1 (catalog no. Mm01244861_m1), UCP-2 (catalog no. Mm00627599_m1), UCP-3 (catalog no. Mm01163394_m1), β1-AR (Adrb1; catalog no. Mm00431701_s1), β2-AR (Adrb2; catalog no. Mm02524224_s1), β3-AR (Adrb3; catalog no. Mm02601819_g1), alpha-2 adrenergic receptor (Adra2a; catalog no. Mm07295458_s1),type 2 deiodinase (D2) (Dio2; catalog no. Mm00515664_m1), cytochrome c oxidase subunit 8b (Cox8b; catalog no. Mm00432648_m1), G-protein coupled receptor 120 (Gpr120; catalog no. Mm00725193_m1), bone morphogenetic protein 8b (bmp8b; catalog no. Mm00432115_g1), cell death-inducing DNA fragmentation factor alpha-like effector A (Cidea; catalog no. Mm00432554_m1), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (Ppargc1a; catalog no. Mm01208835_m1) were acquired from Thermo Fisher Scientific.

Techniques: Expressing

Journal: Frontiers in Endocrinology

Article Title: Sympathetic innervation of interscapular brown adipose tissue is not a predominant mediator of oxytocin-elicited reductions of body weight and adiposity in male diet-induced obese mice

doi: 10.3389/fendo.2024.1440070

Figure Lengend Snippet: Changes in EWAT mRNA expression following IBAT denervation.

Article Snippet: The probe for mouse Nono (Mm00834875_g1), UCP-1 (catalog no. Mm01244861_m1), UCP-2 (catalog no. Mm00627599_m1), UCP-3 (catalog no. Mm01163394_m1), β1-AR (Adrb1; catalog no. Mm00431701_s1), β2-AR (Adrb2; catalog no. Mm02524224_s1), β3-AR (Adrb3; catalog no. Mm02601819_g1), alpha-2 adrenergic receptor (Adra2a; catalog no. Mm07295458_s1),type 2 deiodinase (D2) (Dio2; catalog no. Mm00515664_m1), cytochrome c oxidase subunit 8b (Cox8b; catalog no. Mm00432648_m1), G-protein coupled receptor 120 (Gpr120; catalog no. Mm00725193_m1), bone morphogenetic protein 8b (bmp8b; catalog no. Mm00432115_g1), cell death-inducing DNA fragmentation factor alpha-like effector A (Cidea; catalog no. Mm00432554_m1), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (Ppargc1a; catalog no. Mm01208835_m1) were acquired from Thermo Fisher Scientific.

Techniques: Expressing

Journal: Molecules

Article Title: Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin

doi: 10.3390/molecules23123198

Figure Lengend Snippet: Effect of CAP-β- D gluco, Capsiate, and CAP on mRNA levels of surrogate thermogenic markers. Mean mRNA levels ± SEM of TRPV1, SiRT-1, PRDM-16, PGC-1α, BMP8b, UCP-1, and PPARα in the inguinal WAT of NCD or HFD (±CAP, Capsiate or CAP-β- d gluco)-fed wild type mice. ** represent statistical significance for p < 0.05 for n = 4 independent experiments/condition.

Article Snippet: BMP8b , (1:100) , SC-13086; Santa Cruz Biotechnology, Inc., Dallas, TX, USA.

Techniques:

Journal: Molecules

Article Title: Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin

doi: 10.3390/molecules23123198

Figure Lengend Snippet: Effect of CAP, Capsiate, and CAP-β- d gluco on thermogenic protein. A . Representative western blots showing the expression Of TRPV1, PPARα, BMP8b, UCP-1, SiRT-1, PRDM-16, and PGC-1𝛼 in the inguinal WAT of NCD or HFD (±CAP, Capsiate or CAP-β- d gluco)-fed wild type mice. B . Mean protein intensities ± SEM for n = 3 experiments/condition. ** represent statistical significance for p < 0.05.

Article Snippet: BMP8b , (1:100) , SC-13086; Santa Cruz Biotechnology, Inc., Dallas, TX, USA.

Techniques: Western Blot, Expressing

Journal: Molecules

Article Title: Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin

doi: 10.3390/molecules23123198

Figure Lengend Snippet: Dilutions of antibodies used for immunoblotting experiments.

Article Snippet: BMP8b , (1:100) , SC-13086; Santa Cruz Biotechnology, Inc., Dallas, TX, USA.

Techniques: Western Blot

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: Comparison between the upregulated genes in the bone marrow of stage III and IV gastric cancer patients.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Comparison

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: Expression levels of BMP8B mRNA in the bone marrow and peripheral blood of gastric cancer patients. (A) The expression levels of BMP8B mRNA in the bone marrow of 355 gastric cancer patients by TNM stage. The mRNA expression levels of BMP8B were normalized to those of GAPDH . The TNM stage was established based on the American Joint Committee on Cancer/International Union Against Cancer (AJCC/IUCC) staging system. Stage I, n=163; stage II, n= 4; stage III, n=77; and stage IV, n=41. P-values were estimated using the Mann-Whitney U test. (B) The correlation between BMP8B mRNA expression in 295 paired bone marrow and peripheral blood samples. A correlation coefficient (r) and the corresponding P-value for this correlation were estimated by Spearman’s correlation. BMP8B, bone morphogenic protein 8B; GAPDH , glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Expressing, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: BMP8B mRNA expression levels of bone marrow and clinicopathological factors in 355 gastric cancer patients.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: Immunohistochemical staining of BMP8B in human gastric cancer. (A) Hematoxylin-eosin (HE) staining of the gastric mucosa. (B) High BMP8B protein expression in gastric cancer cells infiltrating the gastric mucosa. (C) HE staining of the muscularis propria. (D) High BMP8B protein expression in gastric cancer cells infiltrating the muscularis propria. Scale bars, 100 μm. BMP8B, bone morphogenetic protein 8B.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: Kaplan-Meier survival analysis of cancer-specific survival in 144 gastric cancer patients comparing the high BMP8B expression group and the low BMP8B expression group using the log-rank test.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Gene expression of bone morphogenic protein 8B in the primary site, peripheral blood and bone marrow of patients with gastric cancer

doi: 10.3892/ol.2013.1392

Figure Lengend Snippet: Multivariate analysis of prognostic factors in gastric cancer following a curative resection.

Article Snippet: The BMP8B monoclonal antibody (1:100 dilution; Abnova, Tapei, Taiwan) was used as the primary antibody in the present study.

Techniques: Expressing