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Image Search Results
Journal: Nature Communications
Article Title: Transgenerational inheritance of diminished ovarian reserve triggered by prenatal propylparaben exposure in mice
doi: 10.1038/s41467-025-63440-z
Figure Lengend Snippet: a Representative ovarian images for TUNEL staining of F1–F3 offspring mice in Con and PrP groups at 12 w. b The number of TUNEL-positive cells per 1 mm 2 in ovarian sections of F1–F3 offspring mice in Con and PrP groups ( n = 5 biological replicates). Data are presented as mean ± SEM (unpaired two-tailed t -test). c The number of abnormal mitochondria in mice ovaries of F1–F3 generation ( n = 3 biological replicates, two non-overlapping microscope fields per ovary). Data are presented as mean ± SEM (unpaired two-tailed t -test). d Representative TEM images of mice ovaries in F3 generation. Yellow arrow: synapses of granulosa cells. M: mitochondria. The experiment was repeated three times. e Schematic illustration of the ovulation induction. f Representative oocyte images after ovulation induction. g The number of oocytes of F1 and F3 offspring mice in Con and PrP groups after ovulation induction (F1 n = 9; F3 Con n = 6, PrP n = 5 biological replicates). Data are presented as mean ± SEM (unpaired two-tailed t -test). h Representative IHC images showing immunoreactivity against BMP15 in F1, F2 and F3 offspring ovaries. i IHC scores based on IOD/Area of anti-BMP15 in F1, F2 and F3 offspring ovaries ( n = 5 biological replicates). Data are presented as mean ± SEM (unpaired two-tailed t -test). Source data are provided as a Source Data file. Figure 2e is created in BioRender. Wang, S. (2025) https://BioRender.com/bz8sq44 .
Article Snippet: The Antibodies were purchased from different companies: Cell Signaling Technology for ERK (Extracellular Signal-Regulated Kinase; 4695T), p-ERK (Phosphorylated ERK; 4370T), p38 (p38 Mitogen-Activated Protein Kinase; 8690T), p-p38 (Phosphorylated p38; 4511T), JNK (c-Jun N-terminal Kinase; 9252T), and p-JNK (Phosphorylated JNK; 4668T); Abclonal for β-ACTIN (AC038), active-Caspase3 (A19654), BCL-2 (A19693), BAX (A12009), SOD2 (A19576),
Techniques: TUNEL Assay, Staining, Two Tailed Test, Microscopy
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Journal: Scientific Reports
Article Title: Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes
doi: 10.1038/s41598-020-69939-3
Figure Lengend Snippet: Genes evaluated by RT-qPCR using Applied BiosystemsTaqMan Assay
Article Snippet:
Techniques: RNA Binding Assay, Histone Deacetylase Assay, Binding Assay
Journal: International Journal of Molecular Sciences
Article Title: Long-Term Changes in Ovarian Follicles of Gilts Exposed Neonatally to Methoxychlor: Effects on Oocyte-Derived Factors, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, and Cognate Receptors
doi: 10.3390/ijms23052780
Figure Lengend Snippet: List of primary antibodies used for Western blot and immunohistochemistry.
Article Snippet: Anti-BMP15 , 1:500 , 1:50 ,
Techniques: Western Blot, Immunohistochemistry