bmk 220 Search Results


erk5  (Proteintech)
93
Proteintech erk5
ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, <t>ERK5,</t> JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
Erk5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erk5/product/Proteintech
Average 93 stars, based on 1 article reviews
erk5 - by Bioz Stars, 2026-05
93/100 stars
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binder material bmkd5-1  (Dyckerhoff GmbH)
90
Dyckerhoff GmbH binder material bmkd5-1
ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, <t>ERK5,</t> JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
Binder Material Bmkd5 1, supplied by Dyckerhoff GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/binder material bmkd5-1/product/Dyckerhoff GmbH
Average 90 stars, based on 1 article reviews
binder material bmkd5-1 - by Bioz Stars, 2026-05
90/100 stars
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mouse on mouse m o m basic kit  (Vector Laboratories)
N/A
M O M Kit Mouse On Mouse Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue The inability of an anti mouse secondary antibody to
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m.o.m.(r) (mouse-on-mouse) basic immunodetection kit  (Novus Biologicals)
N/A
M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit
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Image Search Results


ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.

Journal: Redox Biology

Article Title: p47 phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis

doi: 10.1016/j.redox.2020.101569

Figure Lengend Snippet: ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Immunoblotting assay involves antibodies against p47 phox (Santa Cruz Biotechnology); NOX1 and NOX2 (Proteintech); p67 phox (Abcam); NOXO1 (ABclonal Technology); Rac (Santa Cruz Biotechnology); Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology); PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology); VASP (anti-Ser157, Affinity Biosciences; anti-Ser239 and pan-VASP, Cell Signaling Technology); ERK1/2 (anti-Thr202/Tyr204 and pan-ERK1/2, Cell Signaling Technology); p38 MAPK (anti-Thr180/Tyr182, Cell Signaling Technology); ERK5 (anti-Thr218/Tyr220, Cell Signaling Technology; pan-ERK5, Proteintech); JNK (anti-Thr183/Tyr185, Cell Signaling Technology; pan-JNK, Affinity Biosciences); AKT (anti-Thr308, Cell Signaling Technology; pan-AKT, Affinity Biosciences); c-PLA2 (anti-Ser505 and pan-c-PLA2, Affinity Biosciences); NOX1 (Novus Biologicals); NOX2 (Abcam).

Techniques: Phospho-proteomics, Western Blot, Expressing