Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: Increasing rate of daptomycin non-susceptible strains of Staphylococcus aureus in patients with atopic dermatitis

doi: 10.5114/ada.2017.72460

Figure Lengend Snippet: Susceptibility profile to Daptomycin of tested Staphylococcus aureus strains isolated from patients with atopic dermatitis (AD). Minimal inhibitory concentration was determined using the broth-microdilution method (BMD) with and without Ca 2+ (50 mg/) supplementation (148 strains). Daptomycin non-susceptible strains (MIC > 1 μg/ml) were selected for Etest confirmation (81 strains)

Article Snippet: BMD was conducted in reference to the Clinical and Laboratory Standards Institute recommendations.

Techniques: Isolation, Concentration Assay

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: Increasing rate of daptomycin non-susceptible strains of Staphylococcus aureus in patients with atopic dermatitis

doi: 10.5114/ada.2017.72460

Figure Lengend Snippet: MIC50 and MIC90 values to Daptomycin of tested Staphylococcus aureus strains isolated from patients with AD. Minimal inhibitory concentration was determined using the broth-microdilution method (BMD) with and without Ca 2+ (50 mg/l) supplementation (148 strains). Daptomycin non-susceptible strains (MIC > 1 μg/ml) were selected for Etest confirmation (81 strains)

Article Snippet: BMD was conducted in reference to the Clinical and Laboratory Standards Institute recommendations.

Techniques: Isolation, Concentration Assay

Journal: Communications Biology

Article Title: The antimicrobial potential of cannabidiol

doi: 10.1038/s42003-020-01530-y

Figure Lengend Snippet: a Structure of CBD. b MIC 90 distribution of CBD against 132 Australian S. aureus isolates. Cumulative percentage of isolates below or equal to a given MIC are indicated. Data are n = 4 biologically independent samples for MIC of each isolate. c MIC 90 distribution of CBD against 100 USA S. aureus isolates. Cumulative number of isolates below or equal to a given MIC are indicated. Data are n = 1 for MIC of each isolate. d Time-kill assay of CBD against MRSA ATCC 43300. Data are mean ± SD for n = 4 biologically independent samples. e Broth microdilution MIC distribution of CBD against 30 N. gonorrhoeae isolates. Cumulative number of isolates below or equal to a given MIC are indicated. Data are n = 1. f Agar microdilution MIC distribution of CBD against 26 N. gonorrhoeae isolates. Cumulative number of isolates below or equal to a given MIC are indicated. Data are n = 3 independent experiments.

Article Snippet: Antimicrobial activity of CBD was tested against a number of bacterial strains by broth microdilution (BMD) minimum inhibitory concentration (MIC) assays using procedures described by Clinical and Laboratory Standards Institute (CLSI) – .

Techniques: Time-Kill Assay

Journal: Microbiology Spectrum

Article Title: Clinical and microbiological analyses of colistin-resistant strains among carbapenem-resistant Enterobacter cloacae complex clinical isolates

doi: 10.1128/spectrum.01604-24

Figure Lengend Snippet: Antibiotic susceptibility test results using broth microdilution method in carbapenem-resistant Enterobacter cloacae complex isolates

Article Snippet: The MICs were determined using the BMD and agar dilution method for fosfomycin, and the susceptibility breakpoints were interpreted in accordance with the Clinical and Laboratory Standards Institute (CLSI) guideline , except for that of tigecycline.

Techniques:

Journal: Microbial Genomics

Article Title: Functional analysis of the first complete genome sequence of a multidrug resistant sequence type 2 Staphylococcus epidermidis

doi: 10.1099/mgen.0.000077

Figure Lengend Snippet: BPH0662 - a clinically significant, multidrug-resistant S. epidermidis. (a) Summary of case patient’s clinical course. (b) Magnetic resonance imaging (MRI) slices of the patient’s brain, demonstrating a large cerebral abscess (red arrow) formed around the tip of an external ventricular drain (EVD) device (yellow arrow). (c) Vancomycin population analysis profile of three S. epidermidis isolates from the case patient compared with reference strains Mu3 [heterogeneous vancomycin intermediate S. aureus (hVISA)] and Mu50 [vancomycin intermediate S. aureus (VISA)]. (d) Correlation of phenotypic susceptibility testing for case patient isolates with the BPH0662 resistome. L, left; R, right; VP, ventriculoperitoneal; CSF, cerebrospinal fluid; PEN, penicillin; FLX, flucloxacillin; COT, cotrimixazole; GEN, gentamicin; CIP, ciprofloxacin; ERY, erythromycin; CLI, clindamycin; FA, fusidic acid; RIF, rifampicin; TEC, tecoplanin; VAN, vancomycin; DAP, daptomycin; TET, tetracycline; LZD, linezolid; BMD, broth microdilution; MET, macromethod Etest; PAP/AUC, population analysis profile/ area under the curve (compared with Mu3 hVISA reference).

Article Snippet: BMD MICs for vancomycin and rifampicin were determined as recommended by the Clinical and Laboratory Standards Institute ( ).

Techniques: Magnetic Resonance Imaging