biotin label Search Results


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Novus Biologicals lightning link rapid biotin antibody labeling kit
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Role of Toll-like receptor (TLR)-2 and <t>TLR4</t> in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
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Role of Toll-like receptor (TLR)-2 and <t>TLR4</t> in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
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Elabscience Biotechnology biotin label kit
Role of Toll-like receptor (TLR)-2 and <t>TLR4</t> in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
Biotin Label Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity biotin goat anti human f ab 2 antibody coated t cells
Role of Toll-like receptor (TLR)-2 and <t>TLR4</t> in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
Biotin Goat Anti Human F Ab 2 Antibody Coated T Cells, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Eagle Biosciences biotinylated igfbp 3
Role of Toll-like receptor (TLR)-2 and <t>TLR4</t> in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
Biotinylated Igfbp 3, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Role of Toll-like receptor (TLR)-2 and TLR4 in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).

Journal: Frontiers in Immunology

Article Title: Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells

doi: 10.3389/fimmu.2018.02178

Figure Lengend Snippet: Role of Toll-like receptor (TLR)-2 and TLR4 in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).

Article Snippet: Unlabeled anti-porcine TLR2 and TLR4-rabbit IgG antibodies (Biolegend, San Diego, CA) were used to block TLRs expression in PIE cells as described previously ( ).

Techniques: Activity Assay, Incubation, Expressing, Control, Blocking Assay