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Image Search Results
Journal: Frontiers in Immunology
Article Title: Genomic Characterization of Lactobacillus delbrueckii TUA4408L and Evaluation of the Antiviral Activities of its Extracellular Polysaccharides in Porcine Intestinal Epithelial Cells
doi: 10.3389/fimmu.2018.02178
Figure Lengend Snippet: Role of Toll-like receptor (TLR)-2 and TLR4 in the immunomodulatory activity of exopolysaccharides (EPS) from Lactobacillus delbrueckii subsp. delbrueckii TUA4408L in porcine intestinal epithelial (PIE) cells. EPS was fractionated into neutral (NSP) and acidic (APS) EPS by standard chromatographic methods. PIE cells were incubated with anti-TLR2 or anti-TLR4 antibodies, treated with lactobacilli, APS or NPS for 48 h and, then stimulated with the TLR3 agonist poly(I:C). The expression of interferon β (IFN-β), interleukin 6 (IL-6), IL-8, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor (TNF-α) was evaluated 12 h after poly(I:C) challenge. The results are expressed as mean ± SD and represent data from three independent experiments ( n = 3 in each experiment). Asterisks indicate significant differences when compared to poly(I:C)-challenged control PIE cells non treated with blocking antibodies (* P < 0.05).
Article Snippet: Unlabeled anti-porcine TLR2 and
Techniques: Activity Assay, Incubation, Expressing, Control, Blocking Assay