Journal: Frontiers in Endocrinology

Article Title: Plasma-derived exosomal miRNAs as potentially novel biomarkers for type 2 diabetes mellitus with abdominal obesity

doi: 10.3389/fendo.2025.1656132

Figure Lengend Snippet: Exosome identification. The exosomes were analyzed by (A) TEM and NTA assay (B) . (C, D) The protein expression of CD9, CD81, TSG101, Calnexin, and FABP4 in exosomes was detected by Western blot assay. Cell lysate (CL) was used as a positive control for calmodulin.

Article Snippet: Western blot analysis was performed to determine the expression of CD9 (Wuhan Sanying, China, 1:2000)、CD81 (Wuhan Sanying, China, 1:1000)、Tsg101 (Wuhan Sanying, China, 1:2000)、Calnexin (Wuhan Sanying, China, 1:5000)、FABP4 (Santa Cruz, America, 1:500)、β-actin (Wuhan Sanying, China, 1:4000).

Techniques: Expressing, Western Blot, Positive Control

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p targets IL1A. A The intersection of predicted targets from TargetScan and miRDB databases. B The expression of IL1A and MEOX2 in A549, HCC827, NCI-H1299 and BEAS-2B cells. C The expression of IL1A in the serum of NSCLC patients ( n = 120) and healthy subjects ( n = 60). D The binding site of miR-323b-5p and IL1A. E Dual-luciferase reporter assay indicates that miR-323b-5p directly regulates IL1A. F Expression of IL1A in A549, HCC827, and NCI-H1299 cells after transfection with miR-323b-5p mimics. The experiment was repeated 3 times independently. ( p < 0.05*, p < 0.001***)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay, Transfection

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects A549 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). (*compared with Control, p < 0.05*. # compared with mimic NC, p < 0.05 # .)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects HCC827 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). (*compared with Control, p < 0.001***. # compared with mimic NC, p < 0.001 ### . & compared with inhibitor NC, p < 0.05 & )

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing

Journal: Discover Oncology

Article Title: The prognostic value of miR-323b-5p in non-small cell lung cancer and its mechanism of targeting IL1A in regulating the proliferation and cell cycle of non-small cell lung cancer cells

doi: 10.1007/s12672-026-04409-2

Figure Lengend Snippet: miR-323b-5p affects NCI-H1299 cell proliferation and blocks the cell cycle through IL1A. After transfection or non-transfection of A549 cells with the control mimic (mimic NC), miR-323b-5p mimic (mimic MIR), IL1A overexpression plasmid (ovIL1A), control inhibitor (inhibitor NC), miR-323b-5p inhibitor (inhibitor MIR) and IL1A knockdown plasmid (siIL1A). The transfection efficiency of each group of cells was detected by RT-qPCR ( A ), cell proliferation was detected by CCK-8 ( B ), the cell cycle status of A549 cells was detected by flow cytometry ( C ), and the expression of P21, CCND1 and CDK4 mRNA ( D ) and protein ( E ) in the cells was detected by RT-qPCR and WB respectively. The experiment was repeated 3 times independently with 3 biological replicates ( n = 3). ( # compared with mimic NC, p < 0.001 ### .)

Article Snippet: The antibodies used include: IL1A (Wuhan Sanying, 1:1000), P21 (Wuhan Sanying, 1:1500), CCND1 (Wuhan Sanying, 1:5000), cyclin dependent kinase 4 (CDK4, Wuhan Sanying, 1: 1000), β-actin (Wuhan Sanying, 1:2000).

Techniques: Transfection, Control, Over Expression, Plasmid Preparation, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing