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European Directorate for the Quality of Medicines and HealthCare
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Biostats Inc
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KU Leuven
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Image Search Results
Journal: Cell Cycle
Article Title: Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets
doi: 10.1080/15384101.2016.1146837
Figure Lengend Snippet: The combination of isoproterenol, activin A, BMP4, and bFGF induced HAP stem cells in vibrissa hair follicles to form beating cardiac-muscle tissue sheets. (A) Schematic diagram of upper parts of five hair follicles forming cardiac-muscle tissue sheets (upper panel). Bright-field image of upper part of hair follicle differentiatimg to cardiac muscle cells and immunostaining (middle panel). Red = cTnT, Blue = DAPI, Bars = 100 µm. Cardiac-muscle tissue sheet formation process (lower panel). Bar = 500 µm. (B) Immunostaining of beating cardiac-muscle tissue sheets. cTnT-positive cardiac muscle cells were extensively distributed within the sheets. Red = cTnT, Blue = DAPI. Bars = 100 µm.
Article Snippet: Beating cardiac muscle cells and
Techniques: Immunostaining
Journal: Cell Cycle
Article Title: Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets
doi: 10.1080/15384101.2016.1146837
Figure Lengend Snippet: Flow cytometry analysis of beating cardiac-muscle tissue sheets. (A) Flow cytometry analysis of harvested cardiac-muscle tissue sheets showed that the sheets contain 38.88 ± 6.64% cTnT-positive cells (left panel); 22.19 ± 6.36% CD31-positive cells (middle panel); and 29.58 ± 6.11% SMA-positive cells (right panel). (B) Intercellular Ca2+ imaging of cardiac-muscle tissue sheets. Phase contrast image of cardiac muscle cells loaded with Flou-3 (white dashed area). Images were obtained every 150 msec (left panel). Bar =100 µm. Fluo-3 image at 4 points (I, II, III, IV) showing time course of Fluo-3 intensity change (middle and left panel). (C) High rate of formation of the beating cardiac-muscle tissue sheets from HAP stem cells. Hair follicles were cultured in medium supplemented with isoproterenol, activin A, BMP4 and bFGF, where cardiac-muscle tissue sheets formed at 65.0 ± 5.77%. No cardiac tissue sheets formed in non-supplemented medium or medium supplemented with isoproterenol alone.
Article Snippet: Beating cardiac muscle cells and
Techniques: Flow Cytometry, Imaging, Cell Culture