Journal: bioRxiv

Article Title: Tyrosine phosphorylation coupling of one carbon metabolism and virulence in an endogenous pathogen

doi: 10.1101/2025.03.11.642667

Figure Lengend Snippet: a, b) Coomassie blue stain of representative SDS-PAGE of P. gingivalis cell fractions analyzed in . c) Silver stain of representative SDS-PAGE of P. gingivalis culture supernatants. The intense band is the catalytic domain from the gingipains.

Article Snippet: Proteins were visualized with Coomassie Brilliant Blue (BioRad) or silver stain (ThermoFisher).

Techniques: Staining, SDS Page, Silver Staining

Journal: European journal of biochemistry

Article Title: The relevance of N-linked glycosylation to the binding of a ligand to guanylate cyclase C.

doi: 10.1046/j.1432-1327.1999.00488.x

Figure Lengend Snippet: Fig. 2. SDS/PAGE and Western blotting of ECD6H expressed from Sf21 cells. Purified by concanavalin A and Ni-chelating affinity chromatography, and visualized by (A) silver stain, (B) a His6-tagged protein detection kit and (C) concanavalin A-conjugated HRP. PGNase F (+) and (±) indicate treatment without or with PNGase F. (D) ECD6H was treated without (lanes 1, 2) or with (lanes 3, 4) PNGase F and subsequently photoaffinity-labeled with BB-[125I-Tyr4]STp(4±17) in the absence (lanes 1, 3) and presence (lanes 2, 4) of 1026 m STp(4±17).

Article Snippet: The deglycosylation of ECD6H was confirmed using succinylatedconcanavalin A conjugated with horseradish peroxidase (HRP; Vector Lab. Inc.) and an HRP Conjugate Substrate Kit (BioRad).

Techniques: SDS Page, Western Blot, Purification, Affinity Chromatography, Silver Staining, Labeling