Journal: Nature Communications

Article Title: Plasma cell survival in the absence of B cell memory

doi: 10.1038/s41467-017-01901-w

Figure Lengend Snippet: Longitudinal analysis of antibody responses to multiple antigens after vaccination or infection. Serum antibody titers were measured at the indicated time points for a paramyxovirus that is antigenically related to measles virus (Measles), rhesus cytomegalovirus (RhCMV), adenovirus, pertussis toxin, filamentous hemagglutinin (FHA), and pertactin. Arrows indicate the dates when anti-CD20 administration was performed or when splenectomy and draining lymph nodes (LN) were surgically removed. Control animals, Rh#20923 and Rh#21169, did not have anti-CD20 treatment or surgeries performed and are represented by dashed lines. The gray shaded region below the dotted line (<200 ELISA units) represents the points in which ELISA titers become equivocal or are below the limit of detection. Control animal Rh#21169 was seronegative for measles virus antigen

Article Snippet: Antigens included tetanus toxoid (0.125 μg/ml, Cat#582231, Calbiochem), diphtheria toxin (1 μg/ml, Cat#150, List Biological Laboratories), pertussis toxin (1 μg/ml, Cat#180 List Biological laboratories) filamentous hemagglutinin (0.5 μg/ml Cat #170, List Biological Laboratories), Pertactin (0.5 μg/ml BEI Resources, NR-34571), inactivated measles-Edmonston strain (1:250 dilution, AbD Serotec, PIP013), RhCMV strain 68-1 (1:1600 dilution, an in-house, detergent extract of RhCMV 68-1-infected rhesus fibroblasts treated with 2-mercaptoethanol to reduce non-specific binding to IgG) and H 2 O 2 -inactivated adenovirus (1:500 dilution, an in-house reagent consisting of Adenovirus serotype 5 with E1/E3 deleted).

Techniques: Infection, Virus, Control, Enzyme-linked Immunosorbent Assay