Journal: Molecules

Article Title: “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

doi: 10.3390/molecules24224146

Figure Lengend Snippet: Surface modification of magnetic nanoparticles and immobilization of phenylalanine ammonia-lyase from Petroselinum crispum ( Pc PAL) onto mixed epoxy-chelate magnetic nanoparticles from cell lysate.

Article Snippet: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts.

Techniques: Modification

Journal: Molecules

Article Title: “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

doi: 10.3390/molecules24224146

Figure Lengend Snippet: Effect of the bisepoxide to ethylenediaminetetraacetic dianhydride ratio in surface functionalization on the activity of the immobilized Pc PAL biocatalyst. Lysate: crude protein mixture without immobilization; EDa: only EDa modified support; NPDGE: only NPDGE modified support; numbers: quantity of NPDGE to one unit of EDa during the surface modification. Colored bars: specific biocatalytic activity ( U B ), colored dots: activity yield ( Y A ); for the non-immobilized cell-lysate U B = U E . The measurements were performed in three replicates; the standard deviation of activity yield values was always below 5%. Experiments were performed as described in .

Article Snippet: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts.

Techniques: Activity Assay, Modification, Standard Deviation

Journal: Molecules

Article Title: “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

doi: 10.3390/molecules24224146

Figure Lengend Snippet: Comparison of Pc PAL immobilization from crude lysate or from pure enzyme solution using epoxy and optimized mixed epoxy-chelate MNPs as support [ U B (μmol × min −1 × g −1 )].

Article Snippet: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts.

Techniques: Comparison, Purification

Journal: Molecules

Article Title: “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

doi: 10.3390/molecules24224146

Figure Lengend Snippet: Comparison of the efficiency of Pc PAL immobilization [ U B (μmol × min −1 × g −1 )] with epoxy and optimized epoxy-chelate supports from protein mixtures of different target enzyme content.

Article Snippet: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts.

Techniques: Comparison, Protein Concentration

Journal: Molecules

Article Title: “Fishing and Hunting”—Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media

doi: 10.3390/molecules24224146

Figure Lengend Snippet: Effect of the nature of multiepoxide linkers on the activity and immobilization efficiency of Pc PAL biocatalysts. Bars: specific biocatalytic activity ( U B ), dots: activity yield ( Y A ); for the non-immobilized cell-lysate U B = U E . The measurements were performed in three replicates; the standard deviation of activity yield values was always below 5%. Experiments were performed as described in .

Article Snippet: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts.

Techniques: Activity Assay, Standard Deviation